Strain Fitness in Escherichia coli BW25113 around b4217

Experiment: LB Anaerobic with Nitrofurantoin 0.000015625 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntytfI and ytfJ are separated by 62 nucleotidesytfJ and ytfK are separated by 324 nucleotidesytfK and ytfL are separated by 78 nucleotides b4215: ytfI - hypothetical protein (NCBI), at 4,435,730 to 4,436,668 ytfI b4216: ytfJ - predicted transcriptional regulator (NCBI), at 4,436,731 to 4,437,285 ytfJ b4217: ytfK - orf, hypothetical protein (VIMSS), at 4,437,610 to 4,437,816 ytfK b4218: ytfL - predicted inner membrane protein (NCBI), at 4,437,895 to 4,439,238 ytfL Position (kb) 4437 4438Strain fitness (log2 ratio) -2 -1 0 1 2at 4436.636 kb on + strandat 4436.679 kb on - strandat 4436.679 kb on - strandat 4436.686 kb on - strandat 4436.692 kb on - strandat 4436.692 kb on - strandat 4436.695 kb on - strandat 4436.762 kb on - strandat 4436.779 kb on - strandat 4436.781 kb on - strandat 4436.781 kb on - strandat 4436.783 kb on - strandat 4436.783 kb on - strandat 4436.783 kb on - strandat 4436.783 kb on - strandat 4436.795 kb on + strand, within ytfJat 4436.813 kb on - strand, within ytfJat 4436.814 kb on - strand, within ytfJat 4436.816 kb on - strand, within ytfJat 4436.816 kb on - strand, within ytfJat 4436.819 kb on + strand, within ytfJat 4436.819 kb on + strand, within ytfJat 4436.821 kb on - strand, within ytfJat 4436.827 kb on - strand, within ytfJat 4436.827 kb on - strand, within ytfJat 4436.827 kb on - strand, within ytfJat 4436.827 kb on - strand, within ytfJat 4436.827 kb on - strand, within ytfJat 4436.838 kb on - strand, within ytfJat 4436.838 kb on - strand, within ytfJat 4436.870 kb on - strand, within ytfJat 4436.870 kb on - strand, within ytfJat 4436.870 kb on - strand, within ytfJat 4436.902 kb on - strand, within ytfJat 4436.902 kb on - strand, within ytfJat 4436.929 kb on + strand, within ytfJat 4436.991 kb on + strand, within ytfJat 4437.003 kb on + strand, within ytfJat 4437.203 kb on - strand, within ytfJat 4437.233 kb on + strandat 4437.239 kb on + strandat 4437.247 kb on + strandat 4437.251 kb on + strandat 4437.281 kb on + strandat 4437.281 kb on + strandat 4437.282 kb on - strandat 4437.282 kb on - strandat 4437.287 kb on + strandat 4437.287 kb on - strandat 4437.289 kb on - strandat 4437.289 kb on - strandat 4437.289 kb on - strandat 4437.300 kb on - strandat 4437.316 kb on - strandat 4437.323 kb on + strandat 4437.363 kb on - strandat 4437.363 kb on - strandat 4437.395 kb on + strandat 4437.420 kb on + strandat 4437.420 kb on + strandat 4437.425 kb on + strandat 4437.519 kb on + strandat 4437.526 kb on - strandat 4437.526 kb on - strandat 4437.527 kb on - strandat 4437.527 kb on - strandat 4437.574 kb on + strandat 4437.635 kb on + strand, within