Strain Fitness in Escherichia coli BW25113 around b4533

Experiment: LB Anaerobic with 8-hydroxy-nitroquinoline 0.0000025 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntstfQ and nohA overlap by 8 nucleotidesnohA and ynfO are separated by 388 nucleotidesynfO and ydfO are separated by 57 nucleotidesydfO and b1550 are separated by 151 nucleotidesb1550 and b1551 are separated by 171 nucleotides b1547: stfQ - Qin prophage; predicted side tail fibre assembly protein (NCBI), at 1,632,909 to 1,633,871 stfQ b1548: nohA - Qin prophage; predicted packaging protein (RefSeq), at 1,633,864 to 1,634,391 nohA b4533: ynfO - Qin prophage; predicted protein (NCBI), at 1,634,780 to 1,635,013 ynfO b1549: ydfO - orf, hypothetical protein (VIMSS), at 1,635,071 to 1,635,481 ydfO b1550: b1550 - orf, hypothetical protein (VIMSS), at 1,635,633 to 1,635,806 b1550 b1551: b1551 - orf, hypothetical protein (VIMSS), at 1,635,978 to 1,636,133 b1551 Position (kb) 1634 1635 1636Strain fitness (log2 ratio) -1 0 1 2at 1633.829 kb on - strandat 1634.153 kb on + strand, within nohAat 1634.153 kb on + strand, within nohAat 1634.884 kb on + strand, within ynfOat 1634.884 kb on + strand, within ynfOat 1634.916 kb on + strand, within ynfOat 1634.916 kb on + strand, within ynfOat 1635.711 kb on - strand, within b1550at 1635.711 kb on - strand, within b1550at 1635.823 kb on - strandat 1635.823 kb on - strandat 1635.847 kb on - strandat 1635.847 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Anaerobic with 8-hydroxy-nitroquinoline 0.0000025 mM
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1,633,829 - +1.4
1,634,153 + nohA b1548 0.55 +0.9
1,634,153 + nohA b1548 0.55 -0.9
1,634,884 + ynfO b4533 0.44 -0.6
1,634,884 + ynfO b4533 0.44 -0.1
1,634,916 + ynfO b4533 0.58 +2.0
1,634,916 + ynfO b4533 0.58 +1.1
1,635,711 - b1550 b1550 0.45 -0.3
1,635,711 - b1550 b1550 0.45 +0.4
1,635,823 - -0.6
1,635,823 - +0.2
1,635,847 - -0.7
1,635,847 - -0.5

Or see this region's nucleotide sequence