Experiment: KB with Avocadene 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_3005 and Psyr_3006 are separated by 126 nucleotides Psyr_3006 and Psyr_3007 overlap by 6 nucleotides Psyr_3007 and Psyr_3008 overlap by 4 nucleotides Psyr_3008 and Psyr_3009 are separated by 251 nucleotides Psyr_3009 and Psyr_3010 overlap by 4 nucleotides
Psyr_3005: Psyr_3005 - 3-oxoadipate enol-lactonase, at 3,600,896 to 3,601,687
_3005
Psyr_3006: Psyr_3006 - Protein of unknown function DUF419, at 3,601,814 to 3,602,182
_3006
Psyr_3007: Psyr_3007 - Protein of unknown function DUF1294, at 3,602,177 to 3,602,611
_3007
Psyr_3008: Psyr_3008 - Undecaprenyl-diphosphatase, at 3,602,608 to 3,603,438
_3008
Psyr_3009: Psyr_3009 - Nicotinamide mononucleotide transporter PnuC, at 3,603,690 to 3,604,253
_3009
Psyr_3010: Psyr_3010 - nicotinamide-nucleotide adenylyltransferase, putative, at 3,604,250 to 3,604,795
_3010
Position (kb)
3602
3603
3604 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 3601.650 kb on + strand at 3601.651 kb on - strand at 3601.720 kb on + strand at 3601.721 kb on - strand at 3601.740 kb on + strand at 3601.741 kb on - strand at 3601.741 kb on - strand at 3601.741 kb on - strand at 3601.741 kb on - strand at 3601.741 kb on - strand at 3601.741 kb on - strand at 3601.741 kb on - strand at 3601.741 kb on - strand at 3601.741 kb on - strand at 3601.895 kb on + strand, within Psyr_3006 at 3601.895 kb on + strand, within Psyr_3006 at 3601.895 kb on + strand, within Psyr_3006 at 3601.895 kb on + strand, within Psyr_3006 at 3602.028 kb on + strand, within Psyr_3006 at 3602.043 kb on - strand, within Psyr_3006 at 3602.043 kb on - strand, within Psyr_3006 at 3602.079 kb on + strand, within Psyr_3006 at 3602.079 kb on + strand, within Psyr_3006 at 3602.079 kb on + strand, within Psyr_3006 at 3602.080 kb on - strand, within Psyr_3006 at 3602.178 kb on + strand at 3602.178 kb on + strand at 3602.178 kb on + strand at 3602.179 kb on - strand at 3602.179 kb on - strand at 3602.179 kb on - strand at 3602.264 kb on + strand, within Psyr_3007 at 3602.677 kb on + strand at 3602.678 kb on - strand at 3602.702 kb on + strand, within Psyr_3008 at 3602.703 kb on - strand, within Psyr_3008 at 3602.766 kb on + strand, within Psyr_3008 at 3602.820 kb on - strand, within Psyr_3008 at 3602.834 kb on + strand, within Psyr_3008 at 3602.974 kb on + strand, within Psyr_3008 at 3602.974 kb on + strand, within Psyr_3008 at 3602.975 kb on - strand, within Psyr_3008 at 3602.975 kb on - strand, within Psyr_3008 at 3602.984 kb on - strand, within Psyr_3008 at 3603.116 kb on + strand, within Psyr_3008 at 3603.117 kb on - strand, within Psyr_3008 at 3603.117 kb on - strand, within Psyr_3008 at 3603.263 kb on - strand, within Psyr_3008 at 3603.394 