Strain Fitness in Escherichia coli BW25113 around b4588

Experiment: LB Anaerobic with 8-hydroxy-nitroquinoline 0.0000025 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntybcL and ybcM are separated by 9 nucleotidesybcM and ylcH are separated by 116 nucleotidesylcH and ybcN overlap by 4 nucleotidesybcN and ninE overlap by 1 nucleotidesninE and ybcO overlap by 8 nucleotidesybcO and rusA overlap by 4 nucleotides b0545: ybcL - DLP12 prophage; predicted kinase inhibitor (NCBI), at 570,116 to 570,667 ybcL b0546: ybcM - DLP12 prophage; predicted DNA-binding transcriptional regulator (NCBI), at 570,677 to 571,474 ybcM b4588: ylcH - hypothetical protein, DLP12 prophage (RefSeq), at 571,591 to 571,692 ylcH b0547: ybcN - DLP12 prophage; predicted protein (NCBI), at 571,689 to 572,144 ybcN b0548: ninE - DLP12 prophage; conserved protein (NCBI), at 572,144 to 572,314 ninE b0549: ybcO - DLP12 prophage; predicted protein (NCBI), at 572,307 to 572,597 ybcO b0550: rusA - DLP12 prophage; endonuclease RUS (NCBI), at 572,594 to 572,956 rusA Position (kb) 571 572Strain fitness (log2 ratio) -2 -1 0 1 2at 570.625 kb on + strandat 570.644 kb on + strandat 570.650 kb on + strandat 570.650 kb on + strandat 570.661 kb on + strandat 570.665 kb on - strandat 570.668 kb on + strandat 570.668 kb on + strandat 570.672 kb on + strandat 570.675 kb on - strandat 570.675 kb on - strandat 570.675 kb on - strandat 570.682 kb on + strandat 570.682 kb on + strandat 570.688 kb on + strandat 570.688 kb on + strandat 570.697 kb on - strandat 570.772 kb on + strand, within ybcMat 570.772 kb on + strand, within ybcMat 570.804 kb on + strand, within ybcMat 570.804 kb on + strand, within ybcMat 570.804 kb on + strand, within ybcMat 570.804 kb on + strand, within ybcMat 570.804 kb on + strand, within ybcMat 570.817 kb on + strand, within ybcMat 570.896 kb on + strand, within ybcMat 570.992 kb on + strand, within ybcMat 570.992 kb on + strand, within ybcMat 571.055 kb on + strand, within ybcMat 571.084 kb on - strand, within ybcMat 571.094 kb on - strand, within ybcMat 571.105 kb on + strand, within ybcMat 571.115 kb on + strand, within ybcMat 571.124 kb on - strand, within ybcMat 571.130 kb on - strand, within ybcMat 571.170 kb on - strand, within ybcMat 571.186 kb on + strand, within ybcMat 571.207 kb on + strand, within ybcMat 571.207 kb on + strand, within ybcMat 571.215 kb on - strand, within ybcMat 571.226 kb on + strand, within ybcMat 571.283 kb on + strand, within ybcMat 571.301 kb on + strand, within ybcMat 571.301 kb on + strand, within ybcMat 571.306 kb on + strand, within ybcMat 571.340 kb on + strand, within ybcMat 571.340 kb on + strand, within ybcMat 571.340 kb on + strand, within ybcMat 571.340 kb on + strand, within ybcMat 571.341 kb on - strand, within ybcMat 571.345 kb on - strand, within ybcMat 571.358 kb on - strand, within ybcMat 571.418 kb on + strandat 571.427 kb on + strandat 571.477 kb on - strandat 571.486 kb on - strandat 571.546 kb on - strandat 571.560 kb on - strandat 571.575 kb on - strandat 571.575 kb on - strandat 571.585 kb on - strandat 571.596 kb on + strandat 571.596 kb on - strandat 571.596 kb on - strandat 571.602 kb on - strand, within ylcHat 571.618 kb on + strand, within ylcHat 571.623 kb on - strand, within ylcHat 571.629 kb on + strand, within ylcHat 571.638 kb on - strand, within ylcHat 571.671 kb on + strand, within ylcHat 571.681 kb on + strand, within ylcHat 571.681 kb on + strand, within ylcHat 571.682 kb on - strandat 571.682 kb on - strandat 571.682 kb on - strandat 571.682 kb on - strandat 571.682 kb on - strandat 571.682 kb on - strandat 571.682 kb on - strandat 571.687 kb on + strandat 571.694 kb on - strandat 571.695 kb on - strandat 571.752 kb on + strand, within ybcNat 571.752 kb on + strand, within ybcNat 571.909 kb on + strand, within ybcNat 571.931 kb on + strand, within ybcNat 571.937 kb on - strand, within ybcNat 571.939 kb on - strand, within ybcNat 571.939 kb on - strand, within ybcNat 572.055 kb on + strand, within ybcNat 572.068 kb on - strand, within ybcNat 572.179 kb on + strand, within ninEat 572.179 kb on + strand, within ninEat 572.179 kb on + strand, within ninEat 572.195 kb on - strand, within ninEat 572.214 kb on - strand, within ninEat 572.343 kb on - strand, within ybcOat 572.557 kb on - strand, within ybcOat 572.617 kb on + strandat 572.625 kb on - strandat 572.679 kb on - strand, within rusAat 572.679 kb on - strand, within rusA

