Strain Fitness in Escherichia coli BW25113 around b1399

Experiment: LB Anaerobic with 8-hydroxy-nitroquinoline 0.0000025 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntpaaK and paaX are separated by 100 nucleotidespaaX and paaY overlap by 19 nucleotidespaaY and ynbG are separated by 103 nucleotidesynbG and ydbA are separated by 161 nucleotides b1398: paaK - phenylacetyl-CoA ligase (NCBI), at 1,460,149 to 1,461,462 paaK b1399: paaX - DNA-binding transcriptional repressor of phenylacetic acid degradation, aryl-CoA responsive (NCBI), at 1,461,563 to 1,462,513 paaX b1400: paaY - predicted hexapeptide repeat acetyltransferase (NCBI), at 1,462,495 to 1,463,085 paaY b4674: ynbG - expressed protein (RefSeq), at 1,463,189 to 1,463,254 ynbG b4492: ydbA - no description, at 1,463,416 to 1,465,928 ydbA Position (kb) 1461 1462 1463Strain fitness (log2 ratio) -2 -1 0 1 2at 1461.222 kb on - strand, within paaKat 1461.441 kb on + strandat 1461.562 kb on + strandat 1461.562 kb on + strandat 1461.638 kb on + strandat 1461.687 kb on + strand, within paaXat 1461.873 kb on + strand, within paaXat 1461.873 kb on + strand, within paaXat 1461.938 kb on + strand, within paaXat 1461.978 kb on + strand, within paaXat 1462.399 kb on - strand, within paaXat 1462.521 kb on + strandat 1462.521 kb on + strandat 1462.975 kb on + strand, within paaYat 1462.975 kb on + strand, within paaYat 1462.988 kb on + strand, within paaYat 1463.000 kb on + strand, within paaYat 1463.053 kb on + strandat 1463.053 kb on + strandat 1463.080 kb on + strandat 1463.082 kb on + strandat 1463.499 kb on - strandat 1463.499 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Anaerobic with 8-hydroxy-nitroquinoline 0.0000025 mM
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1,461,222 - paaK b1398 0.82 +0.1
1,461,441 + +0.2
1,461,562 + +0.8
1,461,562 + +0.2
1,461,638 + +0.1
1,461,687 + paaX b1399 0.13 +0.2
1,461,873 + paaX b1399 0.33 +0.5
1,461,873 + paaX b1399 0.33 +0.5
1,461,938 + paaX b1399 0.39 +2.5
1,461,978 + paaX b1399 0.44 +0.4
1,462,399 - paaX b1399 0.88 +0.2
1,462,521 + +1.0
1,462,521 + -0.7
1,462,975 + paaY b1400 0.81 -1.9
1,462,975 + paaY b1400 0.81 -0.6
1,462,988 + paaY b1400 0.83 -1.1
1,463,000 + paaY b1400 0.85 +1.3
1,463,053 + -0.1
1,463,053 + +0.0
1,463,080 + +0.0
1,463,082 + +0.9
1,463,499 - +0.4
1,463,499 - +0.4

Or see this region's nucleotide sequence