Strain Fitness in Escherichia coli BW25113 around b1912

Experiment: LB Anaerobic with Benzethonium chloride 0.00003125 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyecA and leuZ are separated by 195 nucleotidesleuZ and cysT are separated by 12 nucleotidescysT and glyW are separated by 54 nucleotidesglyW and pgsA are separated by 151 nucleotidespgsA and uvrC are separated by 56 nucleotides b1908: yecA - conserved metal-binding protein (NCBI), at 1,988,978 to 1,989,643 yecA b1909: leuZ - tRNA-Leu (NCBI), at 1,989,839 to 1,989,925 leuZ b1910: cysT - tRNA-Cys (NCBI), at 1,989,938 to 1,990,011 cysT b1911: glyW - tRNA-Gly (NCBI), at 1,990,066 to 1,990,141 glyW b1912: pgsA - phosphatidylglycerophosphate synthetase (NCBI), at 1,990,293 to 1,990,841 pgsA b1913: uvrC - excinuclease ABC, subunit C; repair of UV damage to DNA (VIMSS), at 1,990,898 to 1,992,730 uvrC Position (kb) 1990 1991Strain fitness (log2 ratio) -2 -1 0 1 2at 1989.348 kb on + strand, within yecAat 1989.348 kb on + strand, within yecAat 1989.454 kb on + strand, within yecAat 1990.916 kb on + strandat 1991.220 kb on + strand, within uvrCat 1991.230 kb on + strand, within uvrCat 1991.528 kb on + strand, within uvrCat 1991.533 kb on + strand, within uvrCat 1991.533 kb on + strand, within uvrCat 1991.662 kb on + strand, within uvrCat 1991.662 kb on + strand, within uvrCat 1991.798 kb on - strand, within uvrCat 1991.798 kb on - strand, within uvrC

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Anaerobic with Benzethonium chloride 0.00003125 mM
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1,989,348 + yecA b1908 0.56 +0.9
1,989,348 + yecA b1908 0.56 -1.9
1,989,454 + yecA b1908 0.71 -1.4
1,990,916 + -2.1
1,991,220 + uvrC b1913 0.18 -1.7
1,991,230 + uvrC b1913 0.18 -1.6
1,991,528 + uvrC b1913 0.34 -0.2
1,991,533 + uvrC b1913 0.35 -1.4
1,991,533 + uvrC b1913 0.35 +2.3
1,991,662 + uvrC b1913 0.42 +1.4
1,991,662 + uvrC b1913 0.42 +0.4
1,991,798 - uvrC b1913 0.49 -0.8
1,991,798 - uvrC b1913 0.49 +2.0

Or see this region's nucleotide sequence