Experiment: LB Anaerobic with 8-hydroxy-nitroquinoline 0.0000025 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt stfQ and nohA overlap by 8 nucleotides nohA and ynfO are separated by 388 nucleotides ynfO and ydfO are separated by 57 nucleotides ydfO and b1550 are separated by 151 nucleotides b1550 and b1551 are separated by 171 nucleotides
b1547: stfQ - Qin prophage; predicted side tail fibre assembly protein (NCBI), at 1,632,909 to 1,633,871
stfQ
b1548: nohA - Qin prophage; predicted packaging protein (RefSeq), at 1,633,864 to 1,634,391
nohA
b4533: ynfO - Qin prophage; predicted protein (NCBI), at 1,634,780 to 1,635,013
ynfO
b1549: ydfO - orf, hypothetical protein (VIMSS), at 1,635,071 to 1,635,481
ydfO
b1550: b1550 - orf, hypothetical protein (VIMSS), at 1,635,633 to 1,635,806
b1550
b1551: b1551 - orf, hypothetical protein (VIMSS), at 1,635,978 to 1,636,133
b1551
Position (kb)
1634
1635
1636 Strain fitness (log2 ratio)
-1
0
1
2 at 1633.829 kb on - strand at 1634.153 kb on + strand, within nohA at 1634.153 kb on + strand, within nohA at 1634.884 kb on + strand, within ynfO at 1634.884 kb on + strand, within ynfO at 1634.916 kb on + strand, within ynfO at 1634.916 kb on + strand, within ynfO at 1635.711 kb on - strand, within b1550 at 1635.711 kb on - strand, within b1550 at 1635.823 kb on - strand at 1635.823 kb on - strand at 1635.847 kb on - strand at 1635.847 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with 8-hydroxy-nitroquinoline 0.0000025 mM remove 1,633,829 - +1.4 1,634,153 + nohA b1548 0.55 +0.9 1,634,153 + nohA b1548 0.55 -0.9 1,634,884 + ynfO b4533 0.44 -0.6 1,634,884 + ynfO b4533 0.44 -0.1 1,634,916 + ynfO b4533 0.58 +2.0 1,634,916 + ynfO b4533 0.58 +1.1 1,635,711 - b1550 b1550 0.45 -0.3 1,635,711 - b1550 b1550 0.45 +0.4 1,635,823 - -0.6 1,635,823 - +0.2 1,635,847 - -0.7 1,635,847 - -0.5
Or see this region's nucleotide sequence