Strain Fitness in Escherichia coli BW25113 around b2254

Experiment: LB Anaerobic with Chlorhexidine diacetate salt 0.000000625 mM

Add experiment(s):

Zoom: Pan:

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

download SVG
500 ntarnB and yfbF are separated by 3 nucleotidesyfbF and yfbG overlap by 1 nucleotides b2253: arnB - uridine 5'-(beta-1-threo-pentapyranosyl-4-ulose diphosphate) aminotransferase, PLP-dependent (RefSeq), at 2,363,950 to 2,365,089 arnB b2254: yfbF - undecaprenyl phosphate-L-Ara4FN transferase (NCBI), at 2,365,093 to 2,366,061 yfbF b2255: yfbG - hypothetical protein (NCBI), at 2,366,061 to 2,368,043 yfbG Position (kb) 2365 2366 2367Strain fitness (log2 ratio) -2 -1 0 1 2at 2364.168 kb on - strand, within arnBat 2364.168 kb on - strand, within arnBat 2364.185 kb on - strand, within arnBat 2364.192 kb on + strand, within arnBat 2364.195 kb on - strand, within arnBat 2364.244 kb on - strand, within arnBat 2364.301 kb on + strand, within arnBat 2364.314 kb on + strand, within arnBat 2364.333 kb on + strand, within arnBat 2364.514 kb on - strand, within arnBat 2364.522 kb on + strand, within arnBat 2364.590 kb on - strand, within arnBat 2364.590 kb on - strand, within arnBat 2364.751 kb on - strand, within arnBat 2364.796 kb on - strand, within arnBat 2364.796 kb on - strand, within arnBat 2364.815 kb on - strand, within arnBat 2364.844 kb on + strand, within arnBat 2364.844 kb on + strand, within arnBat 2364.857 kb on - strand, within arnBat 2364.863 kb on + strand, within arnBat 2364.863 kb on + strand, within arnBat 2364.918 kb on - strand, within arnBat 2364.926 kb on + strand, within arnBat 2364.926 kb on + strand, within arnBat 2365.002 kb on + strandat 2365.021 kb on + strandat 2365.035 kb on - strandat 2365.053 kb on + strandat 2365.053 kb on + strandat 2365.056 kb on + strandat 2365.179 kb on - strandat 2365.181 kb on + strandat 2365.198 kb on - strand, within yfbFat 2365.200 kb on + strand, within yfbFat 2365.208 kb on - strand, within yfbFat 2365.220 kb on - strand, within yfbFat 2365.264 kb on - strand, within yfbFat 2365.290 kb on - strand, within yfbFat 2365.290 kb on - strand, within yfbFat 2365.414 kb on + strand, within yfbFat 2365.433 kb on + strand, within yfbFat 2365.450 kb on + strand, within yfbFat 2365.477 kb on - strand, within yfbFat 2365.529 kb on + strand, within yfbFat 2365.555 kb on + strand, within yfbFat 2365.555 kb on + strand, within yfbFat 2365.637 kb on + strand, within yfbFat 2365.773 kb on + strand, within yfbFat 2365.786 kb on - strand, within yfbFat 2365.786 kb on - strand, within yfbFat 2365.804 kb on - strand, within yfbFat 2365.854 kb on - strand, within yfbFat 2365.866 kb on + strand, within yfbFat 2365.866 kb on + strand, within yfbFat 2365.965 kb on - strandat 2365.983 kb on + strandat 2365.984 kb on + strandat 2365.984 kb on + strandat 2366.037 kb on - strandat 2366.098 kb on + strandat 2366.245 kb on - strandat 2366.255 kb on + strandat 2366.268 kb on - strand, within yfbGat 2366.274 kb on + strand, within yfbGat 2366.279 kb on + strand, within yfbGat 2366.280 kb on - strand, within yfbGat 2366.280 kb on - strand, within yfbGat 2366.364 kb on + strand, within yfbGat 2366.381 kb on - strand, within yfbGat 2366.541 kb on - strand, within yfbGat 2366.541 kb on - strand, within yfbGat 2366.553 kb on - strand, within yfbGat 2366.553 kb on - strand, within yfbGat 2366.578 kb on - strand, within yfbGat 2366.578 kb on - strand, within yfbGat 2366.580 kb on + strand, within yfbGat 2366.667 kb on - strand, within yfbGat 2366.714 kb on + strand, within yfbGat 2366.758 kb on + strand, within yfbGat 2366.820 kb on + strand, within yfbGat 2366.912 kb on - strand, within yfbGat 2366.924 kb on - strand, within yfbGat 2366.963 kb on - strand, within yfbGat 2366.970 kb on - strand, within yfbGat 2366.984 kb on + strand, within yfbGat 2366.992 kb on - strand, within yfbG

