Strain Fitness in Escherichia coli BW25113 around b1562

Experiment: LB Anaerobic with 8-hydroxy-nitroquinoline 0.0000025 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntydfU and rem are separated by 346 nucleotidesrem and hokD are separated by 216 nucleotideshokD and relE are separated by 71 nucleotidesrelE and relB overlap by 1 nucleotidesrelB and ydfV are separated by 24 nucleotides b1560: ydfU - Qin prophage; predicted protein (RefSeq), at 1,641,279 to 1,642,328 ydfU b1561: rem - Qin prophage; predicted protein (NCBI), at 1,642,675 to 1,642,926 rem b1562: hokD - Qin prophage; small toxic polypeptide (NCBI), at 1,643,143 to 1,643,298 hokD b1563: relE - Qin prophage; toxin of the RelE-RelB toxin-antitoxin system (NCBI), at 1,643,370 to 1,643,657 relE b1564: relB - Qin prophage; bifunctional antitoxin of the RelE-RelB toxin-antitoxin system/ transcriptional repressor (NCBI), at 1,643,657 to 1,643,896 relB b1565: ydfV - Qin prophage; predicted protein (NCBI), at 1,643,921 to 1,644,226 ydfV Position (kb) 1643 1644Strain fitness (log2 ratio) -1 0 1 2 3at 1642.253 kb on - strandat 1642.270 kb on + strandat 1642.284 kb on - strandat 1642.299 kb on + strandat 1642.307 kb on - strandat 1642.382 kb on + strandat 1642.401 kb on + strandat 1642.406 kb on + strandat 1642.488 kb on + strandat 1642.531 kb on - strandat 1642.629 kb on + strandat 1642.629 kb on + strandat 1642.629 kb on + strandat 1642.645 kb on + strandat 1642.663 kb on - strandat 1642.769 kb on - strand, within remat 1643.141 kb on + strandat 1643.261 kb on - strand, within hokDat 1643.463 kb on - strand, within relEat 1643.463 kb on - strand, within relEat 1643.482 kb on + strand, within relEat 1643.661 kb on + strandat 1643.947 kb on - strandat 1643.963 kb on + strand, within ydfVat 1643.963 kb on + strand, within ydfVat 1643.963 kb on + strand, within ydfVat 1644.038 kb on - strand, within ydfVat 1644.041 kb on + strand, within ydfVat 1644.049 kb on - strand, within ydfVat 1644.187 kb on - strand, within ydfVat 1644.187 kb on - strand, within ydfVat 1644.248 kb on - strandat 1644.258 kb on - strandat 1644.283 kb on + strandat 1644.290 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Anaerobic with 8-hydroxy-nitroquinoline 0.0000025 mM
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1,642,253 - -0.6
1,642,270 + +0.3
1,642,284 - -0.7
1,642,299 + +0.9
1,642,307 - +0.3
1,642,382 + -0.4
1,642,401 + +0.5
1,642,406 + +0.4
1,642,488 + +0.1
1,642,531 - +0.4
1,642,629 + -0.6
1,642,629 + -1.2
1,642,629 + -0.6
1,642,645 + -0.6
1,642,663 - -1.0
1,642,769 - rem b1561 0.37 -0.1
1,643,141 + +0.3
1,643,261 - hokD b1562 0.76 +0.8
1,643,463 - relE b1563 0.32 -0.1
1,643,463 - relE b1563 0.32 +1.0
1,643,482 + relE b1563 0.39 +0.4
1,643,661 + +0.4
1,643,947 - +0.2
1,643,963 + ydfV b1565 0.14 -0.9
1,643,963 + ydfV b1565 0.14 -0.1
1,643,963 + ydfV b1565 0.14 -0.5
1,644,038 - ydfV b1565 0.38 -0.5
1,644,041 + ydfV b1565 0.39 -0.2
1,644,049 - ydfV b1565 0.42 +3.2
1,644,187 - ydfV b1565 0.87 -1.6
1,644,187 - ydfV b1565 0.87 -1.2
1,644,248 - +0.4
1,644,258 - +0.4
1,644,283 + +0.1
1,644,290 + -0.3

Or see this region's nucleotide sequence