Strain Fitness in Escherichia coli BW25113 around b1480

Experiment: LB Anaerobic with 8-hydroxy-nitroquinoline 0.0000025 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntsfcA and sra are separated by 156 nucleotidessra and b1481 are separated by 101 nucleotidesb1481 and osmC are separated by 344 nucleotides b1479: sfcA - NAD-linked malate dehydrogenase (malic enzyme) (VIMSS), at 1,551,996 to 1,553,693 sfcA b1480: sra - 30S ribosomal subunit protein S22 (NCBI), at 1,553,850 to 1,553,987 sra b1481: b1481 - orf, hypothetical protein (VIMSS), at 1,554,089 to 1,554,304 b1481 b1482: osmC - osmotically inducible, stress-inducible membrane protein (NCBI), at 1,554,649 to 1,555,080 osmC Position (kb) 1553 1554Strain fitness (log2 ratio) -2 -1 0 1at 1553.155 kb on + strand, within sfcAat 1553.284 kb on - strand, within sfcAat 1553.427 kb on + strand, within sfcAat 1553.435 kb on - strand, within sfcAat 1553.588 kb on + strandat 1553.636 kb on + strandat 1553.636 kb on + strandat 1553.638 kb on + strandat 1553.647 kb on + strandat 1553.700 kb on - strandat 1553.833 kb on + strandat 1553.833 kb on + strandat 1553.951 kb on + strand, within sraat 1553.951 kb on + strand, within sraat 1553.951 kb on + strand, within sraat 1554.079 kb on + strandat 1554.082 kb on - strandat 1554.097 kb on - strandat 1554.101 kb on + strandat 1554.111 kb on - strand, within b1481at 1554.111 kb on - strand, within b1481at 1554.123 kb on - strand, within b1481at 1554.132 kb on + strand, within b1481at 1554.144 kb on - strand, within b1481at 1554.218 kb on + strand, within b1481at 1554.224 kb on - strand, within b1481at 1554.224 kb on - strand, within b1481at 1554.244 kb on - strand, within b1481at 1554.249 kb on + strand, within b1481at 1554.257 kb on - strand, within b1481at 1554.273 kb on + strand, within b1481at 1554.355 kb on - strandat 1554.365 kb on + strandat 1554.407 kb on + strandat 1554.407 kb on + strandat 1554.599 kb on + strandat 1554.599 kb on + strandat 1554.601 kb on + strandat 1554.639 kb on - strandat 1554.678 kb on + strandat 1554.678 kb on - strandat 1554.684 kb on - strandat 1554.697 kb on + strand, within osmCat 1554.707 kb on + strand, within osmCat 1554.718 kb on + strand, within osmCat 1554.723 kb on - strand, within osmC

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Anaerobic with 8-hydroxy-nitroquinoline 0.0000025 mM
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1,553,155 + sfcA b1479 0.68 -1.4
1,553,284 - sfcA b1479 0.76 -1.6
1,553,427 + sfcA b1479 0.84 +0.3
1,553,435 - sfcA b1479 0.85 -0.8
1,553,588 + -1.0
1,553,636 + +0.5
1,553,636 + +0.8
1,553,638 + +1.6
1,553,647 + -1.3
1,553,700 - -0.4
1,553,833 + +0.7
1,553,833 + +0.4
1,553,951 + sra b1480 0.73 +0.4
1,553,951 + sra b1480 0.73 +0.4
1,553,951 + sra b1480 0.73 +0.8
1,554,079 + -0.4
1,554,082 - -0.6
1,554,097 - +0.1
1,554,101 + +1.2
1,554,111 - b1481 b1481 0.10 +0.2
1,554,111 - b1481 b1481 0.10 +0.6
1,554,123 - b1481 b1481 0.16 +0.3
1,554,132 + b1481 b1481 0.20 -0.9
1,554,144 - b1481 b1481 0.25 -1.3
1,554,218 + b1481 b1481 0.60 -0.6
1,554,224 - b1481 b1481 0.62 +0.4
1,554,224 - b1481 b1481 0.62 +0.4
1,554,244 - b1481 b1481 0.72 +0.4
1,554,249 + b1481 b1481 0.74 -1.2
1,554,257 - b1481 b1481 0.78 +0.4
1,554,273 + b1481 b1481 0.85 +0.4
1,554,355 - +0.4
1,554,365 + -1.2
1,554,407 + +0.4
1,554,407 + +0.4
1,554,599 + -1.6
1,554,599 + +1.4
1,554,601 + -1.6
1,554,639 - -2.4
1,554,678 + +1.2
1,554,678 - +0.4
1,554,684 - +0.4
1,554,697 + osmC b1482 0.11 -0.2
1,554,707 + osmC b1482 0.13 -1.6
1,554,718 + osmC b1482 0.16 +0.4
1,554,723 - osmC b1482 0.17 +0.2

Or see this region's nucleotide sequence