Strain Fitness in Escherichia coli BW25113 around b3946

Experiment: LB Anaerobic with Benzethonium chloride 0.00003125 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntgldA and fsaB are separated by 10 nucleotidesfsaB and ptsA are separated by 11 nucleotides b3945: gldA - glycerol dehydrogenase, (NAD) (VIMSS), at 4,135,955 to 4,137,058 gldA b3946: fsaB - fructose-6-phosphate aldolase 2 (NCBI), at 4,137,069 to 4,137,731 fsaB b3947: ptsA - fused predicted PTS enzymes: Hpr component/enzyme I component/enzyme IIA component (NCBI), at 4,137,743 to 4,140,244 ptsA Position (kb) 4137 4138Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3at 4136.159 kb on + strand, within gldAat 4136.201 kb on + strand, within gldAat 4136.229 kb on - strand, within gldAat 4136.229 kb on - strand, within gldAat 4136.248 kb on + strand, within gldAat 4136.248 kb on + strand, within gldAat 4136.260 kb on - strand, within gldAat 4136.260 kb on - strand, within gldAat 4136.277 kb on - strand, within gldAat 4136.353 kb on + strand, within gldAat 4136.361 kb on - strand, within gldAat 4136.390 kb on - strand, within gldAat 4136.390 kb on - strand, within gldAat 4136.435 kb on + strand, within gldAat 4136.444 kb on - strand, within gldAat 4136.454 kb on - strand, within gldAat 4136.474 kb on - strand, within gldAat 4136.474 kb on - strand, within gldAat 4136.474 kb on - strand, within gldAat 4136.474 kb on - strand, within gldAat 4136.508 kb on + strand, within gldAat 4136.557 kb on - strand, within gldAat 4136.562 kb on + strand, within gldAat 4136.638 kb on + strand, within gldAat 4136.667 kb on - strand, within gldAat 4136.670 kb on + strand, within gldAat 4136.672 kb on - strand, within gldAat 4136.690 kb on - strand, within gldAat 4136.725 kb on + strand, within gldAat 4136.733 kb on - strand, within gldAat 4136.733 kb on - strand, within gldAat 4136.733 kb on - strand, within gldAat 4136.738 kb on - strand, within gldAat 4136.738 kb on - strand, within gldAat 4136.784 kb on + strand, within gldAat 4136.784 kb on + strand, within gldAat 4136.812 kb on - strand, within gldAat 4136.812 kb on - strand, within gldAat 4136.916 kb on + strandat 4136.970 kb on + strandat 4136.970 kb on + strandat 4137.030 kb on - strandat 4137.061 kb on - strandat 4137.061 kb on - strandat 4137.105 kb on - strandat 4137.105 kb on - strandat 4137.105 kb on - strandat 4137.196 kb on + strand, within fsaBat 4137.214 kb on + strand, within fsaBat 4137.215 kb on - strand, within fsaBat 4137.234 kb on - strand, within fsaBat 4137.288 kb on + strand, within fsaBat 4137.288 kb on + strand, within fsaBat 4137.288 kb on + strand, within fsaBat 4137.291 kb on - strand, within fsaBat 4137.296 kb on - strand, within fsaBat 4137.296 kb on - strand, within fsaBat 4137.344 kb on - strand, within fsaBat 4137.389 kb on - strand, within fsaBat 4137.389 kb on - strand, within fsaBat 4137.393 kb on + strand, within fsaBat 4137.396 kb on + strand, within fsaBat 4137.401 kb on - strand, within fsaBat 4137.401 kb on - strand, within fsaBat 4137.404 kb on - strand, within fsaBat 4137.404 kb on - strand, within fsaBat 4137.404 kb on - strand, within fsaBat 4137.404 kb on - strand, within fsaBat 4137.468 kb on - strand, within fsaBat 4137.507 kb on + strand, within fsaBat 4137.525 kb on - strand, within fsaBat 4137.525 kb on - strand, within fsaBat 4137.539 kb on - strand, within fsaBat 4137.539 kb on - strand, within fsaBat 4137.552 kb on + strand, within fsaBat 4137.610 kb on + strand, within fsaBat 4137.647 kb on + strand, within fsaBat 4137.703 kb on + strandat 4137.703 kb on - strandat 4137.705 kb on - strandat 4137.866 kb on - strandat 4137.866 kb on - strandat 4137.900 kb on - strandat 4137.900 kb on - strandat 4137.900 kb on - strandat 4138.103 kb on - strand, within ptsAat 4138.103 kb on - strand, within ptsAat 4138.112 kb on - strand, within ptsAat 4138.124 kb on - strand, within ptsAat 4138.205 kb on - strand, within ptsAat 4138.205 kb on - strand, within ptsAat 4138.208 kb on - strand, within ptsAat 4138.259 kb on + strand, within ptsAat 4138.351 kb on + strand, within ptsAat 4138.396 kb on + strand, within ptsAat 4138.398 kb on + strand, within ptsAat 4138.398 kb on + strand, within ptsAat 4138.444 kb on - strand, within ptsAat 4138.444 kb on - strand, within ptsAat 4138.446 kb on + strand, within ptsAat 4138.459 kb on + strand, within ptsAat 4138.467 kb on - strand, within ptsAat 4138.662 kb on - strand, within ptsAat 4138.705 kb on + strand, within ptsAat 4138.705 kb on + strand, within ptsA