kb on + strand at 3603.394 kb on + strand at 3603.395 kb on - strand at 3603.395 kb on - strand at 3603.506 kb on - strand at 3603.506 kb on - strand at 3603.513 kb on + strand at 3603.513 kb on + strand at 3603.568 kb on - strand at 3603.568 kb on - strand at 3603.599 kb on + strand at 3603.599 kb on + strand at 3603.599 kb on + strand at 3603.599 kb on + strand at 3603.599 kb on + strand at 3603.600 kb on - strand at 3603.600 kb on - strand at 3603.600 kb on - strand at 3603.644 kb on + strand at 3603.644 kb on + strand at 3603.644 kb on + strand at 3603.645 kb on - strand at 3603.798 kb on + strand, within Psyr_3009 at 3603.798 kb on + strand, within Psyr_3009 at 3603.799 kb on - strand, within Psyr_3009 at 3603.799 kb on - strand, within Psyr_3009 at 3603.799 kb on - strand, within Psyr_3009 at 3603.799 kb on - strand, within Psyr_3009 at 3603.799 kb on - strand, within Psyr_3009 at 3603.799 kb on - strand, within Psyr_3009 at 3603.799 kb on - strand, within Psyr_3009 at 3603.809 kb on - strand, within Psyr_3009 at 3603.873 kb on + strand, within Psyr_3009 at 3603.873 kb on + strand, within Psyr_3009 at 3603.873 kb on + strand, within Psyr_3009 at 3603.874 kb on - strand, within Psyr_3009 at 3603.874 kb on - strand, within Psyr_3009 at 3604.152 kb on + strand, within Psyr_3009 at 3604.152 kb on + strand, within Psyr_3009 at 3604.152 kb on + strand, within Psyr_3009 at 3604.153 kb on - strand, within Psyr_3009 at 3604.153 kb on - strand, within Psyr_3009 at 3604.164 kb on + strand, within Psyr_3009 at 3604.165 kb on - strand, within Psyr_3009 at 3604.165 kb on - strand, within Psyr_3009 at 3604.165 kb on - strand, within Psyr_3009 at 3604.182 kb on + strand, within Psyr_3009 at 3604.183 kb on - strand, within Psyr_3009 at 3604.311 kb on + strand, within Psyr_3010 at 3604.311 kb on + strand, within Psyr_3010 at 3604.312 kb on - strand, within Psyr_3010 at 3604.312 kb on - strand, within Psyr_3010 at 3604.318 kb on + strand, within Psyr_3010 at 3604.318 kb on + strand, within Psyr_3010 at 3604.318 kb on + strand, within Psyr_3010 at 3604.319 kb on - strand, within Psyr_3010 at 3604.319 kb on - strand, within Psyr_3010 at 3604.319 kb on - strand, within Psyr_3010 at 3604.319 kb on - strand, within Psyr_3010 at 3604.319 kb on - strand, within Psyr_3010 at 3604.319 kb on - strand, within Psyr_3010 at 3604.319 kb on - strand, within Psyr_3010 at 3604.346 kb on + strand, within Psyr_3010 at 3604.398 kb on + strand, within Psyr_3010
