Experiment: LB Anaerobic with 8-hydroxy-nitroquinoline 0.0000025 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ydfU and rem are separated by 346 nucleotides rem and hokD are separated by 216 nucleotides hokD and relE are separated by 71 nucleotides relE and relB overlap by 1 nucleotides relB and ydfV are separated by 24 nucleotides
b1560: ydfU - Qin prophage; predicted protein (RefSeq), at 1,641,279 to 1,642,328
ydfU
b1561: rem - Qin prophage; predicted protein (NCBI), at 1,642,675 to 1,642,926
rem
b1562: hokD - Qin prophage; small toxic polypeptide (NCBI), at 1,643,143 to 1,643,298
hokD
b1563: relE - Qin prophage; toxin of the RelE-RelB toxin-antitoxin system (NCBI), at 1,643,370 to 1,643,657
relE
b1564: relB - Qin prophage; bifunctional antitoxin of the RelE-RelB toxin-antitoxin system/ transcriptional repressor (NCBI), at 1,643,657 to 1,643,896
relB
b1565: ydfV - Qin prophage; predicted protein (NCBI), at 1,643,921 to 1,644,226
ydfV
Position (kb)
1643
1644 Strain fitness (log2 ratio)
-1
0
1
2
3 at 1642.253 kb on - strand at 1642.270 kb on + strand at 1642.284 kb on - strand at 1642.299 kb on + strand at 1642.307 kb on - strand at 1642.382 kb on + strand at 1642.401 kb on + strand at 1642.406 kb on + strand at 1642.488 kb on + strand at 1642.531 kb on - strand at 1642.629 kb on + strand at 1642.629 kb on + strand at 1642.629 kb on + strand at 1642.645 kb on + strand at 1642.663 kb on - strand at 1642.769 kb on - strand, within rem at 1643.141 kb on + strand at 1643.261 kb on - strand, within hokD at 1643.463 kb on - strand, within relE at 1643.463 kb on - strand, within relE at 1643.482 kb on + strand, within relE at 1643.661 kb on + strand at 1643.947 kb on - strand at 1643.963 kb on + strand, within ydfV at 1643.963 kb on + strand, within ydfV at 1643.963 kb on + strand, within ydfV at 1644.038 kb on - strand, within ydfV at 1644.041 kb on + strand, within ydfV at 1644.049 kb on - strand, within ydfV at 1644.187 kb on - strand, within ydfV at 1644.187 kb on - strand, within ydfV at 1644.248 kb on - strand at 1644.258 kb on - strand at 1644.283 kb on + strand at 1644.290 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with 8-hydroxy-nitroquinoline 0.0000025 mM remove 1,642,253 - -0.6 1,642,270 + +0.3 1,642,284 - -0.7 1,642,299 + +0.9 1,642,307 - +0.3 1,642,382 + -0.4 1,642,401 + +0.5 1,642,406 + +0.4 1,642,488 + +0.1 1,642,531 - +0.4 1,642,629 + -0.6 1,642,629 + -1.2 1,642,629 + -0.6 1,642,645 + -0.6 1,642,663 - -1.0 1,642,769 - rem b1561 0.37 -0.1 1,643,141 + +0.3 1,643,261 - hokD b1562 0.76 +0.8 1,643,463 - relE b1563 0.32 -0.1 1,643,463 - relE b1563 0.32 +1.0 1,643,482 + relE b1563 0.39 +0.4 1,643,661 + +0.4 1,643,947 - +0.2 1,643,963 + ydfV b1565 0.14 -0.9 1,643,963 + ydfV b1565 0.14 -0.1 1,643,963 + ydfV b1565 0.14 -0.5 1,644,038 - ydfV b1565 0.38 -0.5 1,644,041 + ydfV b1565 0.39 -0.2 1,644,049 - ydfV b1565 0.42 +3.2 1,644,187 - ydfV b1565 0.87 -1.6 1,644,187 - ydfV b1565 0.87 -1.2 1,644,248 - +0.4 1,644,258 - +0.4 1,644,283 + +0.1 1,644,290 + -0.3
Or see this region's nucleotide sequence