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Anaerobic with Benzethonium chloride 0.00003125 mM
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4,136,159 + gldA b3945 0.18 +0.2
4,136,201 + gldA b3945 0.22 +0.6
4,136,229 - gldA b3945 0.25 +1.4
4,136,229 - gldA b3945 0.25 -2.8
4,136,248 + gldA b3945 0.27 +0.1
4,136,248 + gldA b3945 0.27 -2.3
4,136,260 - gldA b3945 0.28 -1.0
4,136,260 - gldA b3945 0.28 +1.2
4,136,277 - gldA b3945 0.29 +0.2
4,136,353 + gldA b3945 0.36 +0.5
4,136,361 - gldA b3945 0.37 -1.1
4,136,390 - gldA b3945 0.39 -1.9
4,136,390 - gldA b3945 0.39 +2.3
4,136,435 + gldA b3945 0.43 +1.0
4,136,444 - gldA b3945 0.44 +2.7
4,136,454 - gldA b3945 0.45 -0.8
4,136,474 - gldA b3945 0.47 -0.4
4,136,474 - gldA b3945 0.47 +2.6
4,136,474 - gldA b3945 0.47 -1.1
4,136,474 - gldA b3945 0.47 +1.5
4,136,508 + gldA b3945 0.50 -1.9
4,136,557 - gldA b3945 0.55 +0.2
4,136,562 + gldA b3945 0.55 +0.2
4,136,638 + gldA b3945 0.62 -2.4
4,136,667 - gldA b3945 0.64 -0.1
4,136,670 + gldA b3945 0.65 -1.4
4,136,672 - gldA b3945 0.65 +0.0
4,136,690 - gldA b3945 0.67 -0.3
4,136,725 + gldA b3945 0.70 +0.7
4,136,733 - gldA b3945 0.70 -2.1
4,136,733 - gldA b3945 0.70 +1.9
4,136,733 - gldA b3945 0.70 +0.1
4,136,738 - gldA b3945 0.71 -2.8
4,136,738 - gldA b3945 0.71 +1.5
4,136,784 + gldA b3945 0.75 -1.3
4,136,784 + gldA b3945 0.75 +1.2
4,136,812 - gldA b3945 0.78 +0.3
4,136,812 - gldA b3945 0.78 +3.4
4,136,916 + +1.9
4,136,970 + -2.8
4,136,970 + -0.9
4,137,030 - +1.3
4,137,061 - -4.3
4,137,061 - -2.3
4,137,105 - -0.8
4,137,105 - +2.1
4,137,105 - -0.4
4,137,196 + fsaB b3946 0.19 +0.3
4,137,214 + fsaB b3946 0.22 -3.4
4,137,215 - fsaB b3946 0.22 -0.3
4,137,234 - fsaB b3946 0.25 -3.3
4,137,288 + fsaB b3946 0.33 -2.0
4,137,288 + fsaB b3946 0.33 -1.4
4,137,288 + fsaB b3946 0.33 -2.4
4,137,291 - fsaB b3946 0.33 -2.4
4,137,296 - fsaB b3946 0.34 +0.1
4,137,296 - fsaB b3946 0.34 -0.7
4,137,344 - fsaB b3946 0.41 +0.1
4,137,389 - fsaB b3946 0.48 +1.9
4,137,389 - fsaB b3946 0.48 -2.6
4,137,393 + fsaB b3946 0.49 +0.7
4,137,396 + fsaB b3946 0.49 +1.0
4,137,401 - fsaB b3946 0.50 -1.3
4,137,401 - fsaB b3946 0.50 -2.1
4,137,404 - fsaB b3946 0.51 +1.6
4,137,404 - fsaB b3946 0.51 -2.0
4,137,404 - fsaB b3946 0.51 -0.9
4,137,404 - fsaB b3946 0.51 +1.3
4,137,468 - fsaB b3946 0.60 +0.7
4,137,507 + fsaB b3946 0.66 -1.8
4,137,525 - fsaB b3946 0.69 +1.3
4,137,525 - fsaB b3946 0.69 +2.6
4,137,539 - fsaB b3946 0.71 +3.1
4,137,539 - fsaB b3946 0.71 -3.1
4,137,552 + fsaB b3946 0.73 +0.7
4,137,610 + fsaB b3946 0.82 +0.6
4,137,647 + fsaB b3946 0.87 +1.5
4,137,703 + +0.7
4,137,703 - +0.8
4,137,705 - +0.7
4,137,866 - +1.2
4,137,866 - -0.3
4,137,900 - -0.3
4,137,900 - -0.3
4,137,900 - -2.1
4,138,103 - ptsA b3947 0.14 +2.7
4,138,103 - ptsA b3947 0.14 -2.0
4,138,112 - ptsA b3947 0.15 +0.8
4,138,124 - ptsA b3947 0.15 -0.1
4,138,205 - ptsA b3947 0.18 -1.9
4,138,205 - ptsA b3947 0.18 +2.8
4,138,208 - ptsA b3947 0.19 +0.6
4,138,259 + ptsA b3947 0.21 -2.4
4,138,351 + ptsA b3947 0.24 +0.5
4,138,396 + ptsA b3947 0.26 +0.4
4,138,398 + ptsA b3947 0.26 -1.1
4,138,398 + ptsA b3947 0.26 +1.5
4,138,444 - ptsA b3947 0.28 -2.3
4,138,444 - ptsA b3947 0.28 -3.4
4,138,446 + ptsA b3947 0.28 +0.8
4,138,459 + ptsA b3947 0.29 +1.0
4,138,467 - ptsA b3947 0.29 -0.9
4,138,662 - ptsA b3947 0.37 +1.8
4,138,705 + ptsA b3947 0.38 +1.4
4,138,705 + ptsA b3947 0.38 +1.6

Or see this region's nucleotide sequence