Strain Fitness in Pseudomonas syringae pv. syringae B728a ΔmexB around Psyr_2755

Experiment: KB with Avocadene 10 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsyr_2753 and Psyr_2754 are separated by 49 nucleotidesPsyr_2754 and Psyr_2755 are separated by 135 nucleotidesPsyr_2755 and Psyr_2756 are separated by 101 nucleotidesPsyr_2756 and Psyr_2757 are separated by 0 nucleotides Psyr_2753: Psyr_2753 - Zinc-containing alcohol dehydrogenase superfamily, at 3,341,164 to 3,342,147 _2753 Psyr_2754: Psyr_2754 - regulatory protein, TetR, at 3,342,197 to 3,342,856 _2754 Psyr_2755: Psyr_2755 - 2-nitropropane dioxygenase, NPD, at 3,342,992 to 3,344,071 _2755 Psyr_2756: Psyr_2756 - Molybdenum ABC transporter, periplasmic binding protein, at 3,344,173 to 3,344,940 _2756 Psyr_2757: Psyr_2757 - Binding-protein-dependent transport systems inner membrane component, at 3,344,941 to 3,345,621 _2757 Position (kb) 3342 3343 3344 3345Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 3342.023 kb on + strand, within Psyr_2753at 3342.023 kb on + strand, within Psyr_2753at 3342.024 kb on - strand, within Psyr_2753at 3342.024 kb on - strand, within Psyr_2753at 3342.147 kb on + strandat 3342.147 kb on + strandat 3342.148 kb on - strandat 3342.148 kb on - strandat 3342.248 kb on - strandat 3342.256 kb on + strandat 3342.484 kb on - strand, within Psyr_2754at 3342.600 kb on + strand, within Psyr_2754at 3342.645 kb on + strand, within Psyr_2754at 3342.645 kb on + strand, within Psyr_2754at 3342.645 kb on + strand, within Psyr_2754at 3342.645 kb on + strand, within Psyr_2754at 3342.646 kb on - strand, within Psyr_2754at 3342.646 kb on - strand, within Psyr_2754at 3342.646 kb on - strand, within Psyr_2754at 3342.652 kb on - strand, within Psyr_2754at 3342.652 kb on - strand, within Psyr_2754at 3342.690 kb on + strand, within Psyr_2754at 3342.690 kb on + strand, within Psyr_2754at 3342.691 kb on - strand, within Psyr_2754at 3342.691 kb on - strand, within Psyr_2754at 3342.691 kb on - strand, within Psyr_2754at 3342.691 kb on - strand, within Psyr_2754at 3342.691 kb on - strand, within Psyr_2754at 3343.167 kb on + strand, within Psyr_2755at 3343.283 kb on + strand, within Psyr_2755at 3343.284 kb on - strand, within Psyr_2755at 3343.284 kb on - strand, within Psyr_2755at 3343.286 kb on + strand, within Psyr_2755at 3343.287 kb on - strand, within Psyr_2755at 3343.287 kb on - strand, within Psyr_2755at 3343.287 kb on - strand, within Psyr_2755at 3343.691 kb on - strand, within Psyr_2755at 3344.103 kb on - strandat 3344.103 kb on - strandat 3344.105 kb on - strandat 3344.106 kb on + strandat 3344.107 kb on - strandat 3344.107 kb on - strandat 3344.107 kb on - strandat 3344.110 kb on + strandat 3344.110 kb on + strandat 3344.110 kb on + strandat 3344.111 kb on - strandat 3344.114 kb on + strandat 3344.115 kb on - strandat 3344.116 kb on + strandat 3344.117 kb on - strandat 3344.184 kb on + strandat 3344.185 kb on - strandat 3344.185 kb on - strandat 3344.374 kb on + strand, within Psyr_2756at 3344.374 kb on + strand, within Psyr_2756at 3344.374 kb on + strand, within Psyr_2756at 3344.374 kb on + strand, within Psyr_2756at 