Strain Fitness in Escherichia coli BW25113 around b3856

Experiment: LB Anaerobic with Chloramphenicol 0.00000390625 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntrrlA and rrfA are separated by 93 nucleotidesrrfA and mobB are separated by 269 nucleotidesmobB and mobA overlap by 19 nucleotidesmobA and yihD are separated by 69 nucleotidesyihD and yihE are separated by 76 nucleotides b3854: rrlA - 23S ribosomal RNA (NCBI), at 4,035,542 to 4,038,446 rrlA b3855: rrfA - 5S ribosomal RNA (NCBI), at 4,038,540 to 4,038,659 rrfA b3856: mobB - molybdopterin-guanine dinucleotide biosynthesis protein B (RefSeq), at 4,038,929 to 4,039,456 mobB b3857: mobA - molybdopterin-guanine dinucleotide biosynthesis protein A (NCBI), at 4,039,438 to 4,040,022 mobA b3858: yihD - hypothetical protein (NCBI), at 4,040,092 to 4,040,361 yihD b3859: yihE - predicted kinase (NCBI), at 4,040,438 to 4,041,424 yihE Position (kb) 4038 4039 4040Strain fitness (log2 ratio) -3 -2 -1 0 1at 4038.917 kb on + strandat 4038.982 kb on - strand, within mobBat 4039.000 kb on - strand, within mobBat 4039.014 kb on - strand, within mobBat 4039.036 kb on + strand, within mobBat 4039.104 kb on + strand, within mobBat 4039.104 kb on + strand, within mobBat 4039.150 kb on - strand, within mobBat 4039.192 kb on - strand, within mobBat 4039.207 kb on - strand, within mobBat 4039.226 kb on + strand, within mobBat 4039.271 kb on - strand, within mobBat 4039.272 kb on + strand, within mobBat 4039.279 kb on + strand, within mobBat 4039.282 kb on - strand, within mobBat 4039.318 kb on - strand, within mobBat 4039.318 kb on - strand, within mobBat 4039.347 kb on - strand, within mobBat 4039.414 kb on + strandat 4039.540 kb on + strand, within mobAat 4039.571 kb on - strand, within mobAat 4039.642 kb on + strand, within mobAat 4039.717 kb on - strand, within mobAat 4039.774 kb on - strand, within mobAat 4039.774 kb on - strand, within mobAat 4039.801 kb on - strand, within mobAat 4039.821 kb on + strand, within mobAat 4039.821 kb on + strand, within mobAat 4039.821 kb on + strand, within mobAat 4039.930 kb on + strand, within mobAat 4039.962 kb on + strand, within mobAat 4039.968 kb on - strandat 4039.968 kb on - strandat 4040.054 kb on + strandat 4040.080 kb on + strandat 4040.082 kb on - strandat 4040.127 kb on + strand, within yihDat 4040.236 kb on + strand, within yihDat 4040.236 kb on + strand, within yihDat 4040.249 kb on + strand, within yihDat 4040.339 kb on + strandat 4040.405 kb on - strandat 4040.436 kb on + strandat 4040.436 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Anaerobic with Chloramphenicol 0.00000390625 mM
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4,038,917 + +1.1
4,038,982 - mobB b3856 0.10 +0.4
4,039,000 - mobB b3856 0.13 -0.3
4,039,014 - mobB b3856 0.16 +0.3
4,039,036 + mobB b3856 0.20 +0.1
4,039,104 + mobB b3856 0.33 +0.2
4,039,104 + mobB b3856 0.33 -0.1
4,039,150 - mobB b3856 0.42 +0.7
4,039,192 - mobB b3856 0.50 -0.4
4,039,207 - mobB b3856 0.53 -0.1
4,039,226 + mobB b3856 0.56 +0.2
4,039,271 - mobB b3856 0.65 -0.2
4,039,272 + mobB b3856 0.65 +0.5
4,039,279 + mobB b3856 0.66 -0.5
4,039,282 - mobB b3856 0.67 -0.1
4,039,318 - mobB b3856 0.74 -0.9
4,039,318 - mobB b3856 0.74 +0.5
4,039,347 - mobB b3856 0.79 -0.2
4,039,414 + +0.5
4,039,540 + mobA b3857 0.17 -1.3
4,039,571 - mobA b3857 0.23 -1.7
4,039,642 + mobA b3857 0.35 -1.7
4,039,717 - mobA b3857 0.48 -1.4
4,039,774 - mobA b3857 0.57 -0.1
4,039,774 - mobA b3857 0.57 -1.5
4,039,801 - mobA b3857 0.62 -1.9
4,039,821 + mobA b3857 0.65 -1.5
4,039,821 + mobA b3857 0.65 +0.2
4,039,821 + mobA b3857 0.65 -3.5
4,039,930 + mobA b3857 0.84 -1.4
4,039,962 + mobA b3857 0.90 -0.8
4,039,968 - -0.1
4,039,968 - -2.8
4,040,054 + -0.4
4,040,080 + -0.1
4,040,082 - -1.4
4,040,127 + yihD b3858 0.13 -1.4
4,040,236 + yihD b3858 0.53 +0.2
4,040,236 + yihD b3858 0.53 +0.2
4,040,249 + yihD b3858 0.58 -0.8
4,040,339 + -0.3
4,040,405 - -0.4
4,040,436 + +0.3
4,040,436 + -0.2

Or see this region's nucleotide sequence