Experiment: LB Anaerobic with Benzethonium chloride 0.00003125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ptsA and frwC are separated by 308 nucleotides frwC and frwB are separated by 14 nucleotides frwB and pflD are separated by 50 nucleotides
b3947: ptsA - fused predicted PTS enzymes: Hpr component/enzyme I component/enzyme IIA component (NCBI), at 4,137,743 to 4,140,244
ptsA
b3949: frwC - predicted enzyme IIC component of PTS (NCBI), at 4,140,553 to 4,141,632
frwC
b3950: frwB - predicted enzyme IIB component of PTS (NCBI), at 4,141,647 to 4,141,967
frwB
b3951: pflD - predicted formate acetyltransferase 2 (pyruvate formate lyase II) (NCBI), at 4,142,018 to 4,144,315
pflD
Position (kb)
4140
4141
4142 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 4139.709 kb on - strand, within ptsA at 4139.770 kb on - strand, within ptsA at 4139.782 kb on - strand, within ptsA at 4139.782 kb on - strand, within ptsA at 4139.787 kb on - strand, within ptsA at 4139.878 kb on + strand, within ptsA at 4139.888 kb on - strand, within ptsA at 4139.891 kb on + strand, within ptsA at 4140.001 kb on + strand at 4140.001 kb on + strand at 4140.009 kb on + strand at 4140.096 kb on - strand at 4140.130 kb on + strand at 4140.133 kb on - strand at 4140.133 kb on - strand at 4140.196 kb on + strand at 4140.196 kb on + strand at 4140.204 kb on - strand at 4140.206 kb on + strand at 4140.206 kb on + strand at 4140.285 kb on - strand at 4140.295 kb on + strand at 4140.539 kb on + strand at 4140.667 kb on + strand, within frwC at 4140.667 kb on + strand, within frwC at 4140.685 kb on + strand, within frwC at 4140.685 kb on + strand, within frwC at 4140.685 kb on + strand, within frwC at 4140.693 kb on - strand, within frwC at 4140.756 kb on + strand, within frwC at 4140.805 kb on + strand, within frwC at 4140.808 kb on - strand, within frwC at 4140.827 kb on + strand, within frwC at 4140.827 kb on + strand, within frwC at 4140.830 kb on + strand, within frwC at 4140.895 kb on + strand, within frwC at 4140.895 kb on + strand, within frwC at 4140.951 kb on + strand, within frwC at 4141.002 kb on - strand, within frwC at 4141.010 kb on + strand, within frwC at 4141.010 kb on + strand, within frwC at 4141.051 kb on - strand, within frwC at 4141.066 kb on + strand, within frwC at 4141.069 kb on + strand, within frwC at 4141.069 kb on + strand, within frwC at 4141.069 kb on + strand, within frwC at 4141.092 kb on - strand, within frwC at 4141.105 kb on + strand, within frwC at 4141.130 kb on - strand, within frwC at 4141.133 kb on + strand, within frwC at 4141.154 kb on + strand, within frwC at 4141.167 kb on + strand, within frwC at 4141.191 kb on - strand, within frwC at 4141.194 kb on - strand, within frwC at 4141.194 kb on - strand, within frwC at 4141.218 kb on + strand, within frwC at 4141.218 kb on + strand, within frwC at 4141.255 kb on + strand, within frwC at 4141.255 kb on + strand, within frwC at 4141.311 kb on + strand, within frwC at 4141.311 kb on + strand, within frwC at 4141.320 kb on - strand, within frwC at 4141.320 kb on - strand, within frwC at 4141.320 kb on - strand, within frwC at 4141.441 kb on - strand, within frwC at 4141.448 kb on - strand, within frwC at 4141.448 kb on - strand, within frwC at 4141.449 kb on + strand, within frwC at 4141.450 kb on + strand, within frwC at 4141.494 kb on + strand, within frwC at 4141.549 kb on - strand at 4141.549 kb on - strand at 4141.676 kb on + strand at 4141.692 kb on - strand, within frwB at 4141.692 kb on - strand, within frwB at 4141.816 kb on + strand, within frwB at 4141.832 kb on + strand, within frwB at 4141.832 kb on + strand, within frwB at 4141.969 kb on + strand at 4142.009 kb on + strand at 4142.028 kb on + strand at 4142.092 kb on + strand at 4142.204 