Experiment: KB with Avocadene 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2753 and Psyr_2754 are separated by 49 nucleotides Psyr_2754 and Psyr_2755 are separated by 135 nucleotides Psyr_2755 and Psyr_2756 are separated by 101 nucleotides Psyr_2756 and Psyr_2757 are separated by 0 nucleotides
Psyr_2753: Psyr_2753 - Zinc-containing alcohol dehydrogenase superfamily, at 3,341,164 to 3,342,147
_2753
Psyr_2754: Psyr_2754 - regulatory protein, TetR, at 3,342,197 to 3,342,856
_2754
Psyr_2755: Psyr_2755 - 2-nitropropane dioxygenase, NPD, at 3,342,992 to 3,344,071
_2755
Psyr_2756: Psyr_2756 - Molybdenum ABC transporter, periplasmic binding protein, at 3,344,173 to 3,344,940
_2756
Psyr_2757: Psyr_2757 - Binding-protein-dependent transport systems inner membrane component, at 3,344,941 to 3,345,621
_2757
Position (kb)
3342
3343
3344
3345 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3342.023 kb on + strand, within Psyr_2753 at 3342.023 kb on + strand, within Psyr_2753 at 3342.024 kb on - strand, within Psyr_2753 at 3342.024 kb on - strand, within Psyr_2753 at 3342.147 kb on + strand at 3342.147 kb on + strand at 3342.148 kb on - strand at 3342.148 kb on - strand at 3342.248 kb on - strand at 3342.256 kb on + strand at 3342.484 kb on - strand, within Psyr_2754 at 3342.600 kb on + strand, within Psyr_2754 at 3342.645 kb on + strand, within Psyr_2754 at 3342.645 kb on + strand, within Psyr_2754 at 3342.645 kb on + strand, within Psyr_2754 at 3342.645 kb on + strand, within Psyr_2754 at 3342.646 kb on - strand, within Psyr_2754 at 3342.646 kb on - strand, within Psyr_2754 at 3342.646 kb on - strand, within Psyr_2754 at 3342.652 kb on - strand, within Psyr_2754 at 3342.652 kb on - strand, within Psyr_2754 at 3342.690 kb on + strand, within Psyr_2754 at 3342.690 kb on + strand, within Psyr_2754 at 3342.691 kb on - strand, within Psyr_2754 at 3342.691 kb on - strand, within Psyr_2754 at 3342.691 kb on - strand, within Psyr_2754 at 3342.691 kb on - strand, within Psyr_2754 at 3342.691 kb on - strand, within Psyr_2754 at 3343.167 kb on + strand, within Psyr_2755 at 3343.283 kb on + strand, within Psyr_2755 at 3343.284 kb on - strand, within Psyr_2755 at 3343.284 kb on - strand, within Psyr_2755 at 3343.286 kb on + strand, within Psyr_2755 at 3343.287 kb on - strand, within Psyr_2755 at 3343.287 kb on - strand, within Psyr_2755 at 3343.287 kb on - strand, within Psyr_2755 at 3343.691 kb on - strand, within Psyr_2755 at 3344.103 kb on - strand at 3344.103 kb on - strand at 3344.105 kb on - strand at 3344.106 kb on + strand at 3344.107 kb on - strand at 3344.107 kb on - strand at 3344.107 kb on - strand at 3344.110 kb on + strand at 3344.110 kb on + strand at 3344.110 kb on + strand at 3344.111 kb on - strand at 3344.114 kb on + strand at 3344.115 kb on - strand at 3344.116 kb on + strand at 3344.117 kb on - strand at 3344.184 kb on + strand at 3344.185 kb on - strand at 3344.185 kb on - strand at 3344.374 kb on + strand, within Psyr_2756 at 3344.374 kb on + strand, within Psyr_2756 at 3344.374 kb on + strand, within Psyr_2756 at 3344.374 kb on + strand, within Psyr_2756 