Strain Fitness in Escherichia coli BW25113 around b2044

Experiment: LB Anaerobic with Benzethonium chloride 0.00003125 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntwcaM and wcaL are separated by 10 nucleotideswcaL and wcaK overlap by 4 nucleotides b2043: wcaM - predicted colanic acid biosynthesis protein (NCBI), at 2,112,526 to 2,113,920 wcaM b2044: wcaL - predicted glycosyl transferase (NCBI), at 2,113,931 to 2,115,151 wcaL b2045: wcaK - predicted pyruvyl transferase (NCBI), at 2,115,148 to 2,116,428 wcaK Position (kb) 2113 2114 2115 2116Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 2112.965 kb on - strand, within wcaMat 2112.965 kb on - strand, within wcaMat 2112.973 kb on - strand, within wcaMat 2113.022 kb on + strand, within wcaMat 2113.024 kb on + strand, within wcaMat 2113.050 kb on - strand, within wcaMat 2113.050 kb on - strand, within wcaMat 2113.060 kb on - strand, within wcaMat 2113.074 kb on - strand, within wcaMat 2113.125 kb on + strand, within wcaMat 2113.125 kb on + strand, within wcaMat 2113.188 kb on - strand, within wcaMat 2113.197 kb on - strand, within wcaMat 2113.207 kb on - strand, within wcaMat 2113.225 kb on - strand, within wcaMat 2113.233 kb on + strand, within wcaMat 2113.234 kb on + strand, within wcaMat 2113.234 kb on + strand, within wcaMat 2113.241 kb on - strand, within wcaMat 2113.242 kb on - strand, within wcaMat 2113.242 kb on - strand, within wcaMat 2113.263 kb on + strand, within wcaMat 2113.271 kb on + strand, within wcaMat 2113.274 kb on - strand, within wcaMat 2113.290 kb on + strand, within wcaMat 2113.290 kb on + strand, within wcaMat 2113.290 kb on + strand, within wcaMat 2113.338 kb on - strand, within wcaMat 2113.346 kb on - strand, within wcaMat 2113.357 kb on - strand, within wcaMat 2113.388 kb on - strand, within wcaMat 2113.414 kb on + strand, within wcaMat 2113.421 kb on - strand, within wcaMat 2113.421 kb on - strand, within wcaMat 2113.422 kb on - strand, within wcaMat 2113.435 kb on - strand, within wcaMat 2113.523 kb on - strand, within wcaMat 2113.530 kb on + strand, within wcaMat 2113.691 kb on + strand, within wcaMat 2113.691 kb on - strand, within wcaMat 2113.750 kb on - strand, within wcaMat 2113.750 kb on - strand, within wcaMat 2113.926 kb on + strandat 2114.118 kb on + strand, within wcaLat 2114.118 kb on + strand, within wcaLat 2114.251 kb on - strand, within wcaLat 2114.539 kb on - strand, within wcaLat 2114.789 kb on - strand, within wcaLat 2114.874 kb on - strand, within wcaLat 2115.177 kb on - strandat 2115.320 kb on + strand, within wcaKat 2115.414 kb on - strand, within wcaKat 2115.426 kb on - strand, within wcaKat 2115.426 kb on - strand, within wcaKat 2115.441 kb on + strand, within wcaKat 2115.507 kb on - strand, within wcaKat 2115.528 kb on + strand, within wcaKat 2115.548 kb on + strand, within wcaKat 2115.584 kb on + strand, within wcaKat 2115.585 kb on - strand, within wcaKat 2115.603 kb on - strand, within wcaKat 2115.651 kb on + strand, within wcaKat 2115.660 kb on + strand, within wcaKat 2115.708 kb on + strand, within wcaKat 2115.716 kb on - strand, within wcaKat 2115.754 kb on - strand, within wcaKat 2115.790 kb on + strand, within wcaKat 2115.941 kb on + strand, within wcaKat 2115.941 kb on + strand, within wcaKat 2115.949 kb on - strand, within wcaKat 2115.959 kb on - strand, within wcaKat 2115.959 kb on - strand, within wcaKat 2115.968 kb on + strand, within wcaKat 2115.968 kb on + strand, within wcaKat 2115.976 kb on - strand, within wcaKat 2115.995 kb on + strand, within wcaKat 2116.108 kb on + strand, within wcaKat 2116.108 kb on + strand, within wcaK

