Strain Fitness in Escherichia coli BW25113 around b1386

Experiment: LB Anaerobic with 2-Mercaptopyridine N-oxide 0.000015625 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntfeaB and tynA are separated by 57 nucleotidestynA and maoC are separated by 247 nucleotides b1385: feaB - phenylacetaldehyde dehydrogenase (VIMSS), at 1,445,543 to 1,447,042 feaB b1386: tynA - tyramine oxidase, copper-requiring (NCBI), at 1,447,100 to 1,449,373 tynA b1387: maoC - fused aldehyde dehydrogenase/enoyl-CoA hydratase (NCBI), at 1,449,621 to 1,451,666 maoC Position (kb) 1447 1448 1449 1450Strain fitness (log2 ratio) -2 -1 0 1 2 3at 1446.256 kb on + strand, within feaBat 1446.256 kb on - strand, within feaBat 1446.256 kb on - strand, within feaBat 1446.316 kb on + strand, within feaBat 1446.351 kb on + strand, within feaBat 1446.475 kb on + strand, within feaBat 1446.475 kb on + strand, within feaBat 1446.572 kb on - strand, within feaBat 1446.628 kb on - strand, within feaBat 1446.628 kb on - strand, within feaBat 1446.688 kb on + strand, within feaBat 1446.897 kb on - strandat 1446.946 kb on - strandat 1447.128 kb on - strandat 1447.201 kb on + strandat 1447.254 kb on + strandat 1447.254 kb on + strandat 1447.262 kb on - strandat 1447.278 kb on - strandat 1447.296 kb on - strandat 1447.296 kb on - strandat 1447.365 kb on + strand, within tynAat 1447.365 kb on + strand, within tynAat 1447.594 kb on + strand, within tynAat 1447.887 kb on + strand, within tynAat 1447.996 kb on - strand, within tynAat 1448.027 kb on - strand, within tynAat 1448.027 kb on - strand, within tynAat 1448.229 kb on + strand, within tynAat 1448.229 kb on + strand, within tynAat 1448.318 kb on + strand, within tynAat 1448.378 kb on - strand, within tynAat 1448.378 kb on - strand, within tynAat 1448.583 kb on + strand, within tynAat 1448.640 kb on + strand, within tynAat 1448.730 kb on - strand, within tynAat 1448.730 kb on - strand, within tynAat 1448.757 kb on - strand, within tynAat 1449.263 kb on + strandat 1449.263 kb on - strandat 1449.426 kb on - strandat 1449.443 kb on - strandat 1449.459 kb on + strandat 1449.459 kb on + strandat 1449.468 kb on + strandat 1449.545 kb on - strandat 1449.564 kb on + strandat 1449.685 kb on + strandat 1449.844 kb on - strand, within maoCat 1450.266 kb on - strand, within maoCat 1450.268 kb on + strand, within maoCat 1450.276 kb on - strand, within maoCat 1450.292 kb on + strand, within maoC

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Anaerobic with 2-Mercaptopyridine N-oxide 0.000015625 mM
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1,446,256 + feaB b1385 0.48 +0.1
1,446,256 - feaB b1385 0.48 -0.4
1,446,256 - feaB b1385 0.48 +0.2
1,446,316 + feaB b1385 0.52 +0.3
1,446,351 + feaB b1385 0.54 -0.2
1,446,475 + feaB b1385 0.62 +0.8
1,446,475 + feaB b1385 0.62 -0.7
1,446,572 - feaB b1385 0.69 -0.5
1,446,628 - feaB b1385 0.72 -1.8
1,446,628 - feaB b1385 0.72 -0.5
1,446,688 + feaB b1385 0.76 -1.4
1,446,897 - +1.0
1,446,946 - -1.5
1,447,128 - +0.4
1,447,201 + -0.8
1,447,254 + -1.5
1,447,254 + +0.0
1,447,262 - +0.3
1,447,278 - +0.9
1,447,296 - -0.1
1,447,296 - +1.1
1,447,365 + tynA b1386 0.12 -1.2
1,447,365 + tynA b1386 0.12 +0.8
1,447,594 + tynA b1386 0.22 -0.3
1,447,887 + tynA b1386 0.35 -1.4
1,447,996 - tynA b1386 0.39 -1.8
1,448,027 - tynA b1386 0.41 -0.6
1,448,027 - tynA b1386 0.41 -0.1
1,448,229 + tynA b1386 0.50 +0.1
1,448,229 + tynA b1386 0.50 -1.1
1,448,318 + tynA b1386 0.54 -0.3
1,448,378 - tynA b1386 0.56 +0.0
1,448,378 - tynA b1386 0.56 -1.3
1,448,583 + tynA b1386 0.65 +0.8
1,448,640 + tynA b1386 0.68 +1.7
1,448,730 - tynA b1386 0.72 +0.5
1,448,730 - tynA b1386 0.72 -0.3
1,448,757 - tynA b1386 0.73 -0.1
1,449,263 + -0.7
1,449,263 - -0.6
1,449,426 - +0.8
1,449,443 - +0.0
1,449,459 + -1.7
1,449,459 + +0.6
1,449,468 + -0.6
1,449,545 - +2.8
1,449,564 + +0.9
1,449,685 + -0.4
1,449,844 - maoC b1387 0.11 -0.8
1,450,266 - maoC b1387 0.32 -0.1
1,450,268 + maoC b1387 0.32 +0.9
1,450,276 - maoC b1387 0.32 +0.1
1,450,292 + maoC b1387 0.33 -0.5

Or see this region's nucleotide sequence