Experiment: LB Anaerobic with Benzethonium chloride 0.00003125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt kefB and kefG overlap by 1 nucleotides kefG and yheS are separated by 127 nucleotides yheS and yheT overlap by 1 nucleotides
b3350: kefB - glutathione-regulated potassium-efflux system protein (NCBI), at 3,476,824 to 3,478,629
kefB
b3351: kefG - glutathione-regulated potassium-efflux system ancillary protein (NCBI), at 3,478,629 to 3,479,183
kefG
b3352: yheS - fused predicted transporter subunits of ABC superfamily: ATP-binding components (NCBI), at 3,479,311 to 3,481,224
yheS
b3353: yheT - predicted hydrolase (NCBI), at 3,481,224 to 3,482,246
yheT
Position (kb)
3479
3480
3481
3482 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3478.408 kb on - strand, within kefB at 3478.427 kb on - strand, within kefB at 3478.440 kb on - strand, within kefB at 3478.470 kb on - strand at 3478.494 kb on - strand at 3478.528 kb on + strand at 3478.547 kb on - strand at 3478.576 kb on + strand at 3478.578 kb on - strand at 3478.578 kb on - strand at 3478.637 kb on + strand at 3478.639 kb on + strand at 3478.639 kb on + strand at 3478.669 kb on + strand at 3478.680 kb on + strand at 3478.694 kb on + strand, within kefG at 3478.694 kb on + strand, within kefG at 3478.736 kb on + strand, within kefG at 3478.755 kb on + strand, within kefG at 3478.755 kb on + strand, within kefG at 3478.780 kb on + strand, within kefG at 3478.780 kb on + strand, within kefG at 3478.780 kb on - strand, within kefG at 3478.780 kb on - strand, within kefG at 3478.794 kb on + strand, within kefG at 3478.794 kb on + strand, within kefG at 3478.819 kb on + strand, within kefG at 3478.845 kb on + strand, within kefG at 3478.869 kb on - strand, within kefG at 3478.937 kb on - strand, within kefG at 3478.941 kb on + strand, within kefG at 3478.944 kb on + strand, within kefG at 3478.965 kb on + strand, within kefG at 3478.965 kb on + strand, within kefG at 3478.966 kb on + strand, within kefG at 3478.966 kb on + strand, within kefG at 3478.988 kb on + strand, within kefG at 3478.988 kb on + strand, within kefG at 3479.017 kb on - strand, within kefG at 3479.017 kb on - strand, within kefG at 3479.057 kb on + strand, within kefG at 3479.057 kb on + strand, within kefG at 3479.080 kb on + strand, within kefG at 3479.085 kb on + strand, within kefG at 3479.088 kb on - strand, within kefG at 3479.098 kb on + strand, within kefG at 3479.108 kb on + strand, within kefG at 3479.154 kb on - strand at 3479.170 kb on + strand at 3479.178 kb on - strand at 3479.178 kb on - strand at 3479.205 kb on + strand at 3479.205 kb on + strand at 3479.205 kb on + strand at 3479.305 kb on - strand at 3479.305 kb on - strand at 3479.437 kb on - strand at 3479.437 kb on - strand at 3479.445 kb on - strand at 