Strain Fitness in Escherichia coli BW25113 around b1247

Experiment: LB Anaerobic with Chlorhexidine diacetate salt 0.000000625 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntoppD and oppF overlap by 4 nucleotidesoppF and b1248 are separated by 52 nucleotidesb1248 and cls are separated by 34 nucleotides b1246: oppD - oligopeptide transporter ATP-binding component (NCBI), at 1,302,778 to 1,303,791 oppD b1247: oppF - oligopeptide transporter subunit (NCBI), at 1,303,788 to 1,304,792 oppF b1248: b1248 - orf, hypothetical protein (VIMSS), at 1,304,845 to 1,305,174 b1248 b1249: cls - cardiolipin synthetase (NCBI), at 1,305,209 to 1,306,669 cls Position (kb) 1303 1304 1305Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 1302.812 kb on + strandat 1302.812 kb on + strandat 1302.860 kb on + strandat 1302.933 kb on + strand, within oppDat 1303.045 kb on + strand, within oppDat 1303.071 kb on + strand, within oppDat 1303.132 kb on + strand, within oppDat 1303.142 kb on + strand, within oppDat 1303.155 kb on + strand, within oppDat 1303.165 kb on + strand, within oppDat 1303.201 kb on - strand, within oppDat 1303.342 kb on - strand, within oppDat 1303.359 kb on + strand, within oppDat 1303.403 kb on - strand, within oppDat 1303.405 kb on + strand, within oppDat 1303.434 kb on - strand, within oppDat 1303.439 kb on + strand, within oppDat 1303.439 kb on + strand, within oppDat 1303.517 kb on + strand, within oppDat 1303.563 kb on + strand, within oppDat 1303.571 kb on - strand, within oppDat 1303.902 kb on + strand, within oppFat 1303.914 kb on - strand, within oppFat 1303.981 kb on + strand, within oppFat 1303.981 kb on + strand, within oppFat 1304.158 kb on + strand, within oppFat 1304.294 kb on + strand, within oppFat 1304.294 kb on + strand, within oppFat 1304.367 kb on + strand, within oppFat 1304.720 kb on + strandat 1304.780 kb on - strandat 1304.780 kb on - strandat 1305.078 kb on - strand, within b1248at 1305.078 kb on - strand, within b1248at 1305.176 kb on + strandat 1305.186 kb on + strandat 1305.539 kb on + strand, within clsat 1305.539 kb on + strand, within clsat 1305.576 kb on - strand, within clsat 1305.588 kb on - strand, within clsat 1305.588 kb on - strand, within clsat 1305.625 kb on - strand, within clsat 1305.641 kb on + strand, within clsat 1305.641 kb on + strand, within cls

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Anaerobic with Chlorhexidine diacetate salt 0.000000625 mM
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1,302,812 + +0.6
1,302,812 + +0.3
1,302,860 + +0.9
1,302,933 + oppD b1246 0.15 -0.1
1,303,045 + oppD b1246 0.26 +0.4
1,303,071 + oppD b1246 0.29 +1.2
1,303,132 + oppD b1246 0.35 +0.5
1,303,142 + oppD b1246 0.36 +0.1
1,303,155 + oppD b1246 0.37 +0.4
1,303,165 + oppD b1246 0.38 +1.4
1,303,201 - oppD b1246 0.42 +0.1
1,303,342 - oppD b1246 0.56 -1.1
1,303,359 + oppD b1246 0.57 +0.7
1,303,403 - oppD b1246 0.62 +0.5
1,303,405 + oppD b1246 0.62 +0.7
1,303,434 - oppD b1246 0.65 +2.1
1,303,439 + oppD b1246 0.65 +0.7
1,303,439 + oppD b1246 0.65 -1.8
1,303,517 + oppD b1246 0.73 +0.4
1,303,563 + oppD b1246 0.77 +0.0
1,303,571 - oppD b1246 0.78 +0.2
1,303,902 + oppF b1247 0.11 +0.3
1,303,914 - oppF b1247 0.13 +0.1
1,303,981 + oppF b1247 0.19 +0.1
1,303,981 + oppF b1247 0.19 +1.4
1,304,158 + oppF b1247 0.37 +0.3
1,304,294 + oppF b1247 0.50 +2.2
1,304,294 + oppF b1247 0.50 +0.6
1,304,367 + oppF b1247 0.58 +1.3
1,304,720 + -0.9
1,304,780 - -0.3
1,304,780 - +1.1
1,305,078 - b1248 b1248 0.71 +0.8
1,305,078 - b1248 b1248 0.71 -1.3
1,305,176 + -2.3
1,305,186 + +1.0
1,305,539 + cls b1249 0.23 -1.1
1,305,539 + cls b1249 0.23 -1.1
1,305,576 - cls b1249 0.25 -2.3
1,305,588 - cls b1249 0.26 -3.0
1,305,588 - cls b1249 0.26 -1.8
1,305,625 - cls b1249 0.28 -3.5
1,305,641 + cls b1249 0.30 -1.8
1,305,641 + cls b1249 0.30 -1.5

Or see this region's nucleotide sequence