kb on - strand at 4142.223 kb on + strand at 4142.225 kb on + strand at 4142.282 kb on + strand, within pflD at 4142.282 kb on + strand, within pflD at 4142.357 kb on - strand, within pflD at 4142.357 kb on - strand, within pflD at 4142.370 kb on - strand, within pflD at 4142.370 kb on - strand, within pflD at 4142.410 kb on + strand, within pflD at 4142.461 kb on + strand, within pflD at 4142.461 kb on + strand, within pflD at 4142.476 kb on - strand, within pflD at 4142.476 kb on - strand, within pflD at 4142.497 kb on - strand, within pflD at 4142.497 kb on - strand, within pflD at 4142.499 kb on + strand, within pflD at 4142.528 kb on - strand, within pflD at 4142.602 kb on + strand, within pflD
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Benzethonium chloride 0.00003125 mM remove 4,139,709 - ptsA b3947 0.79 -0.9 4,139,770 - ptsA b3947 0.81 +1.0 4,139,782 - ptsA b3947 0.81 -2.5 4,139,782 - ptsA b3947 0.81 -0.2 4,139,787 - ptsA b3947 0.82 +2.0 4,139,878 + ptsA b3947 0.85 -1.2 4,139,888 - ptsA b3947 0.86 +2.0 4,139,891 + ptsA b3947 0.86 +1.7 4,140,001 + +1.1 4,140,001 + +2.3 4,140,009 + +1.8 4,140,096 - -0.0 4,140,130 + +0.4 4,140,133 - -0.1 4,140,133 - -1.7 4,140,196 + +1.3 4,140,196 + +0.3 4,140,204 - +0.1 4,140,206 + +0.9 4,140,206 + +1.9 4,140,285 - -0.3 4,140,295 + -0.7 4,140,539 + +2.3 4,140,667 + frwC b3949 0.11 -1.4 4,140,667 + frwC b3949 0.11 +0.3 4,140,685 + frwC b3949 0.12 -0.3 4,140,685 + frwC b3949 0.12 +1.0 4,140,685 + frwC b3949 0.12 -2.3 4,140,693 - frwC b3949 0.13 +1.5 4,140,756 + frwC b3949 0.19 +1.0 4,140,805 + frwC b3949 0.23 +0.3 4,140,808 - frwC b3949 0.24 +1.3 4,140,827 + frwC b3949 0.25 +0.8 4,140,827 + frwC b3949 0.25 +2.1 4,140,830 + frwC b3949 0.26 +0.2 4,140,895 + frwC b3949 0.32 -1.9 4,140,895 + frwC b3949 0.32 +2.1 4,140,951 + frwC b3949 0.37 -0.7 4,141,002 - frwC b3949 0.42 -0.1 4,141,010 + frwC b3949 0.42 -0.4 4,141,010 + frwC b3949 0.42 -2.9 4,141,051 - frwC b3949 0.46 +0.3 4,141,066 + frwC b3949 0.47 -2.3 4,141,069 + frwC b3949 0.48 -0.0 4,141,069 + frwC b3949 0.48 -1.9 4,141,069 + frwC b3949 0.48 +0.3 4,141,092 - frwC b3949 0.50 +0.3 4,141,105 + frwC b3949 0.51 +1.9 4,141,130 - frwC b3949 0.53 -0.1 4,141,133 + frwC b3949 0.54 +3.0 4,141,154 + frwC b3949 0.56 +0.1 4,141,167 + frwC b3949 0.57 +0.4 4,141,191 - frwC b3949 0.59 -3.0 4,141,194 - frwC b3949 0.59 +1.3 4,141,194 - frwC b3949 0.59 +1.7 4,141,218 + frwC b3949 0.62 +1.0 4,141,218 + frwC b3949 0.62 -2.5 4,141,255 + frwC b3949 0.65 -2.3 4,141,255 + frwC b3949 0.65 +0.3 4,141,311 + frwC b3949 0.70 -2.8 4,141,311 + frwC b3949 0.70 +2.1 4,141,320 - frwC b3949 0.71 -1.9 4,141,320 - frwC b3949 0.71 -1.5 4,141,320 - frwC b3949 0.71 +0.9 4,141,441 - frwC b3949 0.82 +2.6 4,141,448 - frwC b3949 0.83 +2.9 4,141,448 - frwC b3949 0.83 +1.0 4,141,449 + frwC b3949 0.83 -1.6 4,141,450 + frwC b3949 0.83 -0.6 4,141,494 + frwC b3949 0.87 -0.1 4,141,549 - +2.1 4,141,549 - +0.9 4,141,676 + +0.8 4,141,692 - frwB b3950 0.14 +1.0 4,141,692 - frwB b3950 0.14 -1.4 4,141,816 + frwB b3950 0.53 +0.6 4,141,832 + frwB b3950 0.58 +1.0 4,141,832 + frwB b3950 0.58 -1.5 4,141,969 + +1.3 4,142,009 + +0.9 4,142,028 + +1.4 4,142,092 + -0.4 4,142,204 - +0.3 4,142,223 + +1.4 4,142,225 + -2.7 4,142,282 + pflD b3951 0.11 -2.6 4,142,282 + pflD b3951 0.11 +2.1 4,142,357 - pflD b3951 0.15 +1.0 4,142,357 - pflD b3951 0.15 +0.7 4,142,370 - pflD b3951 0.15 +1.5 4,142,370 - pflD b3951 0.15 -2.0 4,142,410 + pflD b3951 0.17 -0.5 4,142,461 + pflD b3951 0.19 +0.1 4,142,461 + pflD b3951 0.19 +1.5 4,142,476 - pflD b3951 0.20 +1.7 4,142,476 - pflD b3951 0.20 -1.0 4,142,497 - pflD b3951 0.21 +0.1 4,142,497 - pflD b3951 0.21 -2.4 4,142,499 + pflD b3951 0.21 -0.5 4,142,528 - pflD b3951 0.22 -0.2 4,142,602 + pflD b3951 0.25 +1.1
Or see this region's nucleotide sequence