3479.445 kb on - strand at 3479.486 kb on + strand at 3479.599 kb on + strand, within yheS at 3479.607 kb on - strand, within yheS at 3479.607 kb on - strand, within yheS at 3479.628 kb on - strand, within yheS at 3479.688 kb on - strand, within yheS at 3479.712 kb on - strand, within yheS at 3479.712 kb on - strand, within yheS at 3479.714 kb on + strand, within yheS at 3479.739 kb on - strand, within yheS at 3479.739 kb on - strand, within yheS at 3479.786 kb on + strand, within yheS at 3479.800 kb on - strand, within yheS at 3479.891 kb on + strand, within yheS at 3479.891 kb on + strand, within yheS at 3479.903 kb on + strand, within yheS at 3479.911 kb on - strand, within yheS at 3479.911 kb on - strand, within yheS at 3479.911 kb on - strand, within yheS at 3479.925 kb on - strand, within yheS at 3479.925 kb on - strand, within yheS at 3479.973 kb on + strand, within yheS at 3479.973 kb on + strand, within yheS at 3479.983 kb on + strand, within yheS at 3479.991 kb on - strand, within yheS at 3480.003 kb on - strand, within yheS at 3480.024 kb on - strand, within yheS at 3480.024 kb on - strand, within yheS at 3480.054 kb on + strand, within yheS at 3480.090 kb on + strand, within yheS at 3480.090 kb on + strand, within yheS at 3480.090 kb on - strand, within yheS at 3480.090 kb on - strand, within yheS at 3480.144 kb on - strand, within yheS at 3480.144 kb on - strand, within yheS at 3480.146 kb on + strand, within yheS at 3480.241 kb on - strand, within yheS at 3480.254 kb on - strand, within yheS at 3480.274 kb on - strand, within yheS at 3480.279 kb on - strand, within yheS at 3480.284 kb on + strand, within yheS at 3480.284 kb on + strand, within yheS at 3480.379 kb on - strand, within yheS at 3480.449 kb on - strand, within yheS at 3480.517 kb on - strand, within yheS at 3480.633 kb on + strand, within yheS at 3480.633 kb on + strand, within yheS at 3480.724 kb on + strand, within yheS at 3480.724 kb on + strand, within yheS at 3480.768 kb on - strand, within yheS at 3480.783 kb on + strand, within yheS at 3480.783 kb on + strand, within yheS at 3480.805 kb on - strand, within yheS at 3480.809 kb on - strand, within yheS at 3480.816 kb on - strand, within yheS at 3480.850 kb on - strand, within yheS at 3480.861 kb on - strand, within yheS at 3480.869 kb on - strand, within yheS at 3480.883 kb on - strand, within yheS at 3480.894 kb on + strand, within yheS at 3480.924 kb on - strand, within yheS at 3480.926 kb on + strand, within yheS at 3480.934 kb on - strand, within yheS at 3480.951 kb on + strand, within yheS at 3480.978 kb on + strand, within yheS at 3480.988 kb on - strand, within yheS at 3480.990 kb on + strand, within yheS at 3481.026 kb on - strand, within yheS at 3481.029 kb on + strand, within yheS at 3481.029 kb on + strand, within yheS