Strain Fitness in Escherichia coli BW25113 around b0929

Experiment: LB Anaerobic with Benzethonium chloride 0.00003125 mM

Add experiment(s):

Zoom: Pan:

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

download SVG
500 ntaspC and ompF are separated by 184 nucleotidesompF and asnS are separated by 602 nucleotides b0928: aspC - aspartate aminotransferase, PLP-dependent (NCBI), at 983,742 to 984,932 aspC b0929: ompF - outer membrane porin 1a (Ia;b;F) (NCBI), at 985,117 to 986,205 ompF b0930: asnS - asparaginyl-tRNA synthetase (NCBI), at 986,808 to 988,208 asnS Position (kb) 985 986 987Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 984.232 kb on - strand, within aspCat 984.326 kb on + strand, within aspCat 984.365 kb on - strand, within aspCat 984.402 kb on + strand, within aspCat 984.402 kb on + strand, within aspCat 984.535 kb on - strand, within aspCat 984.560 kb on + strand, within aspCat 984.560 kb on + strand, within aspCat 984.633 kb on + strand, within aspCat 984.633 kb on + strand, within aspCat 984.683 kb on - strand, within aspCat 984.792 kb on + strand, within aspCat 984.939 kb on + strandat 984.942 kb on + strandat 984.944 kb on + strandat 984.975 kb on + strandat 985.123 kb on - strandat 985.169 kb on + strandat 985.240 kb on + strand, within ompFat 985.259 kb on - strand, within ompFat 985.483 kb on + strand, within ompFat 985.548 kb on - strand, within ompFat 985.548 kb on - strand, within ompFat 985.682 kb on + strand, within ompFat 985.682 kb on + strand, within ompFat 985.805 kb on + strand, within ompFat 985.813 kb on - strand, within ompFat 985.880 kb on - strand, within ompFat 985.934 kb on + strand, within ompFat 985.934 kb on + strand, within ompFat 986.017 kb on + strand, within ompFat 986.017 kb on + strand, within ompFat 986.164 kb on - strandat 986.164 kb on - strandat 986.179 kb on - strandat 986.217 kb on - strandat 986.217 kb on - strandat 986.445 kb on - strand

download strain data

Per-strain Table

Position Strand Gene LocusTag Fraction LB Anaerobic with Benzethonium chloride 0.00003125 mM
remove
984,232 - aspC b0928 0.41 +0.9
984,326 + aspC b0928 0.49 +1.1
984,365 - aspC b0928 0.52 +2.6
984,402 + aspC b0928 0.55 -1.4
984,402 + aspC b0928 0.55 +1.5
984,535 - aspC b0928 0.67 -2.3
984,560 + aspC b0928 0.69 -1.5
984,560 + aspC b0928 0.69 -0.3
984,633 + aspC b0928 0.75 +1.0
984,633 + aspC b0928 0.75 +1.4
984,683 - aspC b0928 0.79 +0.4
984,792 + aspC b0928 0.88 +1.9
984,939 + +0.6
984,942 + -0.5
984,944 + +2.1
984,975 + -1.0
985,123 - +0.2
985,169 + +1.0
985,240 + ompF b0929 0.11 +0.6
985,259 - ompF b0929 0.13 -2.2
985,483 + ompF b0929 0.34 +0.0
985,548 - ompF b0929 0.40 -1.3
985,548 - ompF b0929 0.40 -0.6
985,682 + ompF b0929 0.52 +1.0
985,682 + ompF b0929 0.52 -1.0
985,805 + ompF b0929 0.63 -1.3
985,813 - ompF b0929 0.64 -3.8
985,880 - ompF b0929 0.70 -0.5
985,934 + ompF b0929 0.75 -2.3
985,934 + ompF b0929 0.75 -1.8
986,017 + ompF b0929 0.83 -1.8
986,017 + ompF b0929 0.83 +1.6
986,164 - -3.7
986,164 - -2.1
986,179 - -1.8
986,217 - -3.7
986,217 - -1.2
986,445 - -0.4

Or see this region's nucleotide sequence