at 3344.374 kb on + strand, within Psyr_2756 at 3344.374 kb on + strand, within Psyr_2756 at 3344.375 kb on - strand, within Psyr_2756 at 3344.375 kb on - strand, within Psyr_2756 at 3344.377 kb on - strand, within Psyr_2756 at 3344.481 kb on + strand, within Psyr_2756 at 3344.481 kb on + strand, within Psyr_2756 at 3344.481 kb on + strand, within Psyr_2756 at 3344.482 kb on - strand, within Psyr_2756 at 3344.486 kb on - strand, within Psyr_2756 at 3344.528 kb on - strand, within Psyr_2756 at 3344.528 kb on - strand, within Psyr_2756 at 3344.528 kb on - strand, within Psyr_2756 at 3344.549 kb on + strand, within Psyr_2756 at 3344.567 kb on - strand, within Psyr_2756 at 3344.755 kb on + strand, within Psyr_2756 at 3344.756 kb on - strand, within Psyr_2756 at 3344.938 kb on + strand at 3344.939 kb on - strand at 3344.939 kb on - strand at 3344.939 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Avocadene 10 mM remove 3,342,023 + Psyr_2753 0.87 +0.0 3,342,023 + Psyr_2753 0.87 -1.4 3,342,024 - Psyr_2753 0.87 +0.3 3,342,024 - Psyr_2753 0.87 +1.0 3,342,147 + -0.6 3,342,147 + -0.4 3,342,148 - +0.4 3,342,148 - -1.8 3,342,248 - +1.4 3,342,256 + +0.4 3,342,484 - Psyr_2754 0.43 -1.1 3,342,600 + Psyr_2754 0.61 +1.0 3,342,645 + Psyr_2754 0.68 -0.3 3,342,645 + Psyr_2754 0.68 +0.4 3,342,645 + Psyr_2754 0.68 -0.9 3,342,645 + Psyr_2754 0.68 +0.2 3,342,646 - Psyr_2754 0.68 -0.8 3,342,646 - Psyr_2754 0.68 -1.6 3,342,646 - Psyr_2754 0.68 -0.6 3,342,652 - Psyr_2754 0.69 -0.1 3,342,652 - Psyr_2754 0.69 +1.2 3,342,690 + Psyr_2754 0.75 +0.5 3,342,690 + Psyr_2754 0.75 -1.5 3,342,691 - Psyr_2754 0.75 +2.4 3,342,691 - Psyr_2754 0.75 -1.3 3,342,691 - Psyr_2754 0.75 +0.5 3,342,691 - Psyr_2754 0.75 +0.7 3,342,691 - Psyr_2754 0.75 +1.6 3,343,167 + Psyr_2755 0.16 -0.6 3,343,283 + Psyr_2755 0.27 +0.2 3,343,284 - Psyr_2755 0.27 +0.5 3,343,284 - Psyr_2755 0.27 +0.5 3,343,286 + Psyr_2755 0.27 +0.9 3,343,287 - Psyr_2755 0.27 -1.3 3,343,287 - Psyr_2755 0.27 -0.7 3,343,287 - Psyr_2755 0.27 +2.0 3,343,691 - Psyr_2755 0.65 +0.4 3,344,103 - +1.0 3,344,103 - -0.2 3,344,105 - +0.2 3,344,106 + +0.7 3,344,107 - -0.6 3,344,107 - -0.8 3,344,107 - +1.3 3,344,110 + +1.0 3,344,110 + +0.4 3,344,110 + +0.4 3,344,111 - -0.1 3,344,114 + -3.1 3,344,115 - +1.0 3,344,116 + +0.1 3,344,117 - -0.4 3,344,184 + -0.1 3,344,185 - -1.8 3,344,185 - +1.2 3,344,374 + Psyr_2756 0.26 -0.2 3,344,374 + Psyr_2756 0.26 -1.4 3,344,374 + Psyr_2756 0.26 -1.5 3,344,374 + Psyr_2756 0.26 -2.9 3,344,374 + Psyr_2756 0.26 -0.2 3,344,374 + Psyr_2756 0.26 -0.6 3,344,375 - Psyr_2756 0.26 +0.4 3,344,375 - Psyr_2756 0.26 -2.1 3,344,377 - Psyr_2756 0.27 +0.4 3,344,481 + Psyr_2756 0.40 +0.3 3,344,481 + Psyr_2756 0.40 -0.9 3,344,481 + Psyr_2756 0.40 +0.9 3,344,482 - Psyr_2756 0.40 +0.2 3,344,486 - Psyr_2756 0.41 +0.0 3,344,528 - Psyr_2756 0.46 -0.5 3,344,528 - Psyr_2756 0.46 -1.0 3,344,528 - Psyr_2756 0.46 +2.6 3,344,549 + Psyr_2756 0.49 -0.9 3,344,567 - Psyr_2756 0.51 -0.9 3,344,755 + Psyr_2756 0.76 -0.2 3,344,756 - Psyr_2756 0.76 -1.0 3,344,938 + +0.4 3,344,939 - -1.4 3,344,939 - +0.0 3,344,939 - +0.6
Or see this region's nucleotide sequence