at 3481.051 kb on + strand at 3481.078 kb on + strand at 3481.086 kb on - strand at 3481.086 kb on - strand at 3481.097 kb on - strand at 3481.102 kb on + strand at 3481.102 kb on + strand at 3481.102 kb on + strand at 3481.102 kb on + strand at 3481.208 kb on - strand at 3481.267 kb on + strand at 3481.274 kb on + strand at 3481.282 kb on - strand at 3481.316 kb on - strand at 3481.356 kb on + strand, within yheT at 3481.356 kb on - strand, within yheT at 3481.366 kb on - strand, within yheT at 3481.376 kb on - strand, within yheT at 3481.411 kb on - strand, within yheT at 3481.433 kb on + strand, within yheT at 3481.433 kb on + strand, within yheT at 3481.456 kb on - strand, within yheT at 3481.464 kb on + strand, within yheT at 3481.464 kb on + strand, within yheT at 3481.473 kb on + strand, within yheT at 3481.502 kb on + strand, within yheT at 3481.574 kb on + strand, within yheT at 3481.574 kb on - strand, within yheT at 3481.574 kb on - strand, within yheT at 3481.578 kb on + strand, within yheT at 3481.613 kb on + strand, within yheT at 3481.613 kb on + strand, within yheT at 3481.690 kb on + strand, within yheT at 3481.690 kb on + strand, within yheT at 3481.695 kb on - strand, within yheT at 3481.764 kb on - strand, within yheT at 3481.827 kb on + strand, within yheT at 3481.865 kb on + strand, within yheT at 3481.865 kb on + strand, within yheT at 3481.970 kb on + strand, within yheT at 3481.990 kb on + strand, within yheT at 3481.990 kb on + strand, within yheT at 3481.997 kb on + strand, within yheT at 3481.997 kb on + strand, within yheT at 3481.997 kb on + strand, within yheT at 3481.997 kb on + strand, within yheT at 3481.997 kb on + strand, within yheT at 3482.005 kb on - strand, within yheT at 3482.005 kb on - strand, within yheT at 3482.005 kb on - strand, within yheT at 3482.005 kb on - strand, within yheT at 3482.017 kb on - strand, within yheT at 3482.017 kb on - strand, within yheT at 3482.021 kb on - strand, within yheT at 3482.021 kb on - strand, within yheT at 3482.041 kb on + strand, within yheT at 3482.051 kb on + strand, within yheT at 3482.051 kb on + strand, within yheT at 3482.051 kb on + strand, within yheT at 3482.109 kb on - strand, within yheT at 3482.109 kb on - strand, within yheT at 3482.127 kb on - strand, within yheT at 3482.151 kb on - strand at 3482.181 kb on + strand at 3482.196 kb on - strand at 3482.196 kb on - strand at 3482.210 kb on - strand at 3482.210 kb on - strand at 3482.211 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Benzethonium chloride 0.00003125 mM remove 3,478,408 - kefB b3350 0.88 +0.4 3,478,427 - kefB b3350 0.89 +1.4 3,478,440 - kefB b3350 0.89 +0.8 3,478,470 - +2.1 3,478,494 - +1.1 3,478,528 + +2.4 3,478,547 - +0.6 3,478,576 + +0.2 3,478,578 - +1.3 3,478,578 - -2.7 3,478,637 + +2.3 