3344.374 kb on + strand, within Psyr_2756at 3344.374 kb on + strand, within Psyr_2756at 3344.375 kb on - strand, within Psyr_2756at 3344.375 kb on - strand, within Psyr_2756at 3344.377 kb on - strand, within Psyr_2756at 3344.481 kb on + strand, within Psyr_2756at 3344.481 kb on + strand, within Psyr_2756at 3344.481 kb on + strand, within Psyr_2756at 3344.482 kb on - strand, within Psyr_2756at 3344.486 kb on - strand, within Psyr_2756at 3344.528 kb on - strand, within Psyr_2756at 3344.528 kb on - strand, within Psyr_2756at 3344.528 kb on - strand, within Psyr_2756at 3344.549 kb on + strand, within Psyr_2756at 3344.567 kb on - strand, within Psyr_2756at 3344.755 kb on + strand, within Psyr_2756at 3344.756 kb on - strand, within Psyr_2756at 3344.938 kb on + strandat 3344.939 kb on - strandat 3344.939 kb on - strandat 3344.939 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction KB with Avocadene 10 mM
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3,342,023 + Psyr_2753 0.87 +0.0
3,342,023 + Psyr_2753 0.87 -1.4
3,342,024 - Psyr_2753 0.87 +0.3
3,342,024 - Psyr_2753 0.87 +1.0
3,342,147 + -0.6
3,342,147 + -0.4
3,342,148 - +0.4
3,342,148 - -1.8
3,342,248 - +1.4
3,342,256 + +0.4
3,342,484 - Psyr_2754 0.43 -1.1
3,342,600 + Psyr_2754 0.61 +1.0
3,342,645 + Psyr_2754 0.68 -0.3
3,342,645 + Psyr_2754 0.68 +0.4
3,342,645 + Psyr_2754 0.68 -0.9
3,342,645 + Psyr_2754 0.68 +0.2
3,342,646 - Psyr_2754 0.68 -0.8
3,342,646 - Psyr_2754 0.68 -1.6
3,342,646 - Psyr_2754 0.68 -0.6
3,342,652 - Psyr_2754 0.69 -0.1
3,342,652 - Psyr_2754 0.69 +1.2
3,342,690 + Psyr_2754 0.75 +0.5
3,342,690 + Psyr_2754 0.75 -1.5
3,342,691 - Psyr_2754 0.75 +2.4
3,342,691 - Psyr_2754 0.75 -1.3
3,342,691 - Psyr_2754 0.75 +0.5
3,342,691 - Psyr_2754 0.75 +0.7
3,342,691 - Psyr_2754 0.75 +1.6
3,343,167 + Psyr_2755 0.16 -0.6
3,343,283 + Psyr_2755 0.27 +0.2
3,343,284 - Psyr_2755 0.27 +0.5
3,343,284 - Psyr_2755 0.27 +0.5
3,343,286 + Psyr_2755 0.27 +0.9
3,343,287 - Psyr_2755 0.27 -1.3
3,343,287 - Psyr_2755 0.27 -0.7
3,343,287 - Psyr_2755 0.27 +2.0
3,343,691 - Psyr_2755 0.65 +0.4
3,344,103 - +1.0
3,344,103 - -0.2
3,344,105 - +0.2
3,344,106 + +0.7
3,344,107 - -0.6
3,344,107 - -0.8
3,344,107 - +1.3
3,344,110 + +1.0
3,344,110 + +0.4
3,344,110 + +0.4
3,344,111 - -0.1
3,344,114 + -3.1
3,344,115 - +1.0
3,344,116 + +0.1
3,344,117 - -0.4
3,344,184 + -0.1
3,344,185 - -1.8
3,344,185 - +1.2
3,344,374 + Psyr_2756 0.26 -0.2
3,344,374 + Psyr_2756 0.26 -1.4
3,344,374 + Psyr_2756 0.26 -1.5
3,344,374 + Psyr_2756 0.26 -2.9
3,344,374 + Psyr_2756 0.26 -0.2
3,344,374 + Psyr_2756 0.26 -0.6
3,344,375 - Psyr_2756 0.26 +0.4
3,344,375 - Psyr_2756 0.26 -2.1
3,344,377 - Psyr_2756 0.27 +0.4
3,344,481 + Psyr_2756 0.40 +0.3
3,344,481 + Psyr_2756 0.40 -0.9
3,344,481 + Psyr_2756 0.40 +0.9
3,344,482 - Psyr_2756 0.40 +0.2
3,344,486 - Psyr_2756 0.41 +0.0
3,344,528 - Psyr_2756 0.46 -0.5
3,344,528 - Psyr_2756 0.46 -1.0
3,344,528 - Psyr_2756 0.46 +2.6
3,344,549 + Psyr_2756 0.49 -0.9
3,344,567 - Psyr_2756 0.51 -0.9
3,344,755 + Psyr_2756 0.76 -0.2
3,344,756 - Psyr_2756 0.76 -1.0
3,344,938 + +0.4
3,344,939 - -1.4
3,344,939 - +0.0
3,344,939 - +0.6

Or see this region's nucleotide sequence