Experiment: LB Anaerobic with Zinc Pyrithione 0.00000125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yebO and yobG are separated by 74 nucleotides yobG and yobH are separated by 158 nucleotides yobH and kdgR are separated by 143 nucleotides
b1825: yebO - hypothetical protein (NCBI), at 1,906,285 to 1,906,572
yebO
b1826: yobG - hypothetical protein (NCBI), at 1,906,647 to 1,906,790
yobG
b4536: yobH - hypothetical protein (NCBI), at 1,906,949 to 1,907,188
yobH
b1827: kdgR - predicted DNA-binding transcriptional regulator (NCBI), at 1,907,332 to 1,908,123
kdgR
Position (kb)
1906
1907
1908 Strain fitness (log2 ratio)
-1
0
1 at 1906.101 kb on - strand at 1906.214 kb on - strand at 1906.230 kb on - strand at 1906.245 kb on - strand at 1906.248 kb on - strand at 1906.250 kb on - strand at 1906.256 kb on - strand at 1906.256 kb on - strand at 1906.300 kb on - strand at 1906.300 kb on - strand at 1906.588 kb on - strand at 1907.006 kb on - strand, within yobH at 1907.020 kb on - strand, within yobH at 1907.067 kb on + strand, within yobH at 1907.067 kb on + strand, within yobH at 1907.101 kb on + strand, within yobH at 1907.150 kb on + strand, within yobH at 1907.150 kb on + strand, within yobH at 1907.213 kb on - strand at 1907.226 kb on - strand at 1907.333 kb on - strand at 1907.333 kb on - strand at 1907.352 kb on - strand at 1907.389 kb on - strand at 1907.462 kb on - strand at 1907.560 kb on + strand, within kdgR at 1907.609 kb on - strand, within kdgR at 1907.609 kb on - strand, within kdgR at 1907.698 kb on - strand, within kdgR at 1908.131 kb on - strand at 1908.133 kb on - strand at 1908.151 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Zinc Pyrithione 0.00000125 mM remove 1,906,101 - +0.4 1,906,214 - -0.6 1,906,230 - -0.6 1,906,245 - +1.4 1,906,248 - -0.2 1,906,250 - -1.0 1,906,256 - -1.2 1,906,256 - +0.6 1,906,300 - +0.3 1,906,300 - +1.7 1,906,588 - +0.1 1,907,006 - yobH b4536 0.24 -0.6 1,907,020 - yobH b4536 0.30 -0.6 1,907,067 + yobH b4536 0.49 -0.8 1,907,067 + yobH b4536 0.49 -0.5 1,907,101 + yobH b4536 0.63 -0.1 1,907,150 + yobH b4536 0.84 -0.9 1,907,150 + yobH b4536 0.84 -1.1 1,907,213 - +1.4 1,907,226 - -1.2 1,907,333 - -0.3 1,907,333 - -0.4 1,907,352 - -1.0 1,907,389 - +1.2 1,907,462 - +1.2 1,907,560 + kdgR b1827 0.29 +0.4 1,907,609 - kdgR b1827 0.35 +0.4 1,907,609 - kdgR b1827 0.35 -0.3 1,907,698 - kdgR b1827 0.46 -0.1 1,908,131 - +0.4 1,908,133 - +0.4 1,908,151 - -0.0
Or see this region's nucleotide sequence