Experiment: KB with Avocadene 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1763 and Psyr_1764 are separated by 65 nucleotides Psyr_1764 and Psyr_1765 are separated by 26 nucleotides Psyr_1765 and Psyr_1766 are separated by 45 nucleotides Psyr_1766 and Psyr_1767 are separated by 195 nucleotides
Psyr_1763: Psyr_1763 - RNase HI, at 2,004,697 to 2,005,149
_1763
Psyr_1764: Psyr_1764 - DNA polymerase III, epsilon subunit, at 2,005,215 to 2,005,994
_1764
Psyr_1765: Psyr_1765 - GCN5-related N-acetyltransferase, at 2,006,021 to 2,006,569
_1765
Psyr_1766: Psyr_1766 - conserved hypothetical protein, at 2,006,615 to 2,006,830
_1766
Psyr_1767: Psyr_1767 - NADPH-dependent FMN reductase, at 2,007,026 to 2,007,610
_1767
Position (kb)
2006
2007 Strain fitness (log2 ratio)
-2
-1
0
1 at 2005.197 kb on + strand at 2005.197 kb on + strand at 2005.197 kb on + strand at 2006.072 kb on + strand at 2006.072 kb on + strand at 2006.072 kb on + strand at 2006.073 kb on - strand at 2006.073 kb on - strand at 2006.073 kb on - strand at 2006.073 kb on - strand at 2006.099 kb on - strand, within Psyr_1765 at 2006.183 kb on + strand, within Psyr_1765 at 2006.184 kb on - strand, within Psyr_1765 at 2006.276 kb on - strand, within Psyr_1765 at 2006.437 kb on + strand, within Psyr_1765 at 2006.438 kb on - strand, within Psyr_1765 at 2006.459 kb on + strand, within Psyr_1765 at 2006.550 kb on - strand at 2006.616 kb on + strand at 2006.616 kb on + strand at 2006.617 kb on - strand at 2006.617 kb on - strand at 2006.710 kb on - strand, within Psyr_1766 at 2006.710 kb on - strand, within Psyr_1766 at 2006.710 kb on - strand, within Psyr_1766 at 2006.839 kb on + strand at 2006.858 kb on + strand at 2006.858 kb on + strand at 2006.858 kb on + strand at 2006.859 kb on - strand at 2006.859 kb on - strand at 2006.859 kb on - strand at 2006.859 kb on - strand at 2006.871 kb on - strand at 2006.887 kb on + strand at 2006.888 kb on - strand at 2006.890 kb on + strand at 2006.903 kb on + strand at 2006.903 kb on + strand at 2006.903 kb on + strand at 2006.903 kb on + strand at 2006.903 kb on + strand at 2006.904 kb on - strand at 2006.904 kb on - strand at 2006.904 kb on - strand at 2006.905 kb on + strand at 2006.906 kb on - strand at 2006.906 kb on - strand at 2006.984 kb on - strand at 2007.001 kb on + strand at 2007.001 kb on + strand at 2007.001 kb on + strand at 2007.002 kb on - strand at 2007.002 kb on - strand at 2007.002 kb on - strand at 2007.002 kb on - strand at 2007.002 kb on - strand at 2007.002 kb on - strand at 2007.002 kb on - strand at 2007.002 kb on - strand at 2007.003 kb on + strand at 2007.003 kb on + strand at 2007.003 kb on + strand at 2007.004 kb on - strand at 2007.008 kb on - strand at 2007.008 kb on - strand at 2007.055 kb on + strand at 2007.104 kb on - strand, within Psyr_1767 at 2007.182 kb on + strand, within Psyr_1767 at 2007.182 kb on + strand, within Psyr_1767 at 2007.183 kb on - strand, within Psyr_1767 at 2007.183 kb on - strand, within Psyr_1767 at 2007.221 kb