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Anaerobic with Benzethonium chloride 0.00003125 mM
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2,112,965 - wcaM b2043 0.31 -2.2
2,112,965 - wcaM b2043 0.31 -1.0
2,112,973 - wcaM b2043 0.32 +0.1
2,113,022 + wcaM b2043 0.36 -1.0
2,113,024 + wcaM b2043 0.36 -1.0
2,113,050 - wcaM b2043 0.38 -0.7
2,113,050 - wcaM b2043 0.38 -2.5
2,113,060 - wcaM b2043 0.38 -1.3
2,113,074 - wcaM b2043 0.39 +0.1
2,113,125 + wcaM b2043 0.43 -1.8
2,113,125 + wcaM b2043 0.43 -1.0
2,113,188 - wcaM b2043 0.47 -2.6
2,113,197 - wcaM b2043 0.48 +0.1
2,113,207 - wcaM b2043 0.49 -1.8
2,113,225 - wcaM b2043 0.50 -2.8
2,113,233 + wcaM b2043 0.51 +0.8
2,113,234 + wcaM b2043 0.51 +1.0
2,113,234 + wcaM b2043 0.51 +1.0
2,113,241 - wcaM b2043 0.51 +0.5
2,113,242 - wcaM b2043 0.51 +0.1
2,113,242 - wcaM b2043 0.51 +0.2
2,113,263 + wcaM b2043 0.53 +0.7
2,113,271 + wcaM b2043 0.53 +1.6
2,113,274 - wcaM b2043 0.54 +1.7
2,113,290 + wcaM b2043 0.55 +0.7
2,113,290 + wcaM b2043 0.55 +1.0
2,113,290 + wcaM b2043 0.55 -1.0
2,113,338 - wcaM b2043 0.58 +1.5
2,113,346 - wcaM b2043 0.59 -0.9
2,113,357 - wcaM b2043 0.60 -2.6
2,113,388 - wcaM b2043 0.62 +0.8
2,113,414 + wcaM b2043 0.64 -1.3
2,113,421 - wcaM b2043 0.64 -1.5
2,113,421 - wcaM b2043 0.64 +1.3
2,113,422 - wcaM b2043 0.64 +0.1
2,113,435 - wcaM b2043 0.65 -0.4
2,113,523 - wcaM b2043 0.71 +0.3
2,113,530 + wcaM b2043 0.72 +2.3
2,113,691 + wcaM b2043 0.84 +0.9
2,113,691 - wcaM b2043 0.84 -0.9
2,113,750 - wcaM b2043 0.88 -0.3
2,113,750 - wcaM b2043 0.88 +1.3
2,113,926 + +0.5
2,114,118 + wcaL b2044 0.15 -1.8
2,114,118 + wcaL b2044 0.15 -1.6
2,114,251 - wcaL b2044 0.26 +0.9
2,114,539 - wcaL b2044 0.50 -0.7
2,114,789 - wcaL b2044 0.70 -0.9
2,114,874 - wcaL b2044 0.77 +0.4
2,115,177 - -2.8
2,115,320 + wcaK b2045 0.13 -1.3
2,115,414 - wcaK b2045 0.21 -0.4
2,115,426 - wcaK b2045 0.22 +0.3
2,115,426 - wcaK b2045 0.22 -0.0
2,115,441 + wcaK b2045 0.23 +0.3
2,115,507 - wcaK b2045 0.28 -0.2
2,115,528 + wcaK b2045 0.30 +1.3
2,115,548 + wcaK b2045 0.31 -1.4
2,115,584 + wcaK b2045 0.34 +0.6
2,115,585 - wcaK b2045 0.34 +0.1
2,115,603 - wcaK b2045 0.36 +0.9
2,115,651 + wcaK b2045 0.39 -2.6
2,115,660 + wcaK b2045 0.40 -0.1
2,115,708 + wcaK b2045 0.44 +1.7
2,115,716 - wcaK b2045 0.44 -0.4
2,115,754 - wcaK b2045 0.47 -1.7
2,115,790 + wcaK b2045 0.50 +0.6
2,115,941 + wcaK b2045 0.62 -2.5
2,115,941 + wcaK b2045 0.62 +0.2
2,115,949 - wcaK b2045 0.63 -1.6
2,115,959 - wcaK b2045 0.63 -2.6
2,115,959 - wcaK b2045 0.63 +0.4
2,115,968 + wcaK b2045 0.64 +1.1
2,115,968 + wcaK b2045 0.64 +1.7
2,115,976 - wcaK b2045 0.65 -0.1
2,115,995 + wcaK b2045 0.66 +0.6
2,116,108 + wcaK b2045 0.75 +1.8
2,116,108 + wcaK b2045 0.75 -0.2

Or see this region's nucleotide sequence