Strain Fitness in Escherichia coli BW25113 around b1647

Experiment: LB Anaerobic with 8-hydroxy-nitroquinoline 0.0000025 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntydhK and sodC are separated by 0 nucleotidessodC and ydhF are separated by 80 nucleotidesydhF and b1648 are separated by 48 nucleotidesb1648 and ydhM are separated by 102 nucleotides b1645: ydhK - conserved inner membrane protein (NCBI), at 1,720,145 to 1,722,157 ydhK b1646: sodC - superoxide dismutase, Cu, Zn (NCBI), at 1,722,158 to 1,722,679 sodC b1647: ydhF - predicted oxidoreductase (NCBI), at 1,722,760 to 1,723,656 ydhF b1648: b1648 - orf, hypothetical protein (VIMSS), at 1,723,705 to 1,723,944 b1648 b1649: ydhM - predicted DNA-binding transcriptional regulator (RefSeq), at 1,724,047 to 1,724,646 ydhM Position (kb) 1722 1723 1724Strain fitness (log2 ratio) -2 -1 0 1 2at 1721.762 kb on - strand, within ydhKat 1721.805 kb on - strand, within ydhKat 1721.805 kb on - strand, within ydhKat 1721.877 kb on - strand, within ydhKat 1721.877 kb on - strand, within ydhKat 1721.941 kb on + strand, within ydhKat 1722.103 kb on - strandat 1722.176 kb on + strandat 1722.692 kb on - strandat 1722.692 kb on - strandat 1722.868 kb on - strand, within ydhFat 1722.868 kb on - strand, within ydhFat 1722.904 kb on - strand, within ydhFat 1722.904 kb on - strand, within ydhFat 1723.132 kb on + strand, within ydhFat 1723.138 kb on - strand, within ydhFat 1723.490 kb on + strand, within ydhFat 1723.797 kb on - strand, within b1648at 1724.028 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Anaerobic with 8-hydroxy-nitroquinoline 0.0000025 mM
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1,721,762 - ydhK b1645 0.80 +1.0
1,721,805 - ydhK b1645 0.82 -0.3
1,721,805 - ydhK b1645 0.82 +0.6
1,721,877 - ydhK b1645 0.86 -2.4
1,721,877 - ydhK b1645 0.86 -1.1
1,721,941 + ydhK b1645 0.89 +0.4
1,722,103 - -0.4
1,722,176 + +0.8
1,722,692 - -0.6
1,722,692 - -0.8
1,722,868 - ydhF b1647 0.12 +2.2
1,722,868 - ydhF b1647 0.12 +1.1
1,722,904 - ydhF b1647 0.16 -0.0
1,722,904 - ydhF b1647 0.16 +0.3
1,723,132 + ydhF b1647 0.41 -0.4
1,723,138 - ydhF b1647 0.42 +1.4
1,723,490 + ydhF b1647 0.81 +1.4
1,723,797 - b1648 b1648 0.38 -0.4
1,724,028 + -0.0

Or see this region's nucleotide sequence