Strain Fitness in Escherichia coli BW25113 around b2674

Experiment: LB Anaerobic with 8-hydroxy-nitroquinoline 0.0000025 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntygaC and ygaM are separated by 139 nucleotidesygaM and nrdH are separated by 247 nucleotidesnrdH and nrdI overlap by 4 nucleotidesnrdI and nrdE overlap by 28 nucleotides b2671: ygaC - hypothetical protein (NCBI), at 2,797,672 to 2,798,016 ygaC b2672: ygaM - orf, hypothetical protein (VIMSS), at 2,798,156 to 2,798,497 ygaM b2673: nrdH - glutaredoxin-like protein (NCBI), at 2,798,745 to 2,798,990 nrdH b2674: nrdI - hypothetical protein (NCBI), at 2,798,987 to 2,799,397 nrdI b2675: nrdE - ribonucleotide-diphosphate reductase alpha subunit (NCBI), at 2,799,370 to 2,801,514 nrdE Position (kb) 2798 2799 2800Strain fitness (log2 ratio) -1 0 1 2at 2798.044 kb on + strandat 2798.044 kb on + strandat 2798.048 kb on + strandat 2798.049 kb on + strandat 2798.049 kb on + strandat 2798.216 kb on + strand, within ygaMat 2798.340 kb on + strand, within ygaMat 2798.387 kb on + strand, within ygaMat 2798.395 kb on - strand, within ygaMat 2798.427 kb on + strand, within ygaMat 2798.481 kb on - strandat 2798.481 kb on - strandat 2798.600 kb on + strandat 2798.614 kb on - strandat 2798.834 kb on + strand, within nrdHat 2798.849 kb on + strand, within nrdHat 2798.917 kb on + strand, within nrdHat 2798.917 kb on + strand, within nrdHat 2798.983 kb on + strandat 2798.983 kb on + strandat 2798.991 kb on - strandat 2799.158 kb on + strand, within nrdIat 2799.191 kb on + strand, within nrdIat 2799.251 kb on + strand, within nrdIat 2799.410 kb on - strandat 2799.505 kb on - strandat 2799.517 kb on + strandat 2799.535 kb on - strandat 2799.535 kb on - strandat 2799.535 kb on - strandat 2799.535 kb on - strandat 2799.631 kb on + strand, within nrdEat 2799.631 kb on - strand, within nrdEat 2799.756 kb on + strand, within nrdEat 2799.764 kb on - strand, within nrdEat 2799.764 kb on - strand, within nrdEat 2799.784 kb on - strand, within nrdEat 2799.784 kb on - strand, within nrdEat 2800.103 kb on - strand, within nrdEat 2800.115 kb on + strand, within nrdEat 2800.151 kb on + strand, within nrdEat 2800.168 kb on - strand, within nrdEat 2800.168 kb on - strand, within nrdEat 2800.248 kb on + strand, within nrdE

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Anaerobic with 8-hydroxy-nitroquinoline 0.0000025 mM
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2,798,044 + +1.3
2,798,044 + +0.2
2,798,048 + +0.2
2,798,049 + +0.7
2,798,049 + +0.7
2,798,216 + ygaM b2672 0.18 +0.2
2,798,340 + ygaM b2672 0.54 -0.5
2,798,387 + ygaM b2672 0.68 -0.4
2,798,395 - ygaM b2672 0.70 +0.3
2,798,427 + ygaM b2672 0.79 +0.8
2,798,481 - +0.7
2,798,481 - +0.7
2,798,600 + -0.1
2,798,614 - -0.1
2,798,834 + nrdH b2673 0.36 -0.9
2,798,849 + nrdH b2673 0.42 +0.4
2,798,917 + nrdH b2673 0.70 -0.5
2,798,917 + nrdH b2673 0.70 +0.3
2,798,983 + +1.7
2,798,983 + +0.4
2,798,991 - +0.2
2,799,158 + nrdI b2674 0.42 +0.8
2,799,191 + nrdI b2674 0.50 +0.1
2,799,251 + nrdI b2674 0.64 +0.5
2,799,410 - -0.9
2,799,505 - +0.4
2,799,517 + -0.6
2,799,535 - -0.7
2,799,535 - -0.3
2,799,535 - +0.1
2,799,535 - -0.3
2,799,631 + nrdE b2675 0.12 +0.1
2,799,631 - nrdE b2675 0.12 +0.7
2,799,756 + nrdE b2675 0.18 +0.8
2,799,764 - nrdE b2675 0.18 -0.1
2,799,764 - nrdE b2675 0.18 -0.0
2,799,784 - nrdE b2675 0.19 -0.2
2,799,784 - nrdE b2675 0.19 -0.4
2,800,103 - nrdE b2675 0.34 +0.9
2,800,115 + nrdE b2675 0.35 +1.9
2,800,151 + nrdE b2675 0.36 -0.6
2,800,168 - nrdE b2675 0.37 +1.1
2,800,168 - nrdE b2675 0.37 +0.7
2,800,248 + nrdE b2675 0.41 +0.1

Or see this region's nucleotide sequence