Experiment: LB Anaerobic with Nitrofurantoin 0.000015625 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt modF and modE are separated by 67 nucleotides modE and ybhT are separated by 128 nucleotides ybhT and modA are separated by 166 nucleotides
b0760: modF - fused molybdate transporter subunits of ABC superfamily: ATP-binding components (NCBI), at 791,539 to 793,011
modF
b0761: modE - DNA-binding transcriptional dual regulator (NCBI), at 793,079 to 793,867
modE
b0762: ybhT - hypothetical protein (NCBI), at 793,996 to 794,145
ybhT
b0763: modA - molybdate transporter subunit (NCBI), at 794,312 to 795,085
modA
Position (kb) 793
794 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 792.228 kb on - strand, within modF at 792.450 kb on + strand, within modF at 792.452 kb on - strand, within modF at 792.452 kb on - strand, within modF at 792.473 kb on - strand, within modF at 792.473 kb on - strand, within modF at 792.607 kb on - strand, within modF at 792.607 kb on - strand, within modF at 792.687 kb on - strand, within modF at 792.742 kb on - strand, within modF at 792.744 kb on + strand, within modF at 792.755 kb on + strand, within modF at 792.757 kb on - strand, within modF at 792.757 kb on - strand, within modF at 792.882 kb on - strand at 792.882 kb on - strand at 793.046 kb on + strand at 793.085 kb on - strand at 793.085 kb on - strand at 793.183 kb on + strand, within modE at 793.183 kