Strain Fitness in Escherichia coli BW25113 around b2726

Experiment: LB Anaerobic with 8-hydroxy-nitroquinoline 0.0000025 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nthycB and hycA are separated by 124 nucleotideshycA and hypA are separated by 211 nucleotideshypA and hypB are separated by 3 nucleotideshypB and hypC overlap by 10 nucleotides b2724: hycB - hydrogenase 3, Fe-S subunit (NCBI), at 2,847,260 to 2,847,871 hycB b2725: hycA - regulator of the transcriptional regulator FhlA (NCBI), at 2,847,996 to 2,848,457 hycA b2726: hypA - protein involved in nickel insertion into hydrogenases 3 (NCBI), at 2,848,669 to 2,849,019 hypA b2727: hypB - GTP hydrolase involved in nickel liganding into hydrogenases (NCBI), at 2,849,023 to 2,849,895 hypB b2728: hypC - protein required for maturation of hydrogenases 1 and 3 (NCBI), at 2,849,886 to 2,850,158 hypC Position (kb) 2848 2849 2850Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 2847.703 kb on + strand, within hycBat 2847.711 kb on - strand, within hycBat 2847.722 kb on - strand, within hycBat 2847.761 kb on - strand, within hycBat 2847.822 kb on - strandat 2847.829 kb on + strandat 2847.829 kb on + strandat 2847.888 kb on - strandat 2847.953 kb on - strandat 2848.071 kb on - strand, within hycAat 2848.073 kb on - strand, within hycAat 2848.073 kb on - strand, within hycAat 2848.082 kb on - strand, within hycAat 2848.153 kb on - strand, within hycAat 2848.153 kb on - strand, within hycAat 2848.157 kb on + strand, within hycAat 2848.157 kb on + strand, within hycAat 2848.253 kb on - strand, within hycAat 2848.253 kb on - strand, within hycAat 2848.684 kb on - strandat 2848.684 kb on - strandat 2848.684 kb on - strandat 2848.719 kb on - strand, within hypAat 2848.850 kb on - strand, within hypAat 2848.892 kb on + strand, within hypAat 2848.912 kb on + strand, within hypAat 2848.944 kb on + strand, within hypAat 2848.965 kb on - strand, within hypAat 2848.965 kb on - strand, within hypAat 2849.025 kb on + strandat 2849.025 kb on + strandat 2849.290 kb on - strand, within hypBat 2849.290 kb on - strand, within hypBat 2849.345 kb on + strand, within hypBat 2849.390 kb on - strand, within hypBat 2849.430 kb on + strand, within hypBat 2849.430 kb on + strand, within hypBat 2849.486 kb on + strand, within hypBat 2849.486 kb on + strand, within hypBat 2849.494 kb on + strand, within hypBat 2849.507 kb on - strand, within hypBat 2849.581 kb on - strand, within hypBat 2849.748 kb on + strand, within hypBat 2849.884 kb on - strandat 2849.917 kb on + strand, within hypCat 2849.917 kb on + strand, within hypCat 2849.917 kb on - strand, within hypCat 2849.917 kb on - strand, within hypC

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Anaerobic with 8-hydroxy-nitroquinoline 0.0000025 mM
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2,847,703 + hycB b2724 0.72 +1.4
2,847,711 - hycB b2724 0.74 -2.0
2,847,722 - hycB b2724 0.75 -0.2
2,847,761 - hycB b2724 0.82 +0.2
2,847,822 - +0.5
2,847,829 + +1.8
2,847,829 + -0.2
2,847,888 - -0.4
2,847,953 - +0.0
2,848,071 - hycA b2725 0.16 -2.0
2,848,073 - hycA b2725 0.17 -0.2
2,848,073 - hycA b2725 0.17 -0.9
2,848,082 - hycA b2725 0.19 -0.0
2,848,153 - hycA b2725 0.34 +0.7
2,848,153 - hycA b2725 0.34 -0.0
2,848,157 + hycA b2725 0.35 +2.3
2,848,157 + hycA b2725 0.35 -0.3
2,848,253 - hycA b2725 0.56 -0.2
2,848,253 - hycA b2725 0.56 +0.0
2,848,684 - +0.1
2,848,684 - -0.2
2,848,684 - +0.7
2,848,719 - hypA b2726 0.14 -0.3
2,848,850 - hypA b2726 0.52 -2.8
2,848,892 + hypA b2726 0.64 +0.4
2,848,912 + hypA b2726 0.69 +0.1
2,848,944 + hypA b2726 0.78 +0.4
2,848,965 - hypA b2726 0.84 +1.1
2,848,965 - hypA b2726 0.84 +1.0
2,849,025 + -1.4
2,849,025 + +0.6
2,849,290 - hypB b2727 0.31 -0.2
2,849,290 - hypB b2727 0.31 -0.0
2,849,345 + hypB b2727 0.37 -0.1
2,849,390 - hypB b2727 0.42 -0.3
2,849,430 + hypB b2727 0.47 +0.1
2,849,430 + hypB b2727 0.47 -1.5
2,849,486 + hypB b2727 0.53 -0.5
2,849,486 + hypB b2727 0.53 -1.8
2,849,494 + hypB b2727 0.54 +0.5
2,849,507 - hypB b2727 0.55 +0.5
2,849,581 - hypB b2727 0.64 +0.5
2,849,748 + hypB b2727 0.83 +0.1
2,849,884 - -0.5
2,849,917 + hypC b2728 0.11 -0.6
2,849,917 + hypC b2728 0.11 -0.1
2,849,917 - hypC b2728 0.11 -0.1
2,849,917 - hypC b2728 0.11 +0.2

Or see this region's nucleotide sequence