Experiment: LB Anaerobic with 8-hydroxy-nitroquinoline 0.0000025 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ybaT and cueR are separated by 124 nucleotides cueR and ybbJ are separated by 0 nucleotides ybbJ and ybbK overlap by 4 nucleotides
b0486: ybaT - predicted transporter (NCBI), at 511,800 to 513,092
ybaT
b0487: cueR - DNA-binding transcriptional activator of copper-responsive regulon genes (NCBI), at 513,217 to 513,624
cueR
b0488: ybbJ - conserved inner membrane protein (RefSeq), at 513,625 to 514,083
ybbJ
b0489: ybbK - predicted protease, membrane anchored (NCBI), at 514,080 to 514,997
ybbK
Position (kb) 513
514 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 512.239 kb on - strand, within ybaT at 512.413 kb on - strand, within ybaT at 512.414 kb on - strand, within ybaT at 512.514 kb on - strand, within ybaT at 512.567 kb on - strand, within ybaT at 512.604 kb on + strand, within ybaT at 512.771 kb on + strand, within ybaT at 512.830 kb on + strand, within ybaT at 512.830 kb on + strand, within ybaT at 512.846 kb on - strand, within ybaT at 512.886 kb on + strand, within ybaT at 512.890 kb on + strand, within ybaT at 512.953 kb on + strand, within ybaT at 512.958 kb on + strand, within ybaT at 512.958 kb on + strand, within ybaT at 512.991 kb on + strand at 512.998 kb on + strand at 512.998 kb on + strand at 513.013 kb on - strand at 513.013 kb on - strand at 513.039 kb on + strand at 513.039 kb on + strand at 513.057 kb on - strand at 513.057 kb on - strand at 513.057 kb on - strand at 513.077 kb on + strand at 513.077 kb on + strand at 513.095 kb on - strand at 513.107 kb on + strand at 513.109 kb on + strand at 513.182 kb on + strand at 513.205 kb on - strand at 513.287 kb on - strand, within cueR at 513.295 kb on - strand, within cueR at 513.431 kb on - strand, within cueR at 513.480 kb on + strand, within cueR at 513.537 kb on - strand, within cueR at 513.537 kb on - strand, within cueR at 513.700 kb on - strand, within ybbJ at 513.728 kb on - strand, within ybbJ at 513.742 kb on + strand, within ybbJ at 513.742 kb on + strand, within ybbJ at 513.949 kb on - strand, within ybbJ at 513.949 kb on - strand, within ybbJ at 513.958 kb on + strand, within ybbJ at 513.958 kb on + strand, within ybbJ at 513.977 kb on + strand, within ybbJ at 514.116 kb on - strand at 514.116 kb on - strand at 514.118 kb on - strand at 514.118 kb on - strand at 514.129 kb on - strand at 514.133 kb on - strand at 514.133 kb on - strand at 514.142 kb on + strand at 514.142 kb on + strand at 514.185 kb on + strand, within ybbK at 514.473 kb on - strand, within ybbK at 514.546 kb on - strand, within ybbK
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with 8-hydroxy-nitroquinoline 0.0000025 mM remove 512,239 - ybaT b0486 0.34 +0.2 512,413 - ybaT b0486 0.47 +0.4 512,414 - ybaT b0486 0.47 -1.4 512,514 - ybaT b0486 0.55 +0.3 512,567 - ybaT b0486 0.59 -0.1 512,604 + ybaT b0486 0.62 -0.6 512,771 + ybaT b0486 0.75 +0.5 512,830 + ybaT b0486 0.80 +0.4 512,830 + ybaT b0486 0.80 +0.4 512,846 - ybaT b0486 0.81 -0.1 512,886 + ybaT b0486 0.84 -0.3 512,890 + ybaT b0486 0.84 -1.0 512,953 + ybaT b0486 0.89 -0.3 512,958 + ybaT b0486 0.90 -1.1 512,958 + ybaT b0486 0.90 -1.3 512,991 + +0.3 512,998 + +0.1 512,998 + +0.0 513,013 - +0.2 513,013 - +0.7 513,039 + -0.6 513,039 + +0.2 513,057 - +0.7 513,057 - -0.2 513,057 - +0.7 513,077 + -0.1 513,077 + +1.0 513,095 - -0.8 513,107 + +1.0 513,109 + +0.3 513,182 + +0.1 513,205 - +0.1 513,287 - cueR b0487 0.17 +1.0 513,295 - cueR b0487 0.19 +0.7 513,431 - cueR b0487 0.52 +0.2 513,480 + cueR b0487 0.64 -0.1 513,537 - cueR b0487 0.78 -1.1 513,537 - cueR b0487 0.78 -0.3 513,700 - ybbJ b0488 0.16 -0.3 513,728 - ybbJ b0488 0.22 +0.4 513,742 + ybbJ b0488 0.25 +0.3 513,742 + ybbJ b0488 0.25 +0.7 513,949 - ybbJ b0488 0.71 -1.1 513,949 - ybbJ b0488 0.71 +1.5 513,958 + ybbJ b0488 0.73 -0.7 513,958 + ybbJ b0488 0.73 -0.6 513,977 + ybbJ b0488 0.77 -1.8 514,116 - -0.7 514,116 - +1.1 514,118 - -0.7 514,118 - +1.2 514,129 - -0.0 514,133 - +0.5 514,133 - -0.6 514,142 + -1.0 514,142 + +3.1 514,185 + ybbK b0489 0.11 -0.5 514,473 - ybbK b0489 0.43 -1.4 514,546 - ybbK b0489 0.51 +1.3
Or see this region's nucleotide sequence