Strain Fitness in Escherichia coli BW25113 around b2211

Experiment: LB Anaerobic with 8-hydroxy-nitroquinoline 0.0000025 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntmqo and yojI are separated by 217 nucleotidesyojI and alkB are separated by 75 nucleotidesalkB and ada overlap by 1 nucleotides b2210: mqo - malate:quinone oxidoreductase (NCBI), at 2,303,130 to 2,304,776 mqo b2211: yojI - fused predicted multidrug transport subunits of ABC superfamily: membrane component/ATP-binding component (NCBI), at 2,304,994 to 2,306,637 yojI b2212: alkB - oxidative demethylase of N1-methyladenine or N3-methylcytosine DNA lesions (NCBI), at 2,306,713 to 2,307,363 alkB b2213: ada - fused DNA-binding transcriptional dual regulator/O6-methylguanine-DNA methyltransferase (NCBI), at 2,307,363 to 2,308,427 ada Position (kb) 2304 2305 2306 2307Strain fitness (log2 ratio) -1 0 1at 2304.051 kb on - strand, within mqoat 2304.051 kb on - strand, within mqoat 2304.174 kb on - strand, within mqoat 2304.174 kb on - strand, within mqoat 2304.237 kb on + strandat 2304.388 kb on + strand, within mqoat 2304.409 kb on + strand, within mqoat 2304.417 kb on - strand, within mqoat 2304.444 kb on - strand, within mqoat 2304.524 kb on + strand, within mqoat 2304.577 kb on + strand, within mqoat 2304.578 kb on + strand, within mqoat 2304.578 kb on + strand, within mqoat 2304.991 kb on + strandat 2305.038 kb on + strandat 2305.038 kb on + strandat 2305.045 kb on + strandat 2305.067 kb on - strandat 2305.102 kb on + strandat 2305.120 kb on - strandat 2305.289 kb on + strand, within yojIat 2305.302 kb on + strand, within yojIat 2305.390 kb on - strand, within yojIat 2305.439 kb on - strand, within yojIat 2305.529 kb on + strand, within yojIat 2305.616 kb on - strand, within yojIat 2305.720 kb on - strand, within yojIat 2305.720 kb on - strand, within yojIat 2305.747 kb on + strand, within yojIat 2305.747 kb on + strand, within yojIat 2305.760 kb on + strand, within yojIat 2305.763 kb on + strand, within yojIat 2305.799 kb on - strand, within yojIat 2305.829 kb on + strand, within yojIat 2305.848 kb on + strand, within yojIat 2305.890 kb on - strand, within yojIat 2305.935 kb on - strand, within yojIat 2306.040 kb on - strand, within yojIat 2306.078 kb on + strand, within yojIat 2306.139 kb on + strand, within yojIat 2306.139 kb on + strand, within yojIat 2306.337 kb on + strand, within yojIat 2306.337 kb on + strand, within yojIat 2306.433 kb on - strand, within yojIat 2306.444 kb on - strand, within yojIat 2306.444 kb on - strand, within yojIat 2306.444 kb on - strand, within yojIat 2306.500 kb on + strandat 2306.572 kb on + strandat 2306.572 kb on + strandat 2306.783 kb on + strand, within alkBat 2306.801 kb on - strand, within alkBat 2306.942 kb on - strand, within alkBat 2306.942 kb on - strand, within alkBat 2307.059 kb on + strand, within alkBat 2307.168 kb on - strand, within alkBat 2307.181 kb on - strand, within alkBat 2307.336 kb on + strandat 2307.336 kb on + strandat 2307.336 kb on + strandat 2307.368 kb on - strandat 2307.393 kb on + strandat 2307.549 kb on - strand, within adaat 2307.562 kb on - strand, within adaat 2307.583 kb on + strand, within adaat 2307.593 kb on + strand, within ada

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Anaerobic with 8-hydroxy-nitroquinoline 0.0000025 mM
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2,304,051 - mqo b2210 0.56 +0.5
2,304,051 - mqo b2210 0.56 -1.4
2,304,174 - mqo b2210 0.63 -0.0
2,304,174 - mqo b2210 0.63 +0.1
2,304,237 + -0.1
2,304,388 + mqo b2210 0.76 -0.1
2,304,409 + mqo b2210 0.78 +0.4
2,304,417 - mqo b2210 0.78 -1.7
2,304,444 - mqo b2210 0.80 +0.5
2,304,524 + mqo b2210 0.85 +0.2
2,304,577 + mqo b2210 0.88 -0.6
2,304,578 + mqo b2210 0.88 -0.5
2,304,578 + mqo b2210 0.88 +1.5
2,304,991 + +0.1
2,305,038 + -1.5
2,305,038 + +0.5
2,305,045 + +0.3
2,305,067 - -0.6
2,305,102 + -0.1
2,305,120 - +0.6
2,305,289 + yojI b2211 0.18 -0.0
2,305,302 + yojI b2211 0.19 +0.5
2,305,390 - yojI b2211 0.24 +0.7
2,305,439 - yojI b2211 0.27 +0.5
2,305,529 + yojI b2211 0.33 +0.1
2,305,616 - yojI b2211 0.38 +0.3
2,305,720 - yojI b2211 0.44 -0.0
2,305,720 - yojI b2211 0.44 +0.3
2,305,747 + yojI b2211 0.46 +0.2
2,305,747 + yojI b2211 0.46 -0.5
2,305,760 + yojI b2211 0.47 +0.9
2,305,763 + yojI b2211 0.47 +0.4
2,305,799 - yojI b2211 0.49 +0.3
2,305,829 + yojI b2211 0.51 +0.4
2,305,848 + yojI b2211 0.52 -1.1
2,305,890 - yojI b2211 0.55 -0.3
2,305,935 - yojI b2211 0.57 +0.7
2,306,040 - yojI b2211 0.64 +0.7
2,306,078 + yojI b2211 0.66 +0.1
2,306,139 + yojI b2211 0.70 +0.5
2,306,139 + yojI b2211 0.70 -0.1
2,306,337 + yojI b2211 0.82 +1.5
2,306,337 + yojI b2211 0.82 +0.3
2,306,433 - yojI b2211 0.88 +1.1
2,306,444 - yojI b2211 0.88 +0.5
2,306,444 - yojI b2211 0.88 +0.8
2,306,444 - yojI b2211 0.88 +0.7
2,306,500 + +0.8
2,306,572 + -0.3
2,306,572 + +0.3
2,306,783 + alkB b2212 0.11 +0.5
2,306,801 - alkB b2212 0.14 -0.8
2,306,942 - alkB b2212 0.35 +1.6
2,306,942 - alkB b2212 0.35 +0.3
2,307,059 + alkB b2212 0.53 -0.4
2,307,168 - alkB b2212 0.70 -1.0
2,307,181 - alkB b2212 0.72 +0.9
2,307,336 + +1.3
2,307,336 + +0.2
2,307,336 + +0.1
2,307,368 - -0.1
2,307,393 + -0.7
2,307,549 - ada b2213 0.17 -1.3
2,307,562 - ada b2213 0.19 +1.5
2,307,583 + ada b2213 0.21 -0.1
2,307,593 + ada b2213 0.22 +0.5

Or see this region's nucleotide sequence