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Avocadene 10 mM remove 3,601,650 + -0.3 3,601,651 - +0.1 3,601,720 + +0.3 3,601,721 - +0.6 3,601,740 + -0.1 3,601,741 - +0.1 3,601,741 - +0.1 3,601,741 - +1.8 3,601,741 - +0.1 3,601,741 - +0.1 3,601,741 - -0.4 3,601,741 - -0.6 3,601,741 - -0.4 3,601,741 - -0.5 3,601,895 + Psyr_3006 0.22 +3.3 3,601,895 + Psyr_3006 0.22 +0.1 3,601,895 + Psyr_3006 0.22 +0.0 3,601,895 + Psyr_3006 0.22 +0.6 3,602,028 + Psyr_3006 0.58 -0.5 3,602,043 - Psyr_3006 0.62 -0.1 3,602,043 - Psyr_3006 0.62 -1.4 3,602,079 + Psyr_3006 0.72 -0.5 3,602,079 + Psyr_3006 0.72 +0.1 3,602,079 + Psyr_3006 0.72 -2.4 3,602,080 - Psyr_3006 0.72 +0.9 3,602,178 + -0.5 3,602,178 + +0.4 3,602,178 + -1.1 3,602,179 - +1.9 3,602,179 - +0.3 3,602,179 - +0.5 3,602,264 + Psyr_3007 0.20 +1.7 3,602,677 + -1.4 3,602,678 - -3.1 3,602,702 + Psyr_3008 0.11 -2.2 3,602,703 - Psyr_3008 0.11 -3.4 3,602,766 + Psyr_3008 0.19 -1.4 3,602,820 - Psyr_3008 0.26 -3.5 3,602,834 + Psyr_3008 0.27 -1.4 3,602,974 + Psyr_3008 0.44 -3.9 3,602,974 + Psyr_3008 0.44 -2.6 3,602,975 - Psyr_3008 0.44 -0.1 3,602,975 - Psyr_3008 0.44 -2.6 3,602,984 - Psyr_3008 0.45 -2.6 3,603,116 + Psyr_3008 0.61 -2.4 3,603,117 - Psyr_3008 0.61 -3.5 3,603,117 - Psyr_3008 0.61 -2.9 3,603,263 - Psyr_3008 0.79 -3.0 3,603,394 + -2.2 3,603,394 + -1.4 3,603,395 - -1.4 3,603,395 - -3.0 3,603,506 - -1.1 3,603,506 - -0.2 3,603,513 + -3.0 3,603,513 + -2.2 3,603,568 - +0.8 3,603,568 - +1.1 3,603,599 + +0.9 3,603,599 + -0.9 3,603,599 + +0.5 3,603,599 + +1.9 3,603,599 + +0.6 3,603,600 - -0.1 3,603,600 - +0.2 3,603,600 - +0.1 3,603,644 + -0.3 3,603,644 + +0.9 3,603,644 + +0.3 3,603,645 - +0.5 3,603,798 + Psyr_3009 0.19 +0.3 3,603,798 + Psyr_3009 0.19 -1.0 3,603,799 - Psyr_3009 0.19 +1.4 3,603,799 - Psyr_3009 0.19 +0.9 3,603,799 - Psyr_3009 0.19 +0.3 3,603,799 - Psyr_3009 0.19 -0.9 3,603,799 - Psyr_3009 0.19 -0.3 3,603,799 - Psyr_3009 0.19 +0.5 3,603,799 - Psyr_3009 0.19 -0.9 3,603,809 - Psyr_3009 0.21 -0.3 3,603,873 + Psyr_3009 0.32 +0.3 3,603,873 + Psyr_3009 0.32 -0.9 3,603,873 + Psyr_3009 0.32 -0.1 3,603,874 - Psyr_3009 0.33 +0.1 3,603,874 - Psyr_3009 0.33 +0.6 3,604,152 + Psyr_3009 0.82 -0.2 3,604,152 + Psyr_3009 0.82 +1.9 3,604,152 + Psyr_3009 0.82 +0.6 3,604,153 - Psyr_3009 0.82 -2.4 3,604,153 - Psyr_3009 0.82 -0.2 3,604,164 + Psyr_3009 0.84 -0.9 3,604,165 - Psyr_3009 0.84 +0.1 3,604,165 - Psyr_3009 0.84 +1.5 3,604,165 - Psyr_3009 0.84 -0.6 3,604,182 + Psyr_3009 0.87 +0.2 3,604,183 - Psyr_3009 0.87 -0.6 3,604,311 + Psyr_3010 0.11 +0.4 3,604,311 + Psyr_3010 0.11 +0.6 3,604,312 - Psyr_3010 0.11 +0.2 3,604,312 - Psyr_3010 0.11 -3.0 3,604,318 + Psyr_3010 0.12 +0.6 3,604,318 + Psyr_3010 0.12 -0.6 3,604,318 + Psyr_3010 0.12 -0.1 3,604,319 - Psyr_3010 0.13 -0.4 3,604,319 - Psyr_3010 0.13 -0.2 3,604,319 - Psyr_3010 0.13 -0.2 3,604,319 - Psyr_3010 0.13 -0.1 3,604,319 - Psyr_3010 0.13 -1.6 3,604,319 - Psyr_3010 0.13 -1.9 3,604,319 - Psyr_3010 0.13 -0.6 3,604,346 + Psyr_3010 0.18 -0.5 3,604,398 + Psyr_3010 0.27 -0.3
Or see this region's nucleotide sequence