Experiment: LB Anaerobic with 8-hydroxy-nitroquinoline 0.0000025 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ylbH and ybbD overlap by 20 nucleotides ybbD and ylbI are separated by 369 nucleotides ylbI and ylbG are separated by 2 nucleotides ylbG and ybbB are separated by 115 nucleotides
b0499: ylbH - conserved protein, rhs-like (RefSeq), at 527,176 to 527,883
ylbH
b0500: ybbD - hypothetical protein (RefSeq), at 527,864 to 528,354
ybbD
b4632: ylbI - no description, at 528,724 to 528,816
ylbI
b0502: ylbG - no description, at 528,819 to 529,240
ylbG
b0503: ybbB - tRNA 2-selenouridine synthase, selenophosphate-dependent (NCBI), at 529,356 to 530,450
ybbB
Position (kb) 528
529
530 Strain fitness (log2 ratio) -2
-1
0
1 at 528.145 kb on - strand, within ybbD at 528.146 kb on - strand, within ybbD at 528.146 kb on - strand, within ybbD at 528.328 kb on + strand at 528.377 kb on - strand at 528.384 kb on + strand at 528.446 kb on + strand at 528.454 kb on + strand at 528.502 kb on + strand at 528.502 kb on + strand at 528.512 kb on + strand at 528.750 kb on - strand, within ylbI at 528.757 kb on + strand, within ylbI at 528.757 kb on + strand, within ylbI at 528.759 kb on + strand, within ylbI at 528.765 kb on - strand, within ylbI at 528.765 kb on - strand, within ylbI at 528.765 kb on - strand, within ylbI at 528.765 kb on - strand, within ylbI at 528.765 kb on - strand, within ylbI at 528.767 kb on - strand, within ylbI at 528.770 kb on - strand, within ylbI at 528.770 kb on - strand, within ylbI at 528.770 kb on - strand, within ylbI at 528.777 kb on + strand, within ylbI at 528.777 kb on + strand, within ylbI at 528.786 kb on - strand, within ylbI at 528.786 kb on - strand, within ylbI at 528.786 kb on - strand, within ylbI at 528.786 kb on - strand, within ylbI at 528.793 kb on - strand, within ylbI at 528.799 kb on - strand, within ylbI at 528.803 kb on - strand, within ylbI at 528.803 kb on - strand, within ylbI at 528.834 kb on - strand at 528.834 kb on - strand at 528.880 kb on + strand, within ylbG at 528.897 kb on - strand, within ylbG at 528.897 kb on - strand, within ylbG at 528.969 kb on + strand, within ylbG at 529.243 kb on + strand at 529.243 kb on + strand at 529.243 kb on + strand at 529.249 kb on - strand at 529.269 kb on + strand at 529.312 kb on - strand at 529.437 kb on - strand at 529.728 kb on - strand, within ybbB at 529.728 kb on - strand, within ybbB at 529.906 kb on - strand, within ybbB at 529.911 kb on + strand, within ybbB at 529.911 kb on + strand, within ybbB at 529.959 kb on + strand, within ybbB at 529.959 kb on + strand, within ybbB at 530.005 kb on - strand, within ybbB at 530.209 kb on + strand, within ybbB
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with 8-hydroxy-nitroquinoline 0.0000025 mM remove 528,145 - ybbD b0500 0.57 +0.7 528,146 - ybbD b0500 0.57 +0.7 528,146 - ybbD b0500 0.57 +0.7 528,328 + +0.7 528,377 - +0.7 528,384 + +0.7 528,446 + +0.7 528,454 + +0.7 528,502 + +0.7 528,502 + +0.7 528,512 + +0.7 528,750 - ylbI b4632 0.28 -0.5 528,757 + ylbI b4632 0.35 +0.7 528,757 + ylbI b4632 0.35 +0.7 528,759 + ylbI b4632 0.38 -0.3 528,765 - ylbI b4632 0.44 -1.3 528,765 - ylbI b4632 0.44 -0.4 528,765 - ylbI b4632 0.44 -0.1 528,765 - ylbI b4632 0.44 -0.3 528,765 - ylbI b4632 0.44 -0.2 528,767 - ylbI b4632 0.46 -0.1 528,770 - ylbI b4632 0.49 +1.0 528,770 - ylbI b4632 0.49 +1.2 528,770 - ylbI b4632 0.49 -0.1 528,777 + ylbI b4632 0.57 -1.9 528,777 + ylbI b4632 0.57 -1.9 528,786 - ylbI b4632 0.67 -0.3 528,786 - ylbI b4632 0.67 -0.9 528,786 - ylbI b4632 0.67 +0.0 528,786 - ylbI b4632 0.67 -1.0 528,793 - ylbI b4632 0.74 -1.1 528,799 - ylbI b4632 0.81 +0.2 528,803 - ylbI b4632 0.85 -0.5 528,803 - ylbI b4632 0.85 -0.3 528,834 - +1.1 528,834 - +0.4 528,880 + ylbG b0502 0.14 +0.3 528,897 - ylbG b0502 0.18 +1.1 528,897 - ylbG b0502 0.18 +0.7 528,969 + ylbG b0502 0.36 -0.9 529,243 + -0.5 529,243 + -0.3 529,243 + -0.0 529,249 - -1.8 529,269 + -0.3 529,312 - +0.3 529,437 - -0.6 529,728 - ybbB b0503 0.34 -0.2 529,728 - ybbB b0503 0.34 +1.6 529,906 - ybbB b0503 0.50 -0.2 529,911 + ybbB b0503 0.51 -1.6 529,911 + ybbB b0503 0.51 +0.5 529,959 + ybbB b0503 0.55 -0.3 529,959 + ybbB b0503 0.55 -1.2 530,005 - ybbB b0503 0.59 -0.5 530,209 + ybbB b0503 0.78 -0.1
Or see this region's nucleotide sequence