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Anaerobic with 8-hydroxy-nitroquinoline 0.0000025 mM
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570,625 + +0.1
570,644 + -0.3
570,650 + -0.3
570,650 + -0.0
570,661 + +0.0
570,665 - +0.7
570,668 + -0.3
570,668 + -0.3
570,672 + +0.3
570,675 - +0.7
570,675 - +0.7
570,675 - +0.7
570,682 + +0.7
570,682 + +1.7
570,688 + +0.7
570,688 + +0.7
570,697 - +0.7
570,772 + ybcM b0546 0.12 -0.7
570,772 + ybcM b0546 0.12 -0.4
570,804 + ybcM b0546 0.16 +0.4
570,804 + ybcM b0546 0.16 -0.7
570,804 + ybcM b0546 0.16 -0.6
570,804 + ybcM b0546 0.16 +0.5
570,804 + ybcM b0546 0.16 -0.9
570,817 + ybcM b0546 0.18 -0.4
570,896 + ybcM b0546 0.27 +0.7
570,992 + ybcM b0546 0.39 +0.7
570,992 + ybcM b0546 0.39 -0.3
571,055 + ybcM b0546 0.47 +0.7
571,084 - ybcM b0546 0.51 +0.7
571,094 - ybcM b0546 0.52 +0.7
571,105 + ybcM b0546 0.54 +0.7
571,115 + ybcM b0546 0.55 +0.7
571,124 - ybcM b0546 0.56 +0.7
571,130 - ybcM b0546 0.57 +0.7
571,170 - ybcM b0546 0.62 +0.7
571,186 + ybcM b0546 0.64 -0.3
571,207 + ybcM b0546 0.66 +0.7
571,207 + ybcM b0546 0.66 +0.7
571,215 - ybcM b0546 0.67 +0.7
571,226 + ybcM b0546 0.69 -0.9
571,283 + ybcM b0546 0.76 -0.5
571,301 + ybcM b0546 0.78 -0.3
571,301 + ybcM b0546 0.78 +0.7
571,306 + ybcM b0546 0.79 -2.1
571,340 + ybcM b0546 0.83 +0.7
571,340 + ybcM b0546 0.83 -0.3
571,340 + ybcM b0546 0.83 -0.3
571,340 + ybcM b0546 0.83 -0.9
571,341 - ybcM b0546 0.83 +0.7
571,345 - ybcM b0546 0.84 +0.7
571,358 - ybcM b0546 0.85 +0.7
571,418 + +0.9
571,427 + -0.3
571,477 - +0.7
571,486 - +0.7
571,546 - -0.3
571,560 - +0.1
571,575 - +0.7
571,575 - -1.6
571,585 - +0.1
571,596 + +0.7
571,596 - +0.3
571,596 - -0.3
571,602 - ylcH b4588 0.11 +0.7
571,618 + ylcH b4588 0.26 +0.7
571,623 - ylcH b4588 0.31 -0.5
571,629 + ylcH b4588 0.37 -0.5
571,638 - ylcH b4588 0.46 +0.6
571,671 + ylcH b4588 0.78 +1.0
571,681 + ylcH b4588 0.88 -1.3
571,681 + ylcH b4588 0.88 +1.5
571,682 - -0.8
571,682 - +0.3
571,682 - +0.7
571,682 - -0.3
571,682 - -0.0
571,682 - +0.2
571,682 - -0.3
571,687 + -0.3
571,694 - +1.3
571,695 - -1.3
571,752 + ybcN b0547 0.14 +0.3
571,752 + ybcN b0547 0.14 -0.3
571,909 + ybcN b0547 0.48 -0.6
571,931 + ybcN b0547 0.53 +0.1
571,937 - ybcN b0547 0.54 -0.2
571,939 - ybcN b0547 0.55 +0.8
571,939 - ybcN b0547 0.55 +0.3
572,055 + ybcN b0547 0.80 -0.1
572,068 - ybcN b0547 0.83 -0.1
572,179 + ninE b0548 0.20 -0.7
572,179 + ninE b0548 0.20 -0.9
572,179 + ninE b0548 0.20 -0.7
572,195 - ninE b0548 0.30 -0.2
572,214 - ninE b0548 0.41 +0.2
572,343 - ybcO b0549 0.12 +0.7
572,557 - ybcO b0549 0.86 -0.4
572,617 + -1.1
572,625 - +0.6
572,679 - rusA b0550 0.23 +2.0
572,679 - rusA b0550 0.23 +0.1

Or see this region's nucleotide sequence