Experiment: 24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 30% DO with 20% v/v dodecane overlay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0510 and PP_0511 are separated by 31 nucleotides PP_0511 and PP_0512 are separated by 0 nucleotides PP_0512 and PP_0513 are separated by 130 nucleotides
PP_0510: PP_0510 - Thioredoxin, at 598,072 to 598,944
_0510
PP_0511: PP_0511 - putative methyltransferase, at 598,976 to 599,635
_0511
PP_0512: PP_0512 - hypothetical protein, at 599,636 to 600,043
_0512
PP_0513: PP_0513 - DNA-binding transcriptional repressor NrdR-Zn2+-ATP/dATP, at 600,174 to 600,653
_0513
Position (kb) 598
599
600 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 598.192 kb on - strand, within PP_0510 at 598.232 kb on + strand, within PP_0510 at 598.233 kb on - strand, within PP_0510 at 598.311 kb on + strand, within PP_0510 at 598.311 kb on + strand, within PP_0510 at 598.466 kb on + strand, within PP_0510 at 598.466 kb on + strand, within PP_0510 at 598.516 kb on + strand, within PP_0510 at 598.517 kb on - strand, within PP_0510 at 598.606 kb on + strand, within PP_0510 at 598.606 kb on + strand, within PP_0510 at 598.606 kb on + strand, within PP_0510 at 598.607 kb on - strand, within PP_0510 at 598.607 kb on - strand, within PP_0510 at 598.607 kb on - strand, within PP_0510 at 598.607 kb on - strand, within PP_0510 at 598.607 kb on - strand, within PP_0510 at 598.926 kb on - strand at 598.926 kb on - strand at 598.966 kb on + strand at 598.966 kb on + strand at 598.967 kb on - strand at 598.977 kb on + strand at 598.978 kb on - strand at 598.978 kb on - strand at 598.979 kb on + strand at 598.979 kb on + strand at 598.979 kb on + strand at 598.980 kb on - strand at 598.980 kb on - strand at 598.980 kb on - strand at 598.980 kb on - strand at 599.001 kb on + strand at 599.079 kb on + strand, within PP_0511 at 599.080 kb on - strand, within PP_0511 at 599.080 kb on - strand, within PP_0511 at 599.092 kb on + strand, within PP_0511 at 599.179 kb on - strand, within PP_0511 at 599.179 kb on - strand, within PP_0511 at 599.241 kb on + strand, within PP_0511 at 599.242 kb on - strand, within PP_0511 at 599.242 kb on - strand, within PP_0511 at 599.242 kb on - strand, within PP_0511 at 599.246 kb on + strand, within PP_0511 at 599.246 kb on + strand, within PP_0511 at 599.246 kb on + strand, within PP_0511 at 599.247 kb on - strand, within PP_0511 at 599.425 kb on - strand, within PP_0511 at 599.466 kb on + strand, within PP_0511 at 599.543 kb on + strand, within PP_0511 at 599.544 kb on - strand, within PP_0511 at 599.637 kb on + strand at 599.637 kb on + strand at 599.637 kb on + strand at 599.638 kb on - strand at 599.638 kb on - strand at 599.638 kb on - strand at 599.800 kb on - strand, within PP_0512 at 599.800 kb on - strand, within PP_0512 at 599.943 kb on + strand, within PP_0512 at 600.075 kb on + strand at 600.076 kb on - strand at 600.103 kb on + strand at 600.103 kb on + strand at 600.104 kb on - strand at 600.104 kb on - strand at 600.558 kb on + strand at 600.558 kb on + strand, within PP_0513 at 600.558 kb on + strand, within PP_0513 at 600.558 kb on + strand, within PP_0513 at 600.579 kb on + strand, within PP_0513
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 30% DO with 20% v/v dodecane overlay remove 598,192 - PP_0510 0.14 -0.3 598,232 + PP_0510 0.18 +0.9 598,233 - PP_0510 0.18 -0.4 598,311 + PP_0510 0.27 -0.3 598,311 + PP_0510 0.27 -0.4 598,466 + PP_0510 0.45 +1.3 598,466 + PP_0510 0.45 +0.7 598,516 + PP_0510 0.51 +0.6 598,517 - PP_0510 0.51 -1.8 598,606 + PP_0510 0.61 +1.0 598,606 + PP_0510 0.61 -1.2 598,606 + PP_0510 0.61 -0.0 598,607 - PP_0510 0.61 -0.4 598,607 - PP_0510 0.61 +0.6 598,607 - PP_0510 0.61 +0.1 598,607 - PP_0510 0.61 +1.2 598,607 - PP_0510 0.61 -0.2 598,926 - -0.7 598,926 - -0.8 598,966 + -1.4 598,966 + +1.1 598,967 - -0.4 598,977 + +1.6 598,978 - -2.2 598,978 - +0.6 598,979 + -0.0 598,979 + -0.0 598,979 + +0.6 598,980 - +2.4 598,980 - +0.6 598,980 - +0.9 598,980 - +0.6 599,001 + +0.6 599,079 + PP_0511 0.16 -0.9 599,080 - PP_0511 0.16 +0.3 599,080 - PP_0511 0.16 -1.0 599,092 + PP_0511 0.18 +0.1 599,179 - PP_0511 0.31 -0.2 599,179 - PP_0511 0.31 -0.6 599,241 + PP_0511 0.40 -0.4 599,242 - PP_0511 0.40 -1.8 599,242 - PP_0511 0.40 -1.3 599,242 - PP_0511 0.40 -1.8 599,246 + PP_0511 0.41 -0.4 599,246 + PP_0511 0.41 +0.8 599,246 + PP_0511 0.41 -1.1 599,247 - PP_0511 0.41 -0.8 599,425 - PP_0511 0.68 -1.2 599,466 + PP_0511 0.74 -0.4 599,543 + PP_0511 0.86 -0.8 599,544 - PP_0511 0.86 -2.9 599,637 + -0.4 599,637 + -1.0 599,637 + -1.0 599,638 - -0.9 599,638 - -0.2 599,638 - +0.9 599,800 - PP_0512 0.40 -0.5 599,800 - PP_0512 0.40 -0.4 599,943 + PP_0512 0.75 -1.6 600,075 + +1.4 600,076 - -1.2 600,103 + -0.4 600,103 + +1.0 600,104 - -0.2 600,104 - -0.4 600,558 + -0.4 600,558 + PP_0513 0.80 +2.1 600,558 + PP_0513 0.80 -0.4 600,558 + PP_0513 0.80 +1.6 600,579 + PP_0513 0.84 -0.8
Or see this region's nucleotide sequence