ytfKat 4437.635 kb on + strand, within ytfKat 4437.660 kb on - strand, within ytfKat 4437.686 kb on - strand, within ytfKat 4437.686 kb on - strand, within ytfKat 4437.692 kb on - strand, within ytfKat 4437.717 kb on + strand, within ytfKat 4437.768 kb on + strand, within ytfKat 4437.768 kb on + strand, within ytfKat 4437.786 kb on + strand, within ytfKat 4437.788 kb on - strand, within ytfKat 4437.858 kb on - strandat 4437.858 kb on - strandat 4437.860 kb on + strandat 4437.860 kb on + strandat 4437.946 kb on + strandat 4437.958 kb on + strandat 4437.976 kb on - strandat 4437.979 kb on + strandat 4437.997 kb on + strandat 4437.997 kb on + strandat 4438.030 kb on + strand, within ytfLat 4438.071 kb on + strand, within ytfLat 4438.072 kb on - strand, within ytfLat 4438.081 kb on - strand, within ytfLat 4438.081 kb on - strand, within ytfLat 4438.226 kb on - strand, within ytfLat 4438.226 kb on - strand, within ytfLat 4438.281 kb on + strand, within ytfLat 4438.355 kb on - strand, within ytfLat 4438.355 kb on - strand, within ytfLat 4438.369 kb on - strand, within ytfLat 4438.390 kb on + strand, within ytfLat 4438.390 kb on + strand, within ytfLat 4438.408 kb on - strand, within ytfLat 4438.408 kb on - strand, within ytfLat 4438.431 kb on + strand, within ytfLat 4438.438 kb on + strand, within ytfLat 4438.444 kb on - strand, within ytfLat 4438.458 kb on + strand, within ytfLat 4438.458 kb on + strand, within ytfLat 4438.474 kb on - strand, within ytfLat 4438.510 kb on + strand, within ytfLat 4438.527 kb on + strand, within ytfLat 4438.568 kb on - strand, within ytfLat 4438.595 kb on - strand, within ytfLat 4438.595 kb on - strand, within ytfLat 4438.694 kb on - strand, within ytfLat 4438.702 kb on - strand, within ytfLat 4438.731 kb on + strand, within ytfLat 4438.739 kb on - strand, within ytfLat 4438.739 kb on - strand, within ytfLat 4438.755 kb on + strand, within ytfLat 4438.755 kb on + strand, within ytfLat 4438.762 kb on - strand, within ytfLat 4438.762 kb on - strand, within ytfLat 4438.775 kb on + strand, within ytfLat 4438.775 kb on + strand, within ytfL

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Anaerobic with Nitrofurantoin 0.000015625 mM
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4,436,636 + +1.1
4,436,679 - -1.5
4,436,679 - +0.1
4,436,686 - -0.5
4,436,692 - -1.2
4,436,692 - +1.1
4,436,695 - -0.2
4,436,762 - +0.5
4,436,779 - -1.9
4,436,781 - +0.9
4,436,781 - +0.0
4,436,783 - -0.0
4,436,783 - +0.1
4,436,783 - +0.1
4,436,783 - -1.0
4,436,795 + ytfJ b4216 0.12 +0.1
4,436,813 - ytfJ b4216 0.15 +0.3
4,436,814 - ytfJ b4216 0.15 +0.1
4,436,816 - ytfJ b4216 0.15 +0.4
4,436,816 - ytfJ b4216 0.15 -0.8
4,436,819 + ytfJ b4216 0.16 +0.1
4,436,819 + ytfJ b4216 0.16 +0.7
4,436,821 - ytfJ b4216 0.16 +0.4