on + strand, within Psyr_1767 at 2007.221 kb on + strand, within Psyr_1767 at 2007.221 kb on + strand, within Psyr_1767 at 2007.221 kb on + strand, within Psyr_1767 at 2007.221 kb on + strand, within Psyr_1767 at 2007.221 kb on + strand, within Psyr_1767 at 2007.221 kb on + strand, within Psyr_1767 at 2007.221 kb on + strand, within Psyr_1767 at 2007.221 kb on + strand, within Psyr_1767 at 2007.221 kb on + strand, within Psyr_1767 at 2007.222 kb on - strand, within Psyr_1767 at 2007.222 kb on - strand, within Psyr_1767 at 2007.222 kb on - strand, within Psyr_1767 at 2007.226 kb on + strand, within Psyr_1767 at 2007.281 kb on + strand, within Psyr_1767 at 2007.442 kb on - strand, within Psyr_1767 at 2007.479 kb on + strand, within Psyr_1767 at 2007.480 kb on - strand, within Psyr_1767 at 2007.480 kb on - strand, within Psyr_1767
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Avocadene 10 mM remove 2,005,197 + -0.5 2,005,197 + +0.3 2,005,197 + -0.2 2,006,072 + +0.2 2,006,072 + -1.0 2,006,072 + +0.4 2,006,073 - +0.4 2,006,073 - -1.6 2,006,073 - -0.0 2,006,073 - -0.1 2,006,099 - Psyr_1765 0.14 +1.4 2,006,183 + Psyr_1765 0.30 +1.0 2,006,184 - Psyr_1765 0.30 -1.0 2,006,276 - Psyr_1765 0.46 -1.4 2,006,437 + Psyr_1765 0.76 -2.0 2,006,438 - Psyr_1765 0.76 -0.9 2,006,459 + Psyr_1765 0.80 +1.0 2,006,550 - +0.7 2,006,616 + +0.2 2,006,616 + -0.1 2,006,617 - -0.7 2,006,617 - +0.1 2,006,710 - Psyr_1766 0.44 -1.0 2,006,710 - Psyr_1766 0.44 -0.7 2,006,710 - Psyr_1766 0.44 -0.8 2,006,839 + -0.7 2,006,858 + +1.2 2,006,858 + -1.1 2,006,858 + +1.0 2,006,859 - -0.8 2,006,859 - -1.8 2,006,859 - -0.6 2,006,859 - -0.6 2,006,871 - -0.3 2,006,887 + +1.4 2,006,888 - -2.6 2,006,890 + -1.6 2,006,903 + +0.3 2,006,903 + +1.0 2,006,903 + +0.4 2,006,903 + +0.7 2,006,903 + -0.2 2,006,904 - +0.1 2,006,904 - -0.4 2,006,904 - +0.0 2,006,905 + -0.1 2,006,906 - -0.2 2,006,906 - +0.7 2,006,984 - -0.3 2,007,001 + +0.0 2,007,001 + -0.4 2,007,001 + -0.6 2,007,002 - -1.1 2,007,002 - -0.2 2,007,002 - -0.0 2,007,002 - -0.3 2,007,002 - -2.1 2,007,002 - -0.2 2,007,002 - -0.6 2,007,002 - -2.3 2,007,003 + -0.0 2,007,003 + -0.8 2,007,003 + +1.0 2,007,004 - -0.1 2,007,008 - +0.4 2,007,008 - +0.1 2,007,055 + -1.2 2,007,104 - Psyr_1767 0.13 +0.5 2,007,182 + Psyr_1767 0.27 +0.4 2,007,182 + Psyr_1767 0.27 -0.0 2,007,183 - Psyr_1767 0.27 -0.2 2,007,183 - Psyr_1767 0.27 -2.3 2,007,221 + Psyr_1767 0.33 -0.6 2,007,221 + Psyr_1767 0.33 -2.3 2,007,221 + Psyr_1767 0.33 +0.8 2,007,221 + Psyr_1767 0.33 -0.8 2,007,221 + Psyr_1767 0.33 -1.2 2,007,221 + Psyr_1767 0.33 -0.8 2,007,221 + Psyr_1767 0.33 +0.3 2,007,221 + Psyr_1767 0.33 -0.3 2,007,221 + Psyr_1767 0.33 +0.7 2,007,221 + Psyr_1767 0.33 +0.4 2,007,222 - Psyr_1767 0.34 -1.7 2,007,222 - Psyr_1767 0.34 +0.2 2,007,222 - Psyr_1767 0.34 -0.1 2,007,226 + Psyr_1767 0.34 -2.0 2,007,281 + Psyr_1767 0.44 +0.0 2,007,442 - Psyr_1767 0.71 +0.7 2,007,479 + Psyr_1767 0.77 -1.8 2,007,480 - Psyr_1767 0.78 -0.7 2,007,480 - Psyr_1767 0.78 +0.7
Or see this region's nucleotide sequence