Strain Fitness in Escherichia coli BW25113 around b1251

Experiment: LB Anaerobic with 8-hydroxy-nitroquinoline 0.0000025 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntkch and yciI are separated by 299 nucleotidesyciI and tonB are separated by 223 nucleotidestonB and yciA are separated by 39 nucleotides b1250: kch - voltage-gated potassium channel (NCBI), at 1,307,040 to 1,308,293 kch b1251: yciI - orf, hypothetical protein (VIMSS), at 1,308,593 to 1,308,889 yciI b1252: tonB - membrane spanning protein in TonB-ExbB-ExbD complex (NCBI), at 1,309,113 to 1,309,832 tonB b1253: yciA - predicted hydrolase (NCBI), at 1,309,872 to 1,310,270 yciA Position (kb) 1308 1309Strain fitness (log2 ratio) -2 -1 0 1 2at 1307.613 kb on + strand, within kchat 1307.613 kb on + strand, within kchat 1307.620 kb on - strand, within kchat 1307.820 kb on - strand, within kchat 1307.827 kb on - strand, within kchat 1307.895 kb on - strand, within kchat 1307.994 kb on - strand, within kchat 1307.994 kb on - strand, within kchat 1308.003 kb on + strand, within kchat 1308.045 kb on + strand, within kchat 1308.061 kb on - strand, within kchat 1308.061 kb on - strand, within kchat 1308.068 kb on + strand, within kchat 1308.103 kb on + strand, within kchat 1308.110 kb on + strand, within kchat 1308.256 kb on - strandat 1308.285 kb on + strandat 1308.298 kb on - strandat 1308.300 kb on - strandat 1308.380 kb on + strandat 1308.656 kb on - strand, within yciIat 1308.663 kb on - strand, within yciIat 1308.679 kb on - strand, within yciIat 1308.679 kb on - strand, within yciIat 1308.732 kb on + strand, within yciIat 1308.741 kb on - strand, within yciIat 1308.744 kb on + strand, within yciIat 1308.744 kb on + strand, within yciIat 1309.193 kb on + strand, within tonBat 1309.201 kb on - strand, within tonBat 1309.201 kb on - strand, within tonBat 1309.201 kb on - strand, within tonBat 1309.474 kb on + strand, within tonBat 1309.533 kb on - strand, within tonBat 1309.533 kb on - strand, within tonBat 1309.610 kb on + strand, within tonBat 1309.705 kb on - strand, within tonBat 1309.705 kb on - strand, within tonB

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Anaerobic with 8-hydroxy-nitroquinoline 0.0000025 mM
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1,307,613 + kch b1250 0.46 -1.8
1,307,613 + kch b1250 0.46 -0.7
1,307,620 - kch b1250 0.46 +0.2
1,307,820 - kch b1250 0.62 +0.5
1,307,827 - kch b1250 0.63 -0.5
1,307,895 - kch b1250 0.68 +0.9
1,307,994 - kch b1250 0.76 +0.1
1,307,994 - kch b1250 0.76 +0.7
1,308,003 + kch b1250 0.77 +0.5
1,308,045 + kch b1250 0.80 +0.5
1,308,061 - kch b1250 0.81 +0.9
1,308,061 - kch b1250 0.81 -1.1
1,308,068 + kch b1250 0.82 -0.5
1,308,103 + kch b1250 0.85 -1.1
1,308,110 + kch b1250 0.85 +0.5
1,308,256 - +1.5
1,308,285 + -1.5
1,308,298 - +0.5
1,308,300 - -1.5
1,308,380 + -1.1
1,308,656 - yciI b1251 0.21 +1.0
1,308,663 - yciI b1251 0.24 -0.5
1,308,679 - yciI b1251 0.29 +0.9
1,308,679 - yciI b1251 0.29 +0.5
1,308,732 + yciI b1251 0.47 +0.5
1,308,741 - yciI b1251 0.50 +0.6
1,308,744 + yciI b1251 0.51 +1.5
1,308,744 + yciI b1251 0.51 +1.1
1,309,193 + tonB b1252 0.11 -0.8
1,309,201 - tonB b1252 0.12 +1.1
1,309,201 - tonB b1252 0.12 +0.1
1,309,201 - tonB b1252 0.12 -1.8
1,309,474 + tonB b1252 0.50 -0.5
1,309,533 - tonB b1252 0.58 +0.2
1,309,533 - tonB b1252 0.58 -0.9
1,309,610 + tonB b1252 0.69 +0.5
1,309,705 - tonB b1252 0.82 +2.1
1,309,705 - tonB b1252 0.82 -0.2

Or see this region's nucleotide sequence