download strain data

Per-strain Table

Position Strand Gene LocusTag Fraction LB Anaerobic with Chlorhexidine diacetate salt 0.000000625 mM
remove
2,364,168 - arnB b2253 0.19 +0.7
2,364,168 - arnB b2253 0.19 +1.0
2,364,185 - arnB b2253 0.21 +0.3
2,364,192 + arnB b2253 0.21 +1.5
2,364,195 - arnB b2253 0.21 -0.3
2,364,244 - arnB b2253 0.26 +1.3
2,364,301 + arnB b2253 0.31 +0.6
2,364,314 + arnB b2253 0.32 -0.5
2,364,333 + arnB b2253 0.34 +0.3
2,364,514 - arnB b2253 0.49 +0.5
2,364,522 + arnB b2253 0.50 -1.5
2,364,590 - arnB b2253 0.56 +0.3
2,364,590 - arnB b2253 0.56 -0.1
2,364,751 - arnB b2253 0.70 +0.4
2,364,796 - arnB b2253 0.74 -1.1
2,364,796 - arnB b2253 0.74 +0.5
2,364,815 - arnB b2253 0.76 +0.5
2,364,844 + arnB b2253 0.78 -0.2
2,364,844 + arnB b2253 0.78 -1.5
2,364,857 - arnB b2253 0.80 -0.1
2,364,863 + arnB b2253 0.80 -0.2
2,364,863 + arnB b2253 0.80 -0.1
2,364,918 - arnB b2253 0.85 +0.3
2,364,926 + arnB b2253 0.86 -2.3
2,364,926 + arnB b2253 0.86 -0.8
2,365,002 + -0.8
2,365,021 + +0.1
2,365,035 - +0.6
2,365,053 + -0.6
2,365,053 + +0.4
2,365,056 + +2.6
2,365,179 - -0.2
2,365,181 + +2.3
2,365,198 - yfbF b2254 0.11 -0.1
2,365,200 + yfbF b2254 0.11 -1.0
2,365,208 - yfbF b2254 0.12 -0.3
2,365,220 - yfbF b2254 0.13 +1.3
2,365,264 - yfbF b2254 0.18 +0.4
2,365,290 - yfbF b2254 0.20 -0.3
2,365,290 - yfbF b2254 0.20 +0.1
2,365,414 + yfbF b2254 0.33 -0.5
2,365,433 + yfbF b2254 0.35 +0.0
2,365,450 + yfbF b2254 0.37 +0.1
2,365,477 - yfbF b2254 0.40 -1.1
2,365,529 + yfbF b2254 0.45 +1.1
2,365,555 + yfbF b2254 0.48 -0.2
2,365,555 + yfbF b2254 0.48 +0.1
2,365,637 + yfbF b2254 0.56 -1.1
2,365,773 + yfbF b2254 0.70 -0.3
2,365,786 - yfbF b2254 0.72 -1.9
2,365,786 - yfbF b2254 0.72 +1.3
2,365,804 - yfbF b2254 0.73 -0.6
2,365,854 - yfbF b2254 0.79 -0.2
2,365,866 + yfbF b2254 0.80 -0.9
2,365,866 + yfbF b2254 0.80 -1.4
2,365,965 - +0.5
2,365,983 + +0.2
2,365,984 + +0.2
2,365,984 + -0.7
2,366,037 - +2.4
2,366,098 + +1.6
2,366,245 - -1.8
2,366,255 + +0.0
2,366,268 - yfbG b2255 0.10 +1.5
2,366,274 + yfbG b2255 0.11 +0.1
2,366,279 + yfbG b2255 0.11 +0.4
2,366,280 - yfbG b2255 0.11 -2.5
2,366,280 - yfbG b2255 0.11 -2.7
2,366,364 + yfbG b2255 0.15 -0.8
2,366,381 - yfbG b2255 0.16 +0.3
2,366,541 - yfbG b2255 0.24 +0.7
2,366,541 - yfbG b2255 0.24 +0.8
2,366,553 - yfbG b2255 0.25 +1.2
2,366,553 - yfbG b2255 0.25 -0.8
2,366,578 - yfbG b2255 0.26 -2.5
2,366,578 - yfbG b2255 0.26 +0.1
2,366,580 + yfbG b2255 0.26 +0.4
2,366,667 - yfbG b2255 0.31 -1.4
2,366,714 + yfbG b2255 0.33 +0.6
2,366,758 + yfbG b2255 0.35 +1.0
2,366,820 + yfbG b2255 0.38 +1.4
2,366,912 - yfbG b2255 0.43 +0.1
2,366,924 - yfbG b2255 0.44 +1.8
2,366,963 - yfbG b2255 0.45 -0.7
2,366,970 - yfbG b2255 0.46 +0.6
2,366,984 + yfbG b2255 0.47 +0.5
2,366,992 - yfbG b2255 0.47 -0.6

Or see this region's nucleotide sequence