Strain Fitness in Escherichia coli BW25113 around b1185

Experiment: LB Anaerobic with Benzethonium chloride 0.00003125 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntumuC and dsbB are separated by 45 nucleotidesdsbB and nhaB are separated by 145 nucleotides b1184: umuC - DNA polymerase V subunit UmuC (NCBI), at 1,230,409 to 1,231,677 umuC b1185: dsbB - reoxidizes DsbA protein following formation of disulfide bond in P-ring of flagella. (VIMSS), at 1,231,723 to 1,232,253 dsbB b1186: nhaB - NhaB sodium/proton transporter (Katherine Huang), at 1,232,399 to 1,233,940 nhaB Position (kb) 1231 1232 1233Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 1230.893 kb on + strand, within umuCat 1230.956 kb on + strand, within umuCat 1231.013 kb on - strand, within umuCat 1231.029 kb on + strand, within umuCat 1231.038 kb on - strand, within umuCat 1231.109 kb on + strand, within umuCat 1231.109 kb on + strand, within umuCat 1231.208 kb on + strandat 1231.208 kb on + strand, within umuCat 1231.270 kb on + strand, within umuCat 1231.270 kb on + strand, within umuCat 1231.320 kb on + strand, within umuCat 1231.359 kb on + strand, within umuCat 1231.359 kb on + strand, within umuCat 1231.382 kb on - strand, within umuCat 1231.457 kb on + strand, within umuCat 1231.519 kb on - strand, within umuCat 1231.550 kb on + strand, within umuCat 1231.551 kb on - strandat 1231.553 kb on - strandat 1231.573 kb on - strandat 1231.597 kb on + strandat 1231.625 kb on + strandat 1231.627 kb on - strandat 1231.643 kb on - strandat 1231.645 kb on - strandat 1231.660 kb on - strandat 1231.961 kb on + strand, within dsbBat 1231.961 kb on + strand, within dsbBat 1231.979 kb on - strand, within dsbBat 1232.074 kb on + strand, within dsbBat 1232.074 kb on + strand, within dsbBat 1232.077 kb on - strand, within dsbBat 1232.272 kb on + strandat 1232.272 kb on + strandat 1232.504 kb on - strandat 1232.509 kb on - strandat 1232.525 kb on + strandat 1232.569 kb on - strand, within nhaBat 1232.784 kb on - strand, within nhaBat 1232.784 kb on - strand, within nhaBat 1232.921 kb on - strand, within nhaBat 1232.921 kb on - strand, within nhaBat 1232.936 kb on + strand, within nhaBat 1233.026 kb on + strand, within nhaBat 1233.087 kb on - strand, within nhaBat 1233.196 kb on - strand, within nhaB

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Anaerobic with Benzethonium chloride 0.00003125 mM
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1,230,893 + umuC b1184 0.38 +1.4
1,230,956 + umuC b1184 0.43 -1.4
1,231,013 - umuC b1184 0.48 +0.3
1,231,029 + umuC b1184 0.49 -0.4
1,231,038 - umuC b1184 0.50 +0.6
1,231,109 + umuC b1184 0.55 -1.9
1,231,109 + umuC b1184 0.55 +0.6
1,231,208 + +2.5
1,231,208 + umuC b1184 0.63 -3.8
1,231,270 + umuC b1184 0.68 +0.0
1,231,270 + umuC b1184 0.68 -2.3
1,231,320 + umuC b1184 0.72 -0.3
1,231,359 + umuC b1184 0.75 -1.7
1,231,359 + umuC b1184 0.75 +1.0
1,231,382 - umuC b1184 0.77 +1.3
1,231,457 + umuC b1184 0.83 +1.6
1,231,519 - umuC b1184 0.87 +0.7
1,231,550 + umuC b1184 0.90 -1.1
1,231,551 - +0.3
1,231,553 - -0.7
1,231,573 - +0.3
1,231,597 + +1.3
1,231,625 + +0.2
1,231,627 - +0.9
1,231,643 - +0.9
1,231,645 - -1.4
1,231,660 - +0.9
1,231,961 + dsbB b1185 0.45 -1.3
1,231,961 + dsbB b1185 0.45 -0.7
1,231,979 - dsbB b1185 0.48 -3.1
1,232,074 + dsbB b1185 0.66 -1.1
1,232,074 + dsbB b1185 0.66 -1.4
1,232,077 - dsbB b1185 0.67 -0.7
1,232,272 + -0.5
1,232,272 + -0.2
1,232,504 - +0.5
1,232,509 - -0.5
1,232,525 + -0.2
1,232,569 - nhaB b1186 0.11 -1.1
1,232,784 - nhaB b1186 0.25 +0.9
1,232,784 - nhaB b1186 0.25 -2.6
1,232,921 - nhaB b1186 0.34 -0.7
1,232,921 - nhaB b1186 0.34 +0.1
1,232,936 + nhaB b1186 0.35 +0.2
1,233,026 + nhaB b1186 0.41 -0.3
1,233,087 - nhaB b1186 0.45 +0.5
1,233,196 - nhaB b1186 0.52 -0.1

Or see this region's nucleotide sequence