Experiment: LB Anaerobic with Zinc Pyrithione 0.00000125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rrsG and clpB are separated by 442 nucleotides clpB and ryfD overlap by 21 nucleotides ryfD and yfiH are separated by 7 nucleotides yfiH and rluD overlap by 4 nucleotides
b2591: rrsG - 16S ribosomal RNA (NCBI), at 2,727,638 to 2,729,179
rrsG
b2592: clpB - protein disaggregation chaperone (NCBI), at 2,729,622 to 2,732,195
clpB
b4609: ryfD - Novel sRNA, function unknown (RefSeq), at 2,732,175 to 2,732,317
ryfD
b2593: yfiH - hypothetical protein (NCBI), at 2,732,325 to 2,733,056
yfiH
b2594: rluD - 23S rRNA pseudouridine synthase (NCBI), at 2,733,053 to 2,734,033
rluD
Position (kb)
2729
2730
2731
2732
2733 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2729.412 kb on - strand at 2729.705 kb on - strand at 2729.753 kb on + strand at 2729.753 kb on + strand at 2729.761 kb on - strand at 2729.900 kb on + strand, within clpB at 2730.084 kb on + strand, within clpB at 2730.092 kb on - strand, within clpB at 2730.173 kb on + strand, within clpB at 2730.189 kb on - strand, within clpB at 2730.269 kb on - strand, within clpB at 2730.269 kb on - strand, within clpB at 2730.304 kb on - strand, within clpB at 2730.304 kb on - strand, within clpB at 2730.346 kb on + strand, within clpB at 2730.357 kb on - strand, within clpB at 2730.357 kb on - strand, within clpB at 2730.392 kb on + strand, within clpB at 2730.456 kb on - strand, within clpB at 2730.516 kb on - strand, within clpB at 2730.565 kb on + strand, within clpB at 2730.755 kb on + strand, within clpB at 2730.787 kb on - strand, within clpB at 2730.798 kb on + strand, within clpB at 2730.798 kb on + strand, within clpB at 2730.862 kb on - strand, within clpB at 2730.901 kb on - strand, within clpB at 2730.901 kb on - strand, within clpB at 2730.902 kb on + strand, within clpB at 2731.039 kb on + strand, within clpB at 2731.048 kb on - strand, within clpB at 2731.121 kb on - strand, within clpB at 2731.121 kb on - strand, within clpB at 2731.133 kb on + strand, within clpB at 2731.156 kb on - strand, within clpB at 2731.267 kb on - strand, within clpB at 2731.267 kb on - strand, within clpB at 2731.351 kb on + strand, within clpB at 2731.448 kb on - strand, within clpB at 2731.468 kb on + strand, within clpB at 2731.476 kb on - strand, within clpB at 2731.476 kb on - strand, within clpB at 2731.481 kb on - strand, within clpB at 2731.531 kb on - strand, within clpB at 2731.575 kb on - strand, within clpB at 2731.674 kb on - strand, within clpB at 2731.730 kb on + strand, within clpB at 2731.736 kb on - strand, within clpB at 2731.746 kb on - strand, within clpB at 2731.746 kb on - strand, within clpB at 2731.857 kb on - strand, within clpB at 2731.868 kb on + strand, within clpB at 2731.919 kb on + strand, within clpB at 2731.927 kb on - strand, within clpB at 2731.965 kb on - strand at 2731.966 kb on - strand at 2731.966 kb on - strand at 2732.002 kb on - strand at 2732.140 kb on - strand at 2732.202 kb on + strand, within ryfD at 2732.205 kb on - strand, within ryfD at 2732.212 kb on + strand, within ryfD at 2732.212 kb on + strand, within ryfD at 2732.251 kb on + strand, within ryfD at 2732.277 kb on - strand, within ryfD at 2732.277 kb on - strand, within ryfD at 2732.323 kb on + strand at 2732.323 kb on + strand at 2732.456 kb on - strand, within yfiH at 2732.499 kb on - strand, within yfiH at 2732.499 kb on - strand, within yfiH at 2732.502 kb on - strand, within yfiH at 2732.502 kb on - strand, within yfiH at 2732.563 kb on - strand, within yfiH at 2732.563 kb on - strand, within yfiH at 2732.594 kb on + strand, within yfiH at 2732.602 kb on - strand, within yfiH at 2732.648 kb on + strand, within yfiH at 2732.659 kb on + strand, within yfiH at 2732.669 kb on + strand, within yfiH at 