4,436,827 - ytfJ b4216 0.17 -0.5
4,436,827 - ytfJ b4216 0.17 +0.0
4,436,827 - ytfJ b4216 0.17 +0.4
4,436,827 - ytfJ b4216 0.17 -0.1
4,436,827 - ytfJ b4216 0.17 -1.0
4,436,838 - ytfJ b4216 0.19 -0.6
4,436,838 - ytfJ b4216 0.19 -0.5
4,436,870 - ytfJ b4216 0.25 +0.1
4,436,870 - ytfJ b4216 0.25 +0.3
4,436,870 - ytfJ b4216 0.25 -0.2
4,436,902 - ytfJ b4216 0.31 +0.5
4,436,902 - ytfJ b4216 0.31 -1.5
4,436,929 + ytfJ b4216 0.36 -1.5
4,436,991 + ytfJ b4216 0.47 +1.1
4,437,003 + ytfJ b4216 0.49 +0.1
4,437,203 - ytfJ b4216 0.85 -0.0
4,437,233 + +0.2
4,437,239 + +0.2
4,437,247 + -0.6
4,437,251 + -0.9
4,437,281 + +0.1
4,437,281 + +1.1
4,437,282 - -0.3
4,437,282 - -0.3
4,437,287 + -0.9
4,437,287 - -0.5
4,437,289 - -0.4
4,437,289 - -0.3
4,437,289 - -1.0
4,437,300 - +0.4
4,437,316 - -0.7
4,437,323 + -0.3
4,437,363 - +0.5
4,437,363 - +0.2
4,437,395 + +0.5
4,437,420 + -0.5
4,437,420 + +0.2
4,437,425 + +1.1
4,437,519 + -1.5
4,437,526 - -1.3
4,437,526 - -0.8
4,437,527 - +0.2
4,437,527 - -0.1
4,437,574 + -0.3
4,437,635 + ytfK b4217 0.12 +0.2
4,437,635 + ytfK b4217 0.12 +0.3
4,437,660 - ytfK b4217 0.24 -0.2
4,437,686 - ytfK b4217 0.37 +0.5
4,437,686 - ytfK b4217 0.37 -1.5
4,437,692 - ytfK b4217 0.40 +0.2
4,437,717 + ytfK b4217 0.52 +0.1
4,437,768 + ytfK b4217 0.76 +0.4
4,437,768 + ytfK b4217 0.76 +1.2
4,437,786 + ytfK b4217 0.85 +0.4
4,437,788 - ytfK b4217 0.86 -0.3
4,437,858 - +0.2
4,437,858 - -0.8
4,437,860 + +0.1
4,437,860 + -0.4
4,437,946 + +0.3
4,437,958 + +0.9
4,437,976 - +0.5
4,437,979 + -0.9
4,437,997 + -0.2
4,437,997 + -0.1
4,438,030 + ytfL b4218 0.10 -0.3
4,438,071 + ytfL b4218 0.13 -0.3
4,438,072 - ytfL b4218 0.13 +0.1
4,438,081 - ytfL b4218 0.14 -0.5
4,438,081 - ytfL b4218 0.14 +1.1
4,438,226 - ytfL b4218 0.25 -0.7
4,438,226 - ytfL b4218 0.25 -0.4
4,438,281 + ytfL b4218 0.29 +0.7
4,438,355 - ytfL b4218 0.34 +0.2
4,438,355 - ytfL b4218 0.34 -0.4
4,438,369 - ytfL b4218 0.35 -0.3
4,438,390 + ytfL b4218 0.37 -1.1
4,438,390 + ytfL b4218 0.37 -0.6
4,438,408 - ytfL b4218 0.38 -0.6
4,438,408 - ytfL b4218 0.38 +0.1
4,438,431 + ytfL b4218 0.40 -0.5
4,438,438 + ytfL b4218 0.40 +1.1
4,438,444 - ytfL b4218 0.41 -1.0
4,438,458 + ytfL b4218 0.42 -0.3
4,438,458 + ytfL b4218 0.42 -0.5
4,438,474 - ytfL b4218 0.43 -0.2
4,438,510 + ytfL b4218 0.46 -0.7
4,438,527 + ytfL b4218 0.47 -1.2
4,438,568 - ytfL b4218 0.50 +0.4
4,438,595 - ytfL b4218 0.52 +0.4
4,438,595 - ytfL b4218 0.52 -0.5
4,438,694 - ytfL b4218 0.59 -1.1
4,438,702 - ytfL b4218 0.60 +0.4
4,438,731 + ytfL b4218 0.62 -0.6
4,438,739 - ytfL b4218 0.63 +0.9
4,438,739 - ytfL b4218 0.63 -0.2
4,438,755 + ytfL b4218 0.64 -0.6
4,438,755 + ytfL b4218 0.64 +2.4
4,438,762 - ytfL b4218 0.65 -0.1
4,438,762 - ytfL b4218 0.65 +0.6
4,438,775 + ytfL b4218 0.65 -0.6
4,438,775 + ytfL b4218 0.65 -1.4

Or see this region's nucleotide sequence