Strain Fitness in Escherichia coli BW25113 around b3169

Experiment: LB Anaerobic with 8-hydroxy-nitroquinoline 0.0000025 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntinfB and nusA are separated by 24 nucleotidesnusA and rimP are separated by 27 nucleotidesrimP and metY are separated by 206 nucleotides b3168: infB - translation initiation factor IF-2 (NCBI), at 3,311,364 to 3,314,036 infB b3169: nusA - transcription elongation factor NusA (NCBI), at 3,314,061 to 3,315,548 nusA b3170: rimP - conserved protein (RefSeq), at 3,315,576 to 3,316,028 rimP b3171: metY - tRNA-Met (NCBI), at 3,316,235 to 3,316,311 metY Position (kb) 3314 3315 3316Strain fitness (log2 ratio) -2 -1 0 1at 3313.766 kb on - strand, within infBat 3313.842 kb on - strandat 3313.932 kb on - strandat 3313.976 kb on - strandat 3314.038 kb on - strandat 3314.051 kb on - strandat 3314.051 kb on - strandat 3314.233 kb on - strand, within nusAat 3314.249 kb on - strand, within nusAat 3314.252 kb on - strand, within nusAat 3314.302 kb on - strand, within nusAat 3314.302 kb on - strand, within nusAat 3314.302 kb on - strand, within nusAat 3316.026 kb on - strandat 3316.402 kb on + strandat 3316.506 kb on - strandat 3316.506 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Anaerobic with 8-hydroxy-nitroquinoline 0.0000025 mM
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3,313,766 - infB b3168 0.90 +0.0
3,313,842 - -0.2
3,313,932 - -0.8
3,313,976 - +1.1
3,314,038 - -0.4
3,314,051 - +0.3
3,314,051 - -0.1
3,314,233 - nusA b3169 0.12 +1.0
3,314,249 - nusA b3169 0.13 +0.4
3,314,252 - nusA b3169 0.13 +0.8
3,314,302 - nusA b3169 0.16 +0.5
3,314,302 - nusA b3169 0.16 -0.4
3,314,302 - nusA b3169 0.16 +1.1
3,316,026 - +0.2
3,316,402 + -1.2
3,316,506 - -2.4
3,316,506 - +0.5

Or see this region's nucleotide sequence