3,478,639 + +1.4 3,478,639 + -1.0 3,478,669 + -2.5 3,478,680 + -0.1 3,478,694 + kefG b3351 0.12 -3.3 3,478,694 + kefG b3351 0.12 -1.9 3,478,736 + kefG b3351 0.19 -0.3 3,478,755 + kefG b3351 0.23 -1.8 3,478,755 + kefG b3351 0.23 +0.8 3,478,780 + kefG b3351 0.27 -1.9 3,478,780 + kefG b3351 0.27 +0.1 3,478,780 - kefG b3351 0.27 +2.6 3,478,780 - kefG b3351 0.27 -0.4 3,478,794 + kefG b3351 0.30 -2.3 3,478,794 + kefG b3351 0.30 +1.4 3,478,819 + kefG b3351 0.34 -1.5 3,478,845 + kefG b3351 0.39 -1.9 3,478,869 - kefG b3351 0.43 -2.9 3,478,937 - kefG b3351 0.55 -1.6 3,478,941 + kefG b3351 0.56 -0.8 3,478,944 + kefG b3351 0.57 +0.9 3,478,965 + kefG b3351 0.61 +0.3 3,478,965 + kefG b3351 0.61 -1.4 3,478,966 + kefG b3351 0.61 -2.9 3,478,966 + kefG b3351 0.61 -0.6 3,478,988 + kefG b3351 0.65 +2.6 3,478,988 + kefG b3351 0.65 -0.1 3,479,017 - kefG b3351 0.70 -2.6 3,479,017 - kefG b3351 0.70 -3.1 3,479,057 + kefG b3351 0.77 -2.3 3,479,057 + kefG b3351 0.77 -1.6 3,479,080 + kefG b3351 0.81 -0.6 3,479,085 + kefG b3351 0.82 -3.5 3,479,088 - kefG b3351 0.83 -0.6 3,479,098 + kefG b3351 0.85 -0.2 3,479,108 + kefG b3351 0.86 -1.9 3,479,154 - +0.0 3,479,170 + +0.4 3,479,178 - +1.1 3,479,178 - +1.7 3,479,205 + -2.2 3,479,205 + +1.0 3,479,205 + +1.7 3,479,305 - -0.9 3,479,305 - -0.1 3,479,437 - +2.2 3,479,437 - -0.9 3,479,445 - +2.2 3,479,445 - -0.7 3,479,486 + +1.6 3,479,599 + yheS b3352 0.15 +0.6 3,479,607 - yheS b3352 0.15 +0.5 3,479,607 - yheS b3352 0.15 -0.7 3,479,628 - yheS b3352 0.17 -0.6 3,479,688 - yheS b3352 0.20 -2.1 3,479,712 - yheS b3352 0.21 +1.8 3,479,712 - yheS b3352 0.21 -1.2 3,479,714 + yheS b3352 0.21 +2.3 3,479,739 - yheS b3352 0.22 -2.9 3,479,739 - yheS b3352 0.22 +1.5 3,479,786 + yheS b3352 0.25 +1.1 3,479,800 - yheS b3352 0.26 +1.6 3,479,891 + yheS b3352 0.30 +0.4 3,479,891 + yheS b3352 0.30 +0.6 3,479,903 + yheS b3352 0.31 +0.9 3,479,911 - yheS b3352 0.31 -1.4 3,479,911 - yheS b3352 0.31 +1.8 3,479,911 - yheS b3352 0.31 -0.9 3,479,925 - yheS b3352 0.32 +2.2 3,479,925 - yheS b3352 0.32 -2.6 3,479,973 + yheS b3352 0.35 +1.9 3,479,973 + yheS b3352 0.35 +1.4 3,479,983 + yheS b3352 0.35 +1.4 3,479,991 - yheS b3352 0.36 +1.5 3,480,003 - yheS b3352 0.36 +1.5 3,480,024 - yheS b3352 0.37 +1.3 3,480,024 - yheS b3352 0.37 +1.2 3,480,054 + yheS b3352 0.39 +0.7 3,480,090 + yheS b3352 0.41 +2.1 3,480,090 + yheS b3352 0.41 -1.6 3,480,090 - yheS b3352 0.41 +1.8 3,480,090 - yheS b3352 0.41 -2.3 3,480,144 - yheS b3352 0.44 -0.4 3,480,144 - yheS b3352 0.44 +1.5 3,480,146 + yheS b3352 0.44 +2.8 3,480,241 - yheS b3352 0.49 +1.3 3,480,254 - yheS b3352 0.49 +0.9 3,480,274 - yheS b3352 0.50 +1.7 3,480,279 - yheS b3352 0.51 -0.0 3,480,284 + yheS b3352 0.51 -2.1 3,480,284 + yheS b3352 0.51 -2.9 3,480,379 - yheS b3352 0.56 +0.6 3,480,449 - yheS b3352 0.59 +2.5 3,480,517 - yheS b3352 0.63 +1.9 