2732.748 kb on + strand, within yfiH at 2732.756 kb on - strand, within yfiH at 2732.763 kb on - strand, within yfiH at 2732.763 kb on - strand, within yfiH at 2732.778 kb on + strand, within yfiH at 2732.813 kb on - strand, within yfiH at 2732.813 kb on - strand, within yfiH at 2732.852 kb on - strand, within yfiH at 2732.854 kb on - strand, within yfiH at 2732.926 kb on + strand, within yfiH at 2732.943 kb on - strand, within yfiH at 2732.969 kb on - strand, within yfiH at 2732.969 kb on - strand, within yfiH at 2732.994 kb on + strand at 2733.052 kb on + strand at 2733.060 kb on - strand at 2733.062 kb on - strand at 2733.072 kb on + strand at 2733.079 kb on - strand at 2733.079 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Zinc Pyrithione 0.00000125 mM remove 2,729,412 - -2.6 2,729,705 - -0.1 2,729,753 + -0.7 2,729,753 + -1.0 2,729,761 - +0.6 2,729,900 + clpB b2592 0.11 +0.5 2,730,084 + clpB b2592 0.18 +0.7 2,730,092 - clpB b2592 0.18 +0.8 2,730,173 + clpB b2592 0.21 +0.4 2,730,189 - clpB b2592 0.22 +0.2 2,730,269 - clpB b2592 0.25 -0.1 2,730,269 - clpB b2592 0.25 +0.1 2,730,304 - clpB b2592 0.26 +0.1 2,730,304 - clpB b2592 0.26 +0.4 2,730,346 + clpB b2592 0.28 +0.2 2,730,357 - clpB b2592 0.29 +0.6 2,730,357 - clpB b2592 0.29 +0.1 2,730,392 + clpB b2592 0.30 +1.2 2,730,456 - clpB b2592 0.32 +0.6 2,730,516 - clpB b2592 0.35 +0.1 2,730,565 + clpB b2592 0.37 -0.2 2,730,755 + clpB b2592 0.44 -0.7 2,730,787 - clpB b2592 0.45 -0.0 2,730,798 + clpB b2592 0.46 -1.0 2,730,798 + clpB b2592 0.46 +0.6 2,730,862 - clpB b2592 0.48 +0.6 2,730,901 - clpB b2592 0.50 -1.0 2,730,901 - clpB b2592 0.50 +1.8 2,730,902 + clpB b2592 0.50 +0.3 2,731,039 + clpB b2592 0.55 -0.2 2,731,048 - clpB b2592 0.55 +0.8 2,731,121 - clpB b2592 0.58 -1.2 2,731,121 - clpB b2592 0.58 +0.2 2,731,133 + clpB b2592 0.59 +0.6 2,731,156 - clpB b2592 0.60 +0.6 2,731,267 - clpB b2592 0.64 +0.4 2,731,267 - clpB b2592 0.64 -0.8 2,731,351 + clpB b2592 0.67 -0.8 2,731,448 - clpB b2592 0.71 -1.7 2,731,468 + clpB b2592 0.72 -0.2 2,731,476 - clpB b2592 0.72 -0.5 2,731,476 - clpB b2592 0.72 -1.1 2,731,481 - clpB b2592 0.72 +0.9 2,731,531 - clpB b2592 0.74 -0.3 2,731,575 - clpB b2592 0.76 +0.2 2,731,674 - clpB b2592 0.80 -1.1 2,731,730 + clpB b2592 0.82 +0.3 2,731,736 - clpB b2592 0.82 +1.6 2,731,746 - clpB b2592 0.83 +0.3 2,731,746 - clpB b2592 0.83 +1.8 2,731,857 - clpB b2592 0.87 +0.3 2,731,868 + clpB b2592 0.87 +0.3 2,731,919 + clpB b2592 0.89 -0.1 2,731,927 - clpB b2592 0.90 +0.1 2,731,965 - -0.6 2,731,966 - -1.4 2,731,966 - +0.6 2,732,002 - +1.6 2,732,140 - +1.3 2,732,202 + ryfD b4609 0.19 -0.8 2,732,205 - ryfD b4609 0.21 +0.5 2,732,212 + ryfD b4609 0.26 -0.9 2,732,212 + ryfD b4609 0.26 -1.0 2,732,251 + ryfD b4609 0.53 +0.0 2,732,277 - ryfD b4609 0.71 +0.6 2,732,277 - ryfD b4609 0.71 -0.2 2,732,323 + +1.8 2,732,323 + -1.0 2,732,456 - yfiH b2593 0.18 -0.6 2,732,499 - yfiH b2593 0.24 -0.3 2,732,499 - yfiH b2593 0.24 +0.1 2,732,502 - yfiH b2593 0.24 +0.6 2,732,502 - yfiH b2593 0.24 -1.0 2,732,563 - yfiH b2593 0.33 +0.1 2,732,563 - yfiH b2593 0.33 -1.1 2,732,594 + yfiH b2593 0.37 -0.6 2,732,602 - yfiH b2593 0.38 -0.1 2,732,648 + yfiH b2593 0.44 -0.3 2,732,659 + yfiH b2593 0.46 -0.6 2,732,669 + yfiH b2593 0.47 +0.6 2,732,748 + yfiH b2593 0.58 -0.4 2,732,756 - yfiH b2593 0.59 +0.5 2,732,763 - yfiH b2593 0.60 -0.6 2,732,763 - yfiH b2593 0.60 +0.7 2,732,778 + yfiH b2593 0.62 +0.5 2,732,813 - yfiH b2593 0.67 -0.1 2,732,813 - yfiH b2593 0.67 -0.1 2,732,852 - yfiH b2593 0.72 +0.2 2,732,854 - yfiH b2593 0.72 +0.1 2,732,926 + yfiH b2593 0.82 +0.3 2,732,943 - yfiH b2593 0.84 +0.1 2,732,969 - yfiH b2593 0.88 +0.2 2,732,969 - yfiH b2593 0.88 -0.4 2,732,994 + +0.5 2,733,052 + -0.2 2,733,060 - -0.4 2,733,062 - +0.3 2,733,072 + -1.0 2,733,079 - -0.9 2,733,079 - -0.3
Or see this region's nucleotide sequence