Strain Fitness in Escherichia coli BW25113 around b2205

Experiment: LB Anaerobic with Irgasan 0.000000625 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntnapB and napH overlap by 4 nucleotidesnapH and napG overlap by 14 nucleotidesnapG and napA are separated by 6 nucleotides b2203: napB - cytochrome c-type protein (VIMSS), at 2,296,291 to 2,296,740 napB b2204: napH - quinol dehydrogenase membrane component (NCBI), at 2,296,737 to 2,297,600 napH b2205: napG - quinol dehydrogenase periplasmic component (NCBI), at 2,297,587 to 2,298,282 napG b2206: napA - nitrate reductase, periplasmic, large subunit (NCBI), at 2,298,289 to 2,300,775 napA Position (kb) 2297 2298 2299Strain fitness (log2 ratio) -2 -1 0 1 2at 2296.675 kb on + strand, within napBat 2296.695 kb on - strand, within napBat 2296.773 kb on + strandat 2296.773 kb on + strandat 2296.779 kb on - strandat 2296.880 kb on - strand, within napHat 2296.953 kb on + strand, within napHat 2297.095 kb on - strand, within napHat 2297.341 kb on + strand, within napHat 2297.444 kb on - strand, within napHat 2297.464 kb on + strand, within napHat 2297.476 kb on - strand, within napHat 2297.476 kb on - strand, within napHat 2297.517 kb on + strandat 2297.535 kb on - strandat 2297.535 kb on - strandat 2297.585 kb on - strandat 2297.585 kb on - strandat 2297.615 kb on - strandat 2297.676 kb on - strand, within napGat 2297.791 kb on + strand, within napGat 2297.798 kb on + strand, within napGat 2297.949 kb on + strand, within napGat 2297.953 kb on - strand, within napGat 2298.068 kb on + strand, within napGat 2298.073 kb on + strand, within napGat 2298.161 kb on - strand, within napGat 2298.190 kb on + strand, within napGat 2298.194 kb on + strand, within napGat 2298.340 kb on - strandat 2298.340 kb on - strandat 2298.469 kb on + strandat 2298.562 kb on + strand, within napAat 2298.565 kb on + strand, within napAat 2298.570 kb on - strand, within napAat 2298.572 kb on + strand, within napAat 2298.578 kb on - strand, within napAat 2298.582 kb on + strand, within napAat 2298.582 kb on + strand, within napAat 2298.604 kb on - strand, within napAat 2298.604 kb on - strand, within napAat 2298.790 kb on + strand, within napAat 2298.882 kb on - strand, within napAat 2298.981 kb on + strand, within napAat 2298.981 kb on + strand, within napAat 2298.983 kb on + strand, within napAat 2298.983 kb on + strand, within napAat 2299.128 kb on + strand, within napAat 2299.128 kb on + strand, within napAat 2299.139 kb on + strand, within napAat 2299.195 kb on + strand, within napAat 2299.195 kb on + strand, within napAat 2299.267 kb on - strand, within napA

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Anaerobic with Irgasan 0.000000625 mM
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2,296,675 + napB b2203 0.85 +0.8
2,296,695 - napB b2203 0.90 -1.1
2,296,773 + -1.1
2,296,773 + +0.9
2,296,779 - -0.8
2,296,880 - napH b2204 0.17 +0.4
2,296,953 + napH b2204 0.25 -1.8
2,297,095 - napH b2204 0.41 -0.3
2,297,341 + napH b2204 0.70 -0.1
2,297,444 - napH b2204 0.82 +0.9
2,297,464 + napH b2204 0.84 +0.7
2,297,476 - napH b2204 0.86 +2.3
2,297,476 - napH b2204 0.86 +0.5
2,297,517 + +1.3
2,297,535 - -0.1
2,297,535 - -1.3
2,297,585 - -0.2
2,297,585 - -1.6
2,297,615 - +0.7
2,297,676 - napG b2205 0.13 -0.2
2,297,791 + napG b2205 0.29 -0.8
2,297,798 + napG b2205 0.30 -0.2
2,297,949 + napG b2205 0.52 +0.6
2,297,953 - napG b2205 0.53 -0.0
2,298,068 + napG b2205 0.69 -0.0
2,298,073 + napG b2205 0.70 -0.1
2,298,161 - napG b2205 0.82 -0.0
2,298,190 + napG b2205 0.87 -0.5
2,298,194 + napG b2205 0.87 -1.8
2,298,340 - +0.1
2,298,340 - +0.8
2,298,469 + +0.4
2,298,562 + napA b2206 0.11 -0.9
2,298,565 + napA b2206 0.11 +0.7
2,298,570 - napA b2206 0.11 +0.5
2,298,572 + napA b2206 0.11 +0.3
2,298,578 - napA b2206 0.12 +0.1
2,298,582 + napA b2206 0.12 -0.7
2,298,582 + napA b2206 0.12 -0.9
2,298,604 - napA b2206 0.13 +0.3
2,298,604 - napA b2206 0.13 +0.3
2,298,790 + napA b2206 0.20 +0.1
2,298,882 - napA b2206 0.24 -0.4
2,298,981 + napA b2206 0.28 +0.8
2,298,981 + napA b2206 0.28 +0.2
2,298,983 + napA b2206 0.28 +0.4
2,298,983 + napA b2206 0.28 +0.0
2,299,128 + napA b2206 0.34 +0.3
2,299,128 + napA b2206 0.34 +0.8
2,299,139 + napA b2206 0.34 +0.4
2,299,195 + napA b2206 0.36 +0.6
2,299,195 + napA b2206 0.36 -0.8
2,299,267 - napA b2206 0.39 -0.0

Or see this region's nucleotide sequence