Experiment: Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu3399 and Atu3400 are separated by 28 nucleotides Atu3400 and Atu3401 are separated by 189 nucleotides Atu3401 and Atu3402 are separated by 13 nucleotides
Atu3399: Atu3399 - 2-hydroxyacid dehydrogenase, at 443,635 to 444,558
Atu3399
Atu3400: Atu3400 - hypothetical protein, at 444,587 to 444,943
Atu3400
Atu3401: Atu3401 - aldehyde dehydrogenase, at 445,133 to 446,596
Atu3401
Atu3402: Atu3402 - 3-isopropylmalate dehydrogenase, at 446,610 to 447,653
Atu3402
Position (kb) 445
446
447 Strain fitness (log2 ratio) -2
-1
0
1
2 at 444.238 kb on + strand, within Atu3399 at 444.288 kb on - strand, within Atu3399 at 444.288 kb on - strand, within Atu3399 at 444.330 kb on - strand, within Atu3399 at 444.339 kb on + strand, within Atu3399 at 444.347 kb on - strand, within Atu3399 at 444.380 kb on + strand, within Atu3399 at 444.483 kb on - strand at 444.495 kb on + strand at 444.515 kb on + strand at 444.586 kb on - strand at 444.603 kb on - strand at 444.671 kb on + strand, within Atu3400 at 444.685 kb on + strand, within Atu3400 at 444.898 kb on - strand, within Atu3400 at 444.920 kb on + strand at 444.985 kb on - strand at 445.111 kb on - strand at 445.156 kb on - strand at 445.156 kb on - strand at 445.282 kb on + strand, within Atu3401 at 445.342 kb on - strand, within Atu3401 at 445.372 kb on + strand, within Atu3401 at 445.380 kb on - strand, within Atu3401 at 445.385 kb on - strand, within Atu3401 at 445.408 kb on - strand, within Atu3401 at 445.437 kb on - strand, within Atu3401 at 445.437 kb on - strand, within Atu3401 at 445.604 kb on - strand, within Atu3401 at 445.756 kb on + strand, within Atu3401 at 445.756 kb on + strand, within Atu3401 at 445.764 kb on - strand, within Atu3401 at 445.764 kb on - strand, within Atu3401 at 445.795 kb on + strand, within Atu3401 at 445.894 kb on + strand, within Atu3401 at 446.005 kb on + strand, within Atu3401 at 446.033 kb on + strand, within Atu3401 at 446.155 kb on - strand, within Atu3401 at 446.174 kb on + strand, within Atu3401 at 446.212 kb on - strand, within Atu3401 at 446.214 kb on - strand, within Atu3401 at 446.305 kb on - strand, within Atu3401 at 446.359 kb on - strand, within Atu3401 at 446.428 kb on - strand, within Atu3401 at 446.439 kb on - strand, within Atu3401 at 446.449 kb on - strand, within Atu3401 at 446.478 kb on - strand at 446.660 kb on - strand at 446.675 kb on - strand at 446.704 kb on + strand at 446.736 kb on + strand, within Atu3402 at 446.744 kb on - strand, within Atu3402 at 446.749 kb on - strand, within Atu3402 at 446.907 kb on + strand, within Atu3402 at 446.933 kb on + strand, within Atu3402 at 447.056 kb on - strand, within Atu3402 at 447.115 kb on + strand, within Atu3402 at 447.207 kb on + strand, within Atu3402 at 447.243 kb on + strand, within Atu3402 at 447.282 kb on - strand, within Atu3402 at 447.316 kb on + strand, within Atu3402 at 447.316 kb on + strand, within Atu3402 at 447.324 kb on - strand, within Atu3402 at 447.336 kb on + strand, within Atu3402 at 447.336 kb on + strand, within Atu3402 at 447.384 kb on - strand, within Atu3402 at 447.442 kb on + strand, within Atu3402 at 447.442 kb on + strand, within Atu3402 at 447.447 kb on + strand, within Atu3402 at 447.472 kb on + strand, within Atu3402 at 447.495 kb on - strand, within Atu3402
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant remove 444,238 + Atu3399 0.65 -0.7 444,288 - Atu3399 0.71 -1.3 444,288 - Atu3399 0.71 -0.5 444,330 - Atu3399 0.75 -0.2 444,339 + Atu3399 0.76 -0.6 444,347 - Atu3399 0.77 +0.6 444,380 + Atu3399 0.81 -1.0 444,483 - +0.1 444,495 + +2.0 444,515 + +0.1 444,586 - +0.0 444,603 - +0.1 444,671 + Atu3400 0.24 -0.5 444,685 + Atu3400 0.27 +2.2 444,898 - Atu3400 0.87 +0.8 444,920 + +0.2 444,985 - +0.5 445,111 - +0.1 445,156 - -0.0 445,156 - -0.2 445,282 + Atu3401 0.10 -0.1 445,342 - Atu3401 0.14 +0.2 445,372 + Atu3401 0.16 +0.1 445,380 - Atu3401 0.17 -0.0 445,385 - Atu3401 0.17 +1.2 445,408 - Atu3401 0.19 +1.1 445,437 - Atu3401 0.21 +0.3 445,437 - Atu3401 0.21 -1.2 445,604 - Atu3401 0.32 +2.7 445,756 + Atu3401 0.43 -0.2 445,756 + Atu3401 0.43 -1.6 445,764 - Atu3401 0.43 -0.3 445,764 - Atu3401 0.43 -0.6 445,795 + Atu3401 0.45 -0.2 445,894 + Atu3401 0.52 -1.3 446,005 + Atu3401 0.60 +1.1 446,033 + Atu3401 0.61 -0.8 446,155 - Atu3401 0.70 +0.3 446,174 + Atu3401 0.71 -0.3 446,212 - Atu3401 0.74 -0.2 446,214 - Atu3401 0.74 +1.2 446,305 - Atu3401 0.80 -0.6 446,359 - Atu3401 0.84 +1.9 446,428 - Atu3401 0.88 +1.8 446,439 - Atu3401 0.89 +0.7 446,449 - Atu3401 0.90 -0.2 446,478 - -0.1 446,660 - -1.0 446,675 - +0.4 446,704 + -0.3 446,736 + Atu3402 0.12 +0.1 446,744 - Atu3402 0.13 -0.0 446,749 - Atu3402 0.13 -0.5 446,907 + Atu3402 0.28 +0.3 446,933 + Atu3402 0.31 -0.4 447,056 - Atu3402 0.43 +2.6 447,115 + Atu3402 0.48 -0.4 447,207 + Atu3402 0.57 -0.2 447,243 + Atu3402 0.61 -0.7 447,282 - Atu3402 0.64 +0.2 447,316 + Atu3402 0.68 +0.9 447,316 + Atu3402 0.68 -0.4 447,324 - Atu3402 0.68 -0.4 447,336 + Atu3402 0.70 -1.8 447,336 + Atu3402 0.70 -1.0 447,384 - Atu3402 0.74 -1.0 447,442 + Atu3402 0.80 -0.5 447,442 + Atu3402 0.80 -0.2 447,447 + Atu3402 0.80 -0.1 447,472 + Atu3402 0.83 +0.8 447,495 - Atu3402 0.85 -2.1
Or see this region's nucleotide sequence