Strain Fitness in Escherichia coli BW25113 around b2027

Experiment: LB Anaerobic with 8-hydroxy-nitroquinoline 0.0000025 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nthisF and hisI overlap by 7 nucleotideshisI and wzzB are separated by 95 nucleotideswzzB and ugd are separated by 145 nucleotides b2025: hisF - imidazole glycerol phosphate synthase subunit HisF (NCBI), at 2,093,868 to 2,094,644 hisF b2026: hisI - bifunctional phosphoribosyl-AMP cyclohydrolase/phosphoribosyl-ATP pyrophosphatase protein (NCBI), at 2,094,638 to 2,095,249 hisI b2027: wzzB - regulator of length of O-antigen component of lipopolysaccharide chains (VIMSS), at 2,095,345 to 2,096,325 wzzB b2028: ugd - UDP-glucose 6-dehydrogenase (NCBI), at 2,096,471 to 2,097,637 ugd Position (kb) 2095 2096 2097Strain fitness (log2 ratio) -2 -1 0 1 2at 2094.596 kb on + strandat 2094.596 kb on + strandat 2094.647 kb on + strandat 2094.712 kb on - strand, within hisIat 2094.712 kb on - strand, within hisIat 2094.716 kb on + strand, within hisIat 2094.869 kb on + strand, within hisIat 2094.907 kb on - strand, within hisIat 2094.907 kb on - strand, within hisIat 2095.032 kb on + strand, within hisIat 2095.033 kb on + strand, within hisIat 2095.033 kb on + strand, within hisIat 2095.124 kb on + strand, within hisIat 2095.167 kb on + strand, within hisIat 2095.202 kb on - strandat 2095.231 kb on + strandat 2095.386 kb on - strandat 2095.490 kb on + strand, within wzzBat 2095.494 kb on + strand, within wzzBat 2095.586 kb on - strand, within wzzBat 2095.586 kb on - strand, within wzzBat 2095.606 kb on - strand, within wzzBat 2095.719 kb on - strand, within wzzBat 2095.719 kb on - strand, within wzzBat 2095.729 kb on - strand, within wzzBat 2095.819 kb on + strand, within wzzBat 2095.819 kb on + strand, within wzzBat 2095.900 kb on + strand, within wzzBat 2095.900 kb on + strand, within wzzBat 2096.075 kb on + strand, within wzzBat 2096.075 kb on + strand, within wzzBat 2096.102 kb on + strand, within wzzBat 2096.163 kb on - strand, within wzzBat 2096.458 kb on + strandat 2096.458 kb on + strandat 2096.467 kb on + strandat 2096.480 kb on + strandat 2096.480 kb on + strandat 2096.486 kb on - strandat 2096.496 kb on + strandat 2096.498 kb on + strandat 2096.498 kb on + strandat 2096.519 kb on + strandat 2096.633 kb on + strand, within ugdat 2096.634 kb on + strand, within ugdat 2096.634 kb on + strand, within ugdat 2096.638 kb on - strand, within ugdat 2096.638 kb on - strand, within ugdat 2096.641 kb on - strand, within ugdat 2096.641 kb on - strand, within ugdat 2096.669 kb on - strand, within ugdat 2096.683 kb on + strand, within ugdat 2096.746 kb on + strand, within ugdat 2096.846 kb on - strand, within ugdat 2096.846 kb on - strand, within ugdat 2096.850 kb on + strand, within ugdat 2096.858 kb on - strand, within ugdat 2096.861 kb on - strand, within ugdat 2096.862 kb on - strand, within ugdat 2096.862 kb on - strand, within ugdat 2096.880 kb on + strand, within ugdat 2096.909 kb on + strand, within ugdat 2096.909 kb on + strand, within ugdat 2096.917 kb on - strand, within ugdat 2096.917 kb on - strand, within ugdat 2096.918 kb on - strand, within ugdat 2096.918 kb on - strand, within ugdat 2096.922 kb on + strand, within ugdat 2096.922 kb on + strand, within ugdat 2096.998 kb on - strand, within ugdat 2097.030 kb on - strand, within ugdat 2097.035 kb on - strand, within ugdat 2097.058 kb on - strand, within ugdat 2097.074 kb on + strand, within ugdat 2097.111 kb on + strand, within ugdat 2097.111 kb on + strand, within ugdat 2097.111 kb on + strand, within ugdat 2097.113 kb on + strand, within ugdat 2097.119 kb on - strand, within ugdat 2097.121 kb on + strand, within ugdat 2097.121 kb on - strand, within ugdat 2097.129 kb on - strand, within ugdat 2097.141 kb on + strand, within ugdat 2097.166 kb on - strand, within ugdat 2097.166 kb on - strand, within ugdat 2097.195 kb on + strand, within ugdat 2097.195 kb on + strand, within ugdat 2097.271 kb on + strand, within ugdat 2097.278 kb on + strand, within ugdat 2097.297 kb on + strand, within ugd

