Experiment: KB with Avocadene 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0888 and Psyr_0889 are separated by 147 nucleotides Psyr_0889 and Psyr_0890 overlap by 4 nucleotides Psyr_0890 and Psyr_0891 are separated by 47 nucleotides
Psyr_0888: Psyr_0888 - 3-phosphoshikimate 1-carboxyvinyltransferase, at 1,010,113 to 1,011,369
_0888
Psyr_0889: Psyr_0889 - SpoVT/AbrB-like protein, at 1,011,517 to 1,011,771
_0889
Psyr_0890: Psyr_0890 - PemK-like protein, at 1,011,768 to 1,012,115
_0890
Psyr_0891: Psyr_0891 - anti-ECFsigma factor, ChrR, at 1,012,163 to 1,012,825
_0891
Position (kb)
1011
1012 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1011.371 kb on - strand at 1011.371 kb on - strand at 1011.371 kb on - strand at 1011.371 kb on - strand at 1011.439 kb on + strand at 1011.446 kb on + strand at 1011.447 kb on - strand at 1011.447 kb on - strand at 1011.484 kb on - strand at 1011.484 kb on - strand at 1011.487 kb on + strand at 1011.487 kb on + strand at 1011.487 kb on + strand at 1011.487 kb on + strand at 1011.487 kb on + strand at 1011.487 kb on + strand at 1011.488 kb on - strand at 1011.488 kb on - strand at 1011.488 kb on - strand at 1011.488 kb on - strand at 1011.488 kb on - strand at 1011.488 kb on - strand at 1011.488 kb on - strand at 1011.488 kb on - strand at 1011.492 kb on + strand at 1011.492 kb on + strand at 1011.492 kb on + strand at 1011.493 kb on - strand at 1011.493 kb on - strand at 1011.498 kb on + strand at 1011.498 kb on + strand at 1011.498 kb on + strand at 1011.499 kb on - strand at 1011.503 kb on + strand at 1011.579 kb on - strand, within Psyr_0889 at 1011.579 kb on - strand, within Psyr_0889 at 1011.613 kb on - strand, within Psyr_0889 at 1011.779 kb on + strand at 1011.882 kb on + strand, within Psyr_0890 at 1011.883 kb on - strand, within Psyr_0890 at 1011.883 kb on - strand, within Psyr_0890 at 1011.883 kb on - strand, within Psyr_0890 at 1011.883 kb on - strand, within Psyr_0890 at 1011.901 kb on + strand, within Psyr_0890 at 1011.902 kb on - strand, within Psyr_0890 at 1011.902 kb on - strand, within Psyr_0890 at 1011.902 kb on - strand, within Psyr_0890 at 1011.902 kb on - strand, within Psyr_0890 at 1011.902 kb on - strand, within Psyr_0890 at 1011.902 kb on - strand, within Psyr_0890 at 1011.905 kb on + strand, within Psyr_0890 at 1011.905 kb on + strand, within Psyr_0890 at 1011.905 kb on + strand, within Psyr_0890 at 1011.905 kb on + strand, within Psyr_0890 at 1011.906 kb on - strand, within Psyr_0890 at 1011.906 kb on - strand, within Psyr_0890 at 1011.906 kb on - strand, within Psyr_0890 at 1012.126 kb on + strand at 1012.261 kb on - strand, within Psyr_0891 at 1012.328 kb on + strand, within Psyr_0891 at 1012.328 kb on + strand, within Psyr_0891 at 1012.328 kb on + strand, within Psyr_0891 at 1012.329 kb on - strand, within Psyr_0891 at 1012.329 kb on - strand, within Psyr_0891 at 1012.329 kb on - strand, within Psyr_0891 at 1012.335 kb on + strand, within Psyr_0891 at 1012.336 kb on - strand, within Psyr_0891 at 1012.336 kb on - strand, within Psyr_0891 at 1012.336 kb on - strand, within Psyr_0891 at 1012.464 kb on - strand, within Psyr_0891 at 1012.490 kb on + strand, within Psyr_0891 at 1012.490 kb on + strand, within Psyr_0891 at 1012.491 kb on - strand, within Psyr_0891 at 1012.491 kb on - strand, within Psyr_0891
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Avocadene 10 mM remove 1,011,371 - -0.0 1,011,371 - -0.0 1,011,371 - -0.2 1,011,371 - +2.1 1,011,439 + +0.5 1,011,446 + -0.2 1,011,447 - +0.5 1,011,447 - -0.2 1,011,484 - +0.8 1,011,484 - -0.7 1,011,487 + +1.8 1,011,487 + +0.1 1,011,487 + +0.5 1,011,487 + +1.4 1,011,487 + +0.2 1,011,487 + +0.6 1,011,488 - -0.8 1,011,488 - +0.2 1,011,488 - -0.3 1,011,488 - -1.2 1,011,488 - +1.0 1,011,488 - +0.1 1,011,488 - -0.1 1,011,488 - -0.4 1,011,492 + +0.4 1,011,492 + -0.1 1,011,492 + -1.9 1,011,493 - -0.2 1,011,493 - +0.2 1,011,498 + +0.5 1,011,498 + -0.6 1,011,498 + +0.1 1,011,499 - -0.2 1,011,503 + +1.1 1,011,579 - Psyr_0889 0.24 -1.2 1,011,579 - Psyr_0889 0.24 -2.0 1,011,613 - Psyr_0889 0.38 +1.5 1,011,779 + +0.5 1,011,882 + Psyr_0890 0.33 +2.1 1,011,883 - Psyr_0890 0.33 -0.7 1,011,883 - Psyr_0890 0.33 -0.5 1,011,883 - Psyr_0890 0.33 +0.4 1,011,883 - Psyr_0890 0.33 -2.4 1,011,901 + Psyr_0890 0.38 -0.3 1,011,902 - Psyr_0890 0.39 -0.7 1,011,902 - Psyr_0890 0.39 -0.8 1,011,902 - Psyr_0890 0.39 -1.4 1,011,902 - Psyr_0890 0.39 +1.5 1,011,902 - Psyr_0890 0.39 -0.2 1,011,902 - Psyr_0890 0.39 -1.0 1,011,905 + Psyr_0890 0.39 -1.1 1,011,905 + Psyr_0890 0.39 +0.8 1,011,905 + Psyr_0890 0.39 +0.1 1,011,905 + Psyr_0890 0.39 +0.8 1,011,906 - Psyr_0890 0.40 +0.4 1,011,906 - Psyr_0890 0.40 -0.3 1,011,906 - Psyr_0890 0.40 -0.6 1,012,126 + -0.1 1,012,261 - Psyr_0891 0.15 +0.6 1,012,328 + Psyr_0891 0.25 +0.1 1,012,328 + Psyr_0891 0.25 +0.8 1,012,328 + Psyr_0891 0.25 +0.3 1,012,329 - Psyr_0891 0.25 +0.7 1,012,329 - Psyr_0891 0.25 +0.9 1,012,329 - Psyr_0891 0.25 +0.8 1,012,335 + Psyr_0891 0.26 -0.5 1,012,336 - Psyr_0891 0.26 +0.0 1,012,336 - Psyr_0891 0.26 +0.4 1,012,336 - Psyr_0891 0.26 +0.2 1,012,464 - Psyr_0891 0.45 -0.7 1,012,490 + Psyr_0891 0.49 +0.2 1,012,490 + Psyr_0891 0.49 +0.6 1,012,491 - Psyr_0891 0.49 +0.1 1,012,491 - Psyr_0891 0.49 -0.4
Or see this region's nucleotide sequence