Experiment: LB Anaerobic with 8-hydroxy-nitroquinoline 0.0000025 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ykfM and yafU are separated by 9 nucleotides yafU and yafF are separated by 3 nucleotides yafF and yafV are separated by 40 nucleotides yafV and ivy are separated by 153 nucleotides ivy and yafH are separated by 42 nucleotides
b4586: ykfM - hypothetical protein, no homologs (RefSeq), at 238,257 to 238,736
ykfM
b0218: yafU - predicted inner membrane protein (RefSeq), at 238,746 to 239,102
yafU
b4503: yafF - no description, at 239,106 to 239,378
yafF
b0219: yafV - predicted C-N hydrolase family amidase, NAD(P)-binding (NCBI), at 239,419 to 240,189
yafV
b0220: ivy - inhibitor of vertebrate C-lysozyme (NCBI), at 240,343 to 240,816
ivy
b0221: yafH - putative acyl-CoA dehydrogenase (EC 1.3.99.-) (VIMSS), at 240,859 to 243,303
yafH
Position (kb) 239
240
241 Strain fitness (log2 ratio) -2
-1
0
1
2 at 238.734 kb on - strand at 238.901 kb on + strand, within yafU at 238.910 kb on - strand, within yafU at 238.915 kb on - strand, within yafU at 238.915 kb on - strand, within yafU at 238.915 kb on - strand, within yafU at 239.013 kb on + strand, within yafU at 239.013 kb on + strand, within yafU at 239.013 kb on + strand, within yafU at 239.036 kb on - strand, within yafU at 239.036 kb on - strand, within yafU at 239.163 kb on + strand, within yafF at 239.166 kb on + strand, within yafF at 239.392 kb on - strand at 239.412 kb on + strand at 239.414 kb on - strand at 239.417 kb on + strand at 239.426 kb on + strand at 239.436 kb on - strand at 239.436 kb on - strand at 239.850 kb on - strand, within yafV at 239.850 kb on - strand, within yafV at 239.907 kb on + strand, within yafV at 240.066 kb on + strand, within yafV at 240.072 kb on + strand, within yafV at 240.098 kb on - strand, within yafV at 240.145 kb on + strand at 240.148 kb on + strand at 240.175 kb on + strand at 240.197 kb on - strand at 240.197 kb on - strand at 240.199 kb on - strand at 240.199 kb on - strand at 240.224 kb on + strand at 240.261 kb on - strand at 240.325 kb on + strand at 240.325 kb on + strand at 240.333 kb on + strand at 240.350 kb on + strand at 240.350 kb on + strand at 240.358 kb on - strand at 240.406 kb on + strand, within ivy at 240.493 kb on + strand, within ivy at 240.521 kb on - strand, within ivy at 240.544 kb on + strand, within ivy at 240.544 kb on + strand, within ivy at 240.608 kb on + strand, within ivy at 240.717 kb on + strand, within ivy at 240.743 kb on - strand, within ivy at 240.743 kb on - strand, within ivy at 240.843 kb on - strand at 240.939 kb on + strand at 240.939 kb on + strand at 241.035 kb on + strand at 241.035 kb on + strand at 241.158 kb on + strand, within yafH at 241.164 kb on + strand, within yafH at 241.164 kb on + strand, within yafH at 241.168 kb on - strand, within yafH
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with 8-hydroxy-nitroquinoline 0.0000025 mM remove 238,734 - +0.7 238,901 + yafU b0218 0.43 +0.7 238,910 - yafU b0218 0.46 +0.7 238,915 - yafU b0218 0.47 +0.7 238,915 - yafU b0218 0.47 -0.3 238,915 - yafU b0218 0.47 -0.3 239,013 + yafU b0218 0.75 +0.7 239,013 + yafU b0218 0.75 +0.7 239,013 + yafU b0218 0.75 -0.3 239,036 - yafU b0218 0.81 -0.1 239,036 - yafU b0218 0.81 +1.0 239,163 + yafF b4503 0.21 -1.3 239,166 + yafF b4503 0.22 -1.3 239,392 - -0.5 239,412 + -0.3 239,414 - +0.3 239,417 + -0.4 239,426 + +0.6 239,436 - -0.9 239,436 - -0.6 239,850 - yafV b0219 0.56 +0.9 239,850 - yafV b0219 0.56 +1.9 239,907 + yafV b0219 0.63 +0.1 240,066 + yafV b0219 0.84 +0.0 240,072 + yafV b0219 0.85 +0.6 240,098 - yafV b0219 0.88 -0.3 240,145 + -0.1 240,148 + -0.6 240,175 + -1.9 240,197 - -0.5 240,197 - -1.3 240,199 - +1.1 240,199 - +0.7 240,224 + -0.3 240,261 - -0.0 240,325 + -1.9 240,325 + -0.0 240,333 + +1.0 240,350 + -0.0 240,350 + +0.2 240,358 - -0.1 240,406 + ivy b0220 0.13 +0.2 240,493 + ivy b0220 0.32 +0.7 240,521 - ivy b0220 0.38 +1.1 240,544 + ivy b0220 0.42 -0.3 240,544 + ivy b0220 0.42 +0.2 240,608 + ivy b0220 0.56 +0.5 240,717 + ivy b0220 0.79 +0.3 240,743 - ivy b0220 0.84 +0.1 240,743 - ivy b0220 0.84 +1.7 240,843 - +0.1 240,939 + +0.1 240,939 + -0.2 241,035 + +0.5 241,035 + +0.4 241,158 + yafH b0221 0.12 -0.4 241,164 + yafH b0221 0.12 -0.1 241,164 + yafH b0221 0.12 -1.1 241,168 - yafH b0221 0.13 +0.0
Or see this region's nucleotide sequence