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Anaerobic with 8-hydroxy-nitroquinoline 0.0000025 mM
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2,094,596 + +0.9
2,094,596 + -1.0
2,094,647 + -0.8
2,094,712 - hisI b2026 0.12 -0.1
2,094,712 - hisI b2026 0.12 +0.5
2,094,716 + hisI b2026 0.13 +0.6
2,094,869 + hisI b2026 0.38 +0.1
2,094,907 - hisI b2026 0.44 -0.8
2,094,907 - hisI b2026 0.44 -0.1
2,095,032 + hisI b2026 0.64 +0.2
2,095,033 + hisI b2026 0.65 -0.5
2,095,033 + hisI b2026 0.65 -0.1
2,095,124 + hisI b2026 0.79 +0.1
2,095,167 + hisI b2026 0.86 -0.0
2,095,202 - -0.0
2,095,231 + +0.7
2,095,386 - +0.1
2,095,490 + wzzB b2027 0.15 -0.5
2,095,494 + wzzB b2027 0.15 +1.9
2,095,586 - wzzB b2027 0.25 +0.1
2,095,586 - wzzB b2027 0.25 +1.5
2,095,606 - wzzB b2027 0.27 -0.1
2,095,719 - wzzB b2027 0.38 +0.7
2,095,719 - wzzB b2027 0.38 +0.3
2,095,729 - wzzB b2027 0.39 +0.1
2,095,819 + wzzB b2027 0.48 -0.3
2,095,819 + wzzB b2027 0.48 +0.8
2,095,900 + wzzB b2027 0.57 +0.5
2,095,900 + wzzB b2027 0.57 +0.5
2,096,075 + wzzB b2027 0.74 -2.3
2,096,075 + wzzB b2027 0.74 +0.8
2,096,102 + wzzB b2027 0.77 +1.1
2,096,163 - wzzB b2027 0.83 +0.5
2,096,458 + -0.5
2,096,458 + +0.5
2,096,467 + +0.5
2,096,480 + +0.5
2,096,480 + -2.5
2,096,486 - -0.3
2,096,496 + -0.5
2,096,498 + +0.0
2,096,498 + -1.9
2,096,519 + +0.5
2,096,633 + ugd b2028 0.14 +1.5
2,096,634 + ugd b2028 0.14 +0.0
2,096,634 + ugd b2028 0.14 -0.1
2,096,638 - ugd b2028 0.14 -0.2
2,096,638 - ugd b2028 0.14 -0.3
2,096,641 - ugd b2028 0.15 +0.2
2,096,641 - ugd b2028 0.15 -0.0
2,096,669 - ugd b2028 0.17 +0.0
2,096,683 + ugd b2028 0.18 -2.1
2,096,746 + ugd b2028 0.24 +0.9
2,096,846 - ugd b2028 0.32 +0.0
2,096,846 - ugd b2028 0.32 -0.0
2,096,850 + ugd b2028 0.32 -0.7
2,096,858 - ugd b2028 0.33 -0.8
2,096,861 - ugd b2028 0.33 -0.8
2,096,862 - ugd b2028 0.34 +1.5
2,096,862 - ugd b2028 0.34 -0.5
2,096,880 + ugd b2028 0.35 -0.5
2,096,909 + ugd b2028 0.38 -0.6
2,096,909 + ugd b2028 0.38 -0.2
2,096,917 - ugd b2028 0.38 +0.5
2,096,917 - ugd b2028 0.38 -1.9
2,096,918 - ugd b2028 0.38 +1.5
2,096,918 - ugd b2028 0.38 -1.4
2,096,922 + ugd b2028 0.39 -0.2
2,096,922 + ugd b2028 0.39 -0.8
2,096,998 - ugd b2028 0.45 -0.5
2,097,030 - ugd b2028 0.48 +0.9
2,097,035 - ugd b2028 0.48 +0.4
2,097,058 - ugd b2028 0.50 -1.0
2,097,074 + ugd b2028 0.52 +1.7
2,097,111 + ugd b2028 0.55 +1.3
2,097,111 + ugd b2028 0.55 +1.9
2,097,111 + ugd b2028 0.55 -0.1
2,097,113 + ugd b2028 0.55 -0.1
2,097,119 - ugd b2028 0.56 +0.5
2,097,121 + ugd b2028 0.56 -0.3
2,097,121 - ugd b2028 0.56 -0.5
2,097,129 - ugd b2028 0.56 -1.5
2,097,141 + ugd b2028 0.57 +1.1
2,097,166 - ugd b2028 0.60 +0.5
2,097,166 - ugd b2028 0.60 +0.5
2,097,195 + ugd b2028 0.62 -0.3
2,097,195 + ugd b2028 0.62 +1.6
2,097,271 + ugd b2028 0.69 +0.8
2,097,278 + ugd b2028 0.69 -0.5
2,097,297 + ugd b2028 0.71 +0.0

Or see this region's nucleotide sequence