Strain Fitness in Escherichia coli BW25113 around b2059

Experiment: LB Anaerobic with 8-hydroxy-nitroquinoline 0.0000025 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntwcaC and wcaB overlap by 4 nucleotideswcaB and wcaA are separated by 2 nucleotideswcaA and b2060 are separated by 92 nucleotides b2057: wcaC - predicted glycosyl transferase (NCBI), at 2,128,877 to 2,130,094 wcaC b2058: wcaB - predicted acyl transferase (NCBI), at 2,130,091 to 2,130,579 wcaB b2059: wcaA - predicted glycosyl transferase (NCBI), at 2,130,582 to 2,131,421 wcaA b2060: b2060 - orf, hypothetical protein (VIMSS), at 2,131,514 to 2,133,676 b2060 Position (kb) 2130 2131 2132Strain fitness (log2 ratio) -2 -1 0 1at 2129.586 kb on + strand, within wcaCat 2129.690 kb on + strand, within wcaCat 2129.798 kb on + strand, within wcaCat 2129.803 kb on + strand, within wcaCat 2130.018 kb on - strandat 2130.025 kb on + strandat 2130.025 kb on + strandat 2130.046 kb on - strandat 2130.159 kb on + strand, within wcaBat 2130.625 kb on + strandat 2130.691 kb on - strand, within wcaAat 2130.691 kb on - strand, within wcaAat 2130.692 kb on - strand, within wcaAat 2130.813 kb on + strand, within wcaAat 2130.841 kb on + strand, within wcaAat 2130.889 kb on - strand, within wcaAat 2131.196 kb on - strand, within wcaAat 2131.212 kb on + strand, within wcaAat 2131.306 kb on - strand, within wcaAat 2131.332 kb on - strand, within wcaAat 2131.395 kb on + strandat 2131.395 kb on + strandat 2131.421 kb on - strandat 2131.519 kb on - strandat 2131.589 kb on + strandat 2131.691 kb on - strandat 2131.691 kb on - strandat 2131.830 kb on - strand, within b2060at 2131.840 kb on + strand, within b2060at 2131.840 kb on + strand, within b2060at 2131.903 kb on - strand, within b2060at 2131.960 kb on - strand, within b2060at 2132.123 kb on + strand, within b2060at 2132.297 kb on + strand, within b2060at 2132.405 kb on + strand, within b2060

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Anaerobic with 8-hydroxy-nitroquinoline 0.0000025 mM
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2,129,586 + wcaC b2057 0.58 -0.2
2,129,690 + wcaC b2057 0.67 +0.1
2,129,798 + wcaC b2057 0.76 +0.1
2,129,803 + wcaC b2057 0.76 +0.1
2,130,018 - -0.1
2,130,025 + -1.9
2,130,025 + +0.0
2,130,046 - -0.4
2,130,159 + wcaB b2058 0.14 +0.1
2,130,625 + +0.3
2,130,691 - wcaA b2059 0.13 -1.7
2,130,691 - wcaA b2059 0.13 +1.0
2,130,692 - wcaA b2059 0.13 +0.3
2,130,813 + wcaA b2059 0.28 -0.9
2,130,841 + wcaA b2059 0.31 +0.5
2,130,889 - wcaA b2059 0.37 +0.9
2,131,196 - wcaA b2059 0.73 +0.5
2,131,212 + wcaA b2059 0.75 -1.7
2,131,306 - wcaA b2059 0.86 +0.8
2,131,332 - wcaA b2059 0.89 +1.0
2,131,395 + -1.1
2,131,395 + +0.6
2,131,421 - -0.0
2,131,519 - +0.0
2,131,589 + +1.3
2,131,691 - -0.9
2,131,691 - -1.4
2,131,830 - b2060 b2060 0.15 -0.1
2,131,840 + b2060 b2060 0.15 +0.4
2,131,840 + b2060 b2060 0.15 -0.3
2,131,903 - b2060 b2060 0.18 +1.0
2,131,960 - b2060 b2060 0.21 +0.5
2,132,123 + b2060 b2060 0.28 +0.6
2,132,297 + b2060 b2060 0.36 +0.4
2,132,405 + b2060 b2060 0.41 -0.0

Or see this region's nucleotide sequence