Strain Fitness in Escherichia coli BW25113 around b0954

Experiment: LB Anaerobic with Irgasan 0.000000625 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntymbA and rmf are separated by 255 nucleotidesrmf and fabA are separated by 69 nucleotidesfabA and ycbZ are separated by 68 nucleotides b0952: ymbA - conserved protein (RefSeq), at 1,014,119 to 1,014,682 ymbA b0953: rmf - ribosome modulation factor (NCBI), at 1,014,938 to 1,015,105 rmf b0954: fabA - 3-hydroxydecanoyl-ACP dehydratase (NCBI), at 1,015,175 to 1,015,693 fabA b0955: ycbZ - predicted peptidase (NCBI), at 1,015,762 to 1,017,522 ycbZ Position (kb) 1015 1016Strain fitness (log2 ratio) -2 -1 0 1at 1014.206 kb on + strand, within ymbAat 1014.216 kb on + strand, within ymbAat 1014.216 kb on + strand, within ymbAat 1014.263 kb on + strand, within ymbAat 1014.268 kb on + strand, within ymbAat 1014.303 kb on - strand, within ymbAat 1014.428 kb on - strand, within ymbAat 1014.428 kb on - strand, within ymbAat 1014.587 kb on + strand, within ymbAat 1014.588 kb on + strand, within ymbAat 1014.588 kb on + strand, within ymbAat 1014.619 kb on - strand, within ymbAat 1014.959 kb on + strand, within rmfat 1014.974 kb on + strand, within rmfat 1014.974 kb on + strand, within rmfat 1015.089 kb on + strandat 1015.097 kb on - strandat 1015.736 kb on - strandat 1015.736 kb on - strandat 1015.736 kb on - strandat 1015.740 kb on - strandat 1015.740 kb on - strandat 1015.876 kb on + strandat 1015.878 kb on - strandat 1015.894 kb on - strandat 1015.922 kb on - strandat 1015.935 kb on + strandat 1015.967 kb on - strand, within ycbZat 1015.967 kb on - strand, within ycbZat 1015.989 kb on + strand, within ycbZat 1016.003 kb on + strand, within ycbZat 1016.003 kb on + strand, within ycbZat 1016.012 kb on + strand, within ycbZat 1016.191 kb on + strand, within ycbZat 1016.228 kb on + strand, within ycbZat 1016.236 kb on + strand, within ycbZat 1016.667 kb on + strand, within ycbZat 1016.667 kb on + strand, within ycbZ

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Anaerobic with Irgasan 0.000000625 mM
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1,014,206 + ymbA b0952 0.15 -1.0
1,014,216 + ymbA b0952 0.17 +0.0
1,014,216 + ymbA b0952 0.17 +0.9
1,014,263 + ymbA b0952 0.26 -0.7
1,014,268 + ymbA b0952 0.26 -0.3
1,014,303 - ymbA b0952 0.33 +0.1
1,014,428 - ymbA b0952 0.55 +1.5
1,014,428 - ymbA b0952 0.55 -1.2
1,014,587 + ymbA b0952 0.83 -0.3
1,014,588 + ymbA b0952 0.83 +0.2
1,014,588 + ymbA b0952 0.83 -0.6
1,014,619 - ymbA b0952 0.89 -2.0
1,014,959 + rmf b0953 0.12 +0.4
1,014,974 + rmf b0953 0.21 -1.5
1,014,974 + rmf b0953 0.21 -1.0
1,015,089 + +1.1
1,015,097 - +0.2
1,015,736 - +0.2
1,015,736 - +0.4
1,015,736 - -0.4
1,015,740 - -1.5
1,015,740 - -0.1
1,015,876 + -0.1
1,015,878 - -0.7
1,015,894 - -1.8
1,015,922 - -0.1
1,015,935 + +1.0
1,015,967 - ycbZ b0955 0.12 -0.4
1,015,967 - ycbZ b0955 0.12 -1.1
1,015,989 + ycbZ b0955 0.13 -1.5
1,016,003 + ycbZ b0955 0.14 -0.7
1,016,003 + ycbZ b0955 0.14 -0.3
1,016,012 + ycbZ b0955 0.14 +0.4
1,016,191 + ycbZ b0955 0.24 +1.7
1,016,228 + ycbZ b0955 0.26 -0.4
1,016,236 + ycbZ b0955 0.27 -1.0
1,016,667 + ycbZ b0955 0.51 -0.8
1,016,667 + ycbZ b0955 0.51 -1.5

Or see this region's nucleotide sequence