Strain Fitness in Escherichia coli BW25113 around b2060

Experiment: LB Anaerobic with 8-hydroxy-nitroquinoline 0.0000025 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntwcaB and wcaA are separated by 2 nucleotideswcaA and b2060 are separated by 92 nucleotidesb2060 and wzb are separated by 2 nucleotideswzb and wza are separated by 5 nucleotides b2058: wcaB - predicted acyl transferase (NCBI), at 2,130,091 to 2,130,579 wcaB b2059: wcaA - predicted glycosyl transferase (NCBI), at 2,130,582 to 2,131,421 wcaA b2060: b2060 - orf, hypothetical protein (VIMSS), at 2,131,514 to 2,133,676 b2060 b2061: wzb - protein-tyrosine phosphatase (NCBI), at 2,133,679 to 2,134,122 wzb b2062: wza - lipoprotein required for capsular polysaccharide translocation through the outer membrane (NCBI), at 2,134,128 to 2,135,267 wza Position (kb) 2131 2132 2133 2134Strain fitness (log2 ratio) -1 0 1 2at 2130.625 kb on + strandat 2130.691 kb on - strand, within wcaAat 2130.691 kb on - strand, within wcaAat 2130.692 kb on - strand, within wcaAat 2130.813 kb on + strand, within wcaAat 2130.841 kb on + strand, within wcaAat 2130.889 kb on - strand, within wcaAat 2131.196 kb on - strand, within wcaAat 2131.212 kb on + strand, within wcaAat 2131.306 kb on - strand, within wcaAat 2131.332 kb on - strand, within wcaAat 2131.395 kb on + strandat 2131.395 kb on + strandat 2131.421 kb on - strandat 2131.519 kb on - strandat 2131.589 kb on + strandat 2131.691 kb on - strandat 2131.691 kb on - strandat 2131.830 kb on - strand, within b2060at 2131.840 kb on + strand, within b2060at 2131.840 kb on + strand, within b2060at 2131.903 kb on - strand, within b2060at 2131.960 kb on - strand, within b2060at 2132.123 kb on + strand, within b2060at 2132.297 kb on + strand, within b2060at 2132.405 kb on + strand, within b2060at 2132.469 kb on + strand, within b2060at 2132.535 kb on - strand, within b2060at 2132.698 kb on + strand, within b2060at 2132.698 kb on + strand, within b2060at 2132.842 kb on - strand, within b2060at 2132.973 kb on + strand, within b2060at 2132.981 kb on - strand, within b2060at 2133.037 kb on - strand, within b2060at 2133.348 kb on - strand, within b2060at 2133.494 kb on - strandat 2133.538 kb on - strandat 2133.685 kb on - strandat 2134.043 kb on + strand, within wzbat 2134.142 kb on - strandat 2134.142 kb on - strandat 2134.157 kb on - strandat 2134.208 kb on - strandat 2134.244 kb on - strand, within wzaat 2134.244 kb on - strand, within wzaat 2134.329 kb on + strand, within wzaat 2134.385 kb on + strand, within wzaat 2134.409 kb on + strand, within wzaat 2134.417 kb on - strand, within wzaat 2134.417 kb on - strand, within wzaat 2134.427 kb on - strand, within wzaat 2134.427 kb on - strand, within wzaat 2134.545 kb on + strand, within wzaat 2134.545 kb on + strand, within wzaat 2134.545 kb on + strand, within wzaat 2134.599 kb on + strand, within wzaat 2134.604 kb on + strand, within wza

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Anaerobic with 8-hydroxy-nitroquinoline 0.0000025 mM
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2,130,625 + +0.3
2,130,691 - wcaA b2059 0.13 -1.7
2,130,691 - wcaA b2059 0.13 +1.0
2,130,692 - wcaA b2059 0.13 +0.3
2,130,813 + wcaA b2059 0.28 -0.9
2,130,841 + wcaA b2059 0.31 +0.5
2,130,889 - wcaA b2059 0.37 +0.9
2,131,196 - wcaA b2059 0.73 +0.5
2,131,212 + wcaA b2059 0.75 -1.7
2,131,306 - wcaA b2059 0.86 +0.8
2,131,332 - wcaA b2059 0.89 +1.0
2,131,395 + -1.1
2,131,395 + +0.6
2,131,421 - -0.0
2,131,519 - +0.0
2,131,589 + +1.3
2,131,691 - -0.9
2,131,691 - -1.4
2,131,830 - b2060 b2060 0.15 -0.1
2,131,840 + b2060 b2060 0.15 +0.4
2,131,840 + b2060 b2060 0.15 -0.3
2,131,903 - b2060 b2060 0.18 +1.0
2,131,960 - b2060 b2060 0.21 +0.5
2,132,123 + b2060 b2060 0.28 +0.6
2,132,297 + b2060 b2060 0.36 +0.4
2,132,405 + b2060 b2060 0.41 -0.0
2,132,469 + b2060 b2060 0.44 -0.0
2,132,535 - b2060 b2060 0.47 +0.5
2,132,698 + b2060 b2060 0.55 +1.7
2,132,698 + b2060 b2060 0.55 +0.9
2,132,842 - b2060 b2060 0.61 +0.5
2,132,973 + b2060 b2060 0.67 +2.2
2,132,981 - b2060 b2060 0.68 +1.7
2,133,037 - b2060 b2060 0.70 +1.6
2,133,348 - b2060 b2060 0.85 +0.2
2,133,494 - -0.2
2,133,538 - +1.3
2,133,685 - +1.5
2,134,043 + wzb b2061 0.82 -0.3
2,134,142 - -0.1
2,134,142 - +0.2
2,134,157 - +0.3
2,134,208 - -1.0
2,134,244 - wza b2062 0.10 +1.6
2,134,244 - wza b2062 0.10 -0.3
2,134,329 + wza b2062 0.18 -0.8
2,134,385 + wza b2062 0.23 +0.1
2,134,409 + wza b2062 0.25 +0.8
2,134,417 - wza b2062 0.25 -0.3
2,134,417 - wza b2062 0.25 -0.5
2,134,427 - wza b2062 0.26 -0.2
2,134,427 - wza b2062 0.26 +0.6
2,134,545 + wza b2062 0.37 +0.5
2,134,545 + wza b2062 0.37 -0.5
2,134,545 + wza b2062 0.37 +0.2
2,134,599 + wza b2062 0.41 -0.6
2,134,604 + wza b2062 0.42 +0.1

Or see this region's nucleotide sequence