3,480,633 + yheS b3352 0.69 +2.2 3,480,633 + yheS b3352 0.69 +3.0 3,480,724 + yheS b3352 0.74 +1.4 3,480,724 + yheS b3352 0.74 +0.3 3,480,768 - yheS b3352 0.76 +1.7 3,480,783 + yheS b3352 0.77 -0.1 3,480,783 + yheS b3352 0.77 +1.4 3,480,805 - yheS b3352 0.78 -1.6 3,480,809 - yheS b3352 0.78 -1.7 3,480,816 - yheS b3352 0.79 +0.9 3,480,850 - yheS b3352 0.80 +0.6 3,480,861 - yheS b3352 0.81 +0.6 3,480,869 - yheS b3352 0.81 -1.6 3,480,883 - yheS b3352 0.82 -3.3 3,480,894 + yheS b3352 0.83 +0.4 3,480,924 - yheS b3352 0.84 +1.5 3,480,926 + yheS b3352 0.84 -1.3 3,480,934 - yheS b3352 0.85 +0.8 3,480,951 + yheS b3352 0.86 +1.2 3,480,978 + yheS b3352 0.87 +2.1 3,480,988 - yheS b3352 0.88 -2.8 3,480,990 + yheS b3352 0.88 -3.3 3,481,026 - yheS b3352 0.90 +2.0 3,481,029 + yheS b3352 0.90 -2.2 3,481,029 + yheS b3352 0.90 +0.4 3,481,051 + +0.9 3,481,078 + -1.4 3,481,086 - -0.3 3,481,086 - +1.7 3,481,097 - -0.2 3,481,102 + -1.8 3,481,102 + +1.3 3,481,102 + -0.1 3,481,102 + +1.1 3,481,208 - +0.6 3,481,267 + -0.8 3,481,274 + +0.9 3,481,282 - -1.5 3,481,316 - -1.8 3,481,356 + yheT b3353 0.13 -3.2 3,481,356 - yheT b3353 0.13 -0.4 3,481,366 - yheT b3353 0.14 +1.4 3,481,376 - yheT b3353 0.15 +1.1 3,481,411 - yheT b3353 0.18 -1.6 3,481,433 + yheT b3353 0.20 -0.1 3,481,433 + yheT b3353 0.20 -2.2 3,481,456 - yheT b3353 0.23 +0.8 3,481,464 + yheT b3353 0.23 -2.2 3,481,464 + yheT b3353 0.23 -2.7 3,481,473 + yheT b3353 0.24 -1.9 3,481,502 + yheT b3353 0.27 +0.6 3,481,574 + yheT b3353 0.34 -1.1 3,481,574 - yheT b3353 0.34 -1.7 3,481,574 - yheT b3353 0.34 +0.5 3,481,578 + yheT b3353 0.35 +0.9 3,481,613 + yheT b3353 0.38 -2.1 3,481,613 + yheT b3353 0.38 +2.6 3,481,690 + yheT b3353 0.46 +3.0 3,481,690 + yheT b3353 0.46 +0.2 3,481,695 - yheT b3353 0.46 +1.0 3,481,764 - yheT b3353 0.53 +1.0 3,481,827 + yheT b3353 0.59 +0.6 3,481,865 + yheT b3353 0.63 +1.5 3,481,865 + yheT b3353 0.63 +0.9 3,481,970 + yheT b3353 0.73 +1.7 3,481,990 + yheT b3353 0.75 -0.8 3,481,990 + yheT b3353 0.75 +2.0 3,481,997 + yheT b3353 0.76 +1.4 3,481,997 + yheT b3353 0.76 +1.6 3,481,997 + yheT b3353 0.76 +1.6 3,481,997 + yheT b3353 0.76 +0.1 3,481,997 + yheT b3353 0.76 +0.2 3,482,005 - yheT b3353 0.76 +0.7 3,482,005 - yheT b3353 0.76 -0.9 3,482,005 - yheT b3353 0.76 +2.8 3,482,005 - yheT b3353 0.76 +0.5 3,482,017 - yheT b3353 0.78 +0.9 3,482,017 - yheT b3353 0.78 +0.3 3,482,021 - yheT b3353 0.78 +0.8 3,482,021 - yheT b3353 0.78 -2.9 3,482,041 + yheT b3353 0.80 -2.6 3,482,051 + yheT b3353 0.81 +1.0 3,482,051 + yheT b3353 0.81 -1.8 3,482,051 + yheT b3353 0.81 -2.7 3,482,109 - yheT b3353 0.87 -0.6 3,482,109 - yheT b3353 0.87 +2.8 3,482,127 - yheT b3353 0.88 -3.3 3,482,151 - +0.9 3,482,181 + -0.2 3,482,196 - +1.1 3,482,196 - -0.0 3,482,210 - -2.8 3,482,210 - -0.0 3,482,211 - -0.3
Or see this region's nucleotide sequence