Strain Fitness in Escherichia coli BW25113 around b2781

Experiment: LB Anaerobic with Irgasan 0.000000625 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntpyrG and mazG are separated by 227 nucleotidesmazG and chpA are separated by 70 nucleotideschpA and chpR overlap by 1 nucleotideschpR and relA are separated by 77 nucleotides b2780: pyrG - CTP synthetase (NCBI), at 2,906,051 to 2,907,688 pyrG b2781: mazG - nucleoside triphosphate pyrophosphohydrolase (NCBI), at 2,907,916 to 2,908,707 mazG b2782: chpA - toxin of the ChpA-ChpR toxin-antitoxin system, endoribonuclease (NCBI), at 2,908,778 to 2,909,113 chpA b2783: chpR - antitoxin of the ChpA-ChpR toxin-antitoxin system (NCBI), at 2,909,113 to 2,909,361 chpR b2784: relA - (p)ppGpp synthetase I/GTP pyrophosphokinase (NCBI), at 2,909,439 to 2,911,673 relA Position (kb) 2907 2908 2909Strain fitness (log2 ratio) -3 -2 -1 0 1at 2907.991 kb on - strandat 2908.016 kb on - strand, within mazGat 2908.022 kb on - strand, within mazGat 2908.082 kb on + strand, within mazGat 2908.122 kb on - strand, within mazGat 2908.157 kb on - strand, within mazGat 2908.172 kb on + strand, within mazGat 2908.172 kb on + strand, within mazGat 2908.377 kb on - strand, within mazGat 2908.538 kb on + strand, within mazGat 2908.571 kb on + strand, within mazGat 2908.624 kb on + strand, within mazGat 2908.674 kb on + strandat 2908.674 kb on + strandat 2908.674 kb on + strandat 2908.674 kb on + strandat 2908.682 kb on - strandat 2908.754 kb on - strandat 2908.754 kb on - strandat 2908.764 kb on - strandat 2908.764 kb on - strandat 2908.772 kb on + strandat 2908.772 kb on + strandat 2908.854 kb on + strand, within chpAat 2908.991 kb on - strand, within chpAat 2909.017 kb on - strand, within chpAat 2909.017 kb on - strand, within chpAat 2909.032 kb on + strand, within chpAat 2909.032 kb on + strand, within chpAat 2909.253 kb on + strand, within chpRat 2909.258 kb on + strand, within chpRat 2909.311 kb on + strand, within chpRat 2909.487 kb on + strandat 2909.487 kb on + strandat 2909.490 kb on - strandat 2909.490 kb on - strandat 2909.556 kb on - strandat 2909.669 kb on - strand, within relA

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Anaerobic with Irgasan 0.000000625 mM
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2,907,991 - +0.0
2,908,016 - mazG b2781 0.13 -0.6
2,908,022 - mazG b2781 0.13 -1.3
2,908,082 + mazG b2781 0.21 +0.1
2,908,122 - mazG b2781 0.26 -0.5
2,908,157 - mazG b2781 0.30 -1.9
2,908,172 + mazG b2781 0.32 +0.1
2,908,172 + mazG b2781 0.32 +0.1
2,908,377 - mazG b2781 0.58 -0.0
2,908,538 + mazG b2781 0.79 -1.4
2,908,571 + mazG b2781 0.83 -0.4
2,908,624 + mazG b2781 0.89 -1.6
2,908,674 + -0.3
2,908,674 + +0.3
2,908,674 + -0.0
2,908,674 + +0.0
2,908,682 - -1.6
2,908,754 - -0.2
2,908,754 - +0.0
2,908,764 - -1.8
2,908,764 - +0.2
2,908,772 + +0.1
2,908,772 + -2.0
2,908,854 + chpA b2782 0.23 +0.0
2,908,991 - chpA b2782 0.63 +0.6
2,909,017 - chpA b2782 0.71 +0.3
2,909,017 - chpA b2782 0.71 +0.0
2,909,032 + chpA b2782 0.76 -0.9
2,909,032 + chpA b2782 0.76 +0.4
2,909,253 + chpR b2783 0.56 -1.2
2,909,258 + chpR b2783 0.58 -0.9
2,909,311 + chpR b2783 0.80 -3.5
2,909,487 + -2.2
2,909,487 + -2.2
2,909,490 - +0.3
2,909,490 - +0.4
2,909,556 - -0.6
2,909,669 - relA b2784 0.10 -2.0

Or see this region's nucleotide sequence