Experiment: Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu3450 and Atu3451 are separated by 86 nucleotides Atu3451 and Atu3453 are separated by 424 nucleotides
Atu3450: Atu3450 - nitrilotriacetate monooxygenase, at 498,128 to 499,435
Atu3450
Atu3451: Atu3451 - monooxygenase, at 499,522 to 500,550
Atu3451
Atu3453: Atu3453 - isomerase/lactonizing enzyme, at 500,975 to 502,123
Atu3453
Position (kb) 499
500
501 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 498.572 kb on + strand, within Atu3450 at 498.937 kb on - strand, within Atu3450 at 498.937 kb on - strand, within Atu3450 at 498.991 kb on + strand, within Atu3450 at 499.036 kb on - strand, within Atu3450 at 499.088 kb on + strand, within Atu3450 at 499.154 kb on - strand, within Atu3450 at 499.187 kb on + strand, within Atu3450 at 499.205 kb on - strand, within Atu3450 at 499.205 kb on - strand, within Atu3450 at 499.280 kb on + strand, within Atu3450 at 499.287 kb on + strand, within Atu3450 at 499.287 kb on + strand, within Atu3450 at 499.415 kb on + strand at 499.415 kb on + strand at 499.420 kb on + strand at 499.420 kb on + strand at 499.423 kb on - strand at 499.428 kb on - strand at 499.475 kb on - strand at 499.671 kb on + strand, within Atu3451 at 499.796 kb on - strand, within Atu3451 at 499.806 kb on - strand, within Atu3451 at 500.094 kb on - strand, within Atu3451 at 500.155 kb on + strand, within Atu3451 at 500.187 kb on + strand, within Atu3451 at 500.207 kb on - strand, within Atu3451 at 500.234 kb on + strand, within Atu3451 at 500.248 kb on + strand, within Atu3451 at 500.256 kb on - strand, within Atu3451 at 500.340 kb on + strand, within Atu3451 at 500.404 kb on + strand, within Atu3451 at 500.465 kb on + strand at 500.475 kb on + strand at 500.587 kb on + strand at 500.595 kb on - strand at 500.619 kb on - strand at 500.758 kb on - strand at 500.763 kb on + strand at 500.998 kb on - strand at 501.049 kb on - strand at 501.199 kb on + strand, within Atu3453 at 501.219 kb on + strand, within Atu3453 at 501.233 kb on - strand, within Atu3453 at 501.252 kb on - strand, within Atu3453 at 501.259 kb on + strand, within Atu3453 at 501.289 kb on - strand, within Atu3453 at 501.329 kb on + strand, within Atu3453 at 501.351 kb on - strand, within Atu3453 at 501.373 kb on - strand, within Atu3453 at 501.373 kb on - strand, within Atu3453 at 501.518 kb on + strand, within Atu3453 at 501.532 kb on + strand, within Atu3453
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant remove 498,572 + Atu3450 0.34 -1.4 498,937 - Atu3450 0.62 -1.1 498,937 - Atu3450 0.62 +0.2 498,991 + Atu3450 0.66 +0.2 499,036 - Atu3450 0.69 +0.2 499,088 + Atu3450 0.73 +0.4 499,154 - Atu3450 0.78 +0.5 499,187 + Atu3450 0.81 -0.4 499,205 - Atu3450 0.82 -0.1 499,205 - Atu3450 0.82 +0.0 499,280 + Atu3450 0.88 -0.5 499,287 + Atu3450 0.89 +2.0 499,287 + Atu3450 0.89 -0.1 499,415 + -2.6 499,415 + -0.7 499,420 + -1.4 499,420 + -0.3 499,423 - +0.4 499,428 - -0.6 499,475 - -0.3 499,671 + Atu3451 0.14 +0.2 499,796 - Atu3451 0.27 -0.6 499,806 - Atu3451 0.28 +4.5 500,094 - Atu3451 0.56 +0.0 500,155 + Atu3451 0.62 +1.4 500,187 + Atu3451 0.65 -0.2 500,207 - Atu3451 0.67 -0.9 500,234 + Atu3451 0.69 -0.8 500,248 + Atu3451 0.71 +0.0 500,256 - Atu3451 0.71 -0.9 500,340 + Atu3451 0.79 -2.6 500,404 + Atu3451 0.86 +0.2 500,465 + -1.5 500,475 + +0.2 500,587 + -0.9 500,595 - -0.6 500,619 - +2.3 500,758 - -1.1 500,763 + +1.0 500,998 - -0.6 501,049 - -0.8 501,199 + Atu3453 0.19 +1.1 501,219 + Atu3453 0.21 +2.3 501,233 - Atu3453 0.22 -0.1 501,252 - Atu3453 0.24 +0.0 501,259 + Atu3453 0.25 +0.4 501,289 - Atu3453 0.27 -0.6 501,329 + Atu3453 0.31 -1.8 501,351 - Atu3453 0.33 +0.4 501,373 - Atu3453 0.35 +1.8 501,373 - Atu3453 0.35 -0.3 501,518 + Atu3453 0.47 +0.7 501,532 + Atu3453 0.48 +0.1
Or see this region's nucleotide sequence