Strain Fitness in Escherichia coli BW25113 around b1491

Experiment: LB Anaerobic with Benzethonium chloride 0.00003125 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyddV and yddW are separated by 363 nucleotidesyddW and gadC are separated by 130 nucleotides b1490: yddV - predicted diguanylate cyclase (RefSeq), at 1,563,782 to 1,565,164 yddV b1491: yddW - predicted liprotein (NCBI), at 1,565,528 to 1,566,847 yddW b1492: gadC - predicted glutamate:gamma-aminobutyric acid antiporter (NCBI), at 1,566,978 to 1,568,513 gadC Position (kb) 1565 1566 1567Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 1564.872 kb on + strand, within yddVat 1564.876 kb on - strand, within yddVat 1564.965 kb on + strand, within yddVat 1565.116 kb on + strandat 1565.122 kb on + strandat 1565.295 kb on + strandat 1565.307 kb on - strandat 1565.313 kb on - strandat 1565.332 kb on - strandat 1565.460 kb on - strandat 1565.474 kb on - strandat 1565.474 kb on - strandat 1565.494 kb on - strandat 1565.551 kb on - strandat 1565.551 kb on - strandat 1565.580 kb on - strandat 1565.697 kb on + strand, within yddWat 1565.697 kb on + strand, within yddWat 1565.744 kb on - strand, within yddWat 1566.047 kb on + strand, within yddWat 1566.127 kb on - strand, within yddWat 1566.158 kb on + strand, within yddWat 1566.244 kb on - strand, within yddWat 1566.298 kb on + strand, within yddWat 1566.403 kb on - strand, within yddWat 1566.507 kb on - strand, within yddWat 1566.507 kb on - strand, within yddWat 1566.594 kb on - strand, within yddWat 1566.685 kb on + strand, within yddWat 1566.768 kb on - strandat 1566.778 kb on - strandat 1566.964 kb on + strandat 1566.979 kb on - strandat 1566.998 kb on + strandat 1567.129 kb on + strandat 1567.143 kb on + strand, within gadCat 1567.143 kb on + strand, within gadCat 1567.206 kb on - strand, within gadCat 1567.206 kb on - strand, within gadCat 1567.212 kb on - strand, within gadCat 1567.212 kb on - strand, within gadCat 1567.401 kb on - strand, within gadCat 1567.558 kb on + strand, within gadCat 1567.795 kb on - strand, within gadC

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Anaerobic with Benzethonium chloride 0.00003125 mM
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1,564,872 + yddV b1490 0.79 +1.0
1,564,876 - yddV b1490 0.79 +1.0
1,564,965 + yddV b1490 0.86 -0.5
1,565,116 + +0.3
1,565,122 + -1.7
1,565,295 + +1.0
1,565,307 - -1.3
1,565,313 - +1.0
1,565,332 - -1.3
1,565,460 - +1.0
1,565,474 - +0.0
1,565,474 - -1.3
1,565,494 - +1.0
1,565,551 - -0.5
1,565,551 - -0.5
1,565,580 - -2.9
1,565,697 + yddW b1491 0.13 -0.5
1,565,697 + yddW b1491 0.13 -3.0
1,565,744 - yddW b1491 0.16 -1.1
1,566,047 + yddW b1491 0.39 -2.8
1,566,127 - yddW b1491 0.45 -1.8
1,566,158 + yddW b1491 0.48 -0.3
1,566,244 - yddW b1491 0.54 -1.5
1,566,298 + yddW b1491 0.58 -0.5
1,566,403 - yddW b1491 0.66 +0.8
1,566,507 - yddW b1491 0.74 -1.9
1,566,507 - yddW b1491 0.74 -0.1
1,566,594 - yddW b1491 0.81 -3.0
1,566,685 + yddW b1491 0.88 -2.1
1,566,768 - -0.3
1,566,778 - -1.6
1,566,964 + +3.0
1,566,979 - +1.0
1,566,998 + -0.0
1,567,129 + +2.9
1,567,143 + gadC b1492 0.11 +0.0
1,567,143 + gadC b1492 0.11 +0.0
1,567,206 - gadC b1492 0.15 +0.9
1,567,206 - gadC b1492 0.15 +0.7
1,567,212 - gadC b1492 0.15 -1.3
1,567,212 - gadC b1492 0.15 +0.0
1,567,401 - gadC b1492 0.28 +0.6
1,567,558 + gadC b1492 0.38 -1.8
1,567,795 - gadC b1492 0.53 -1.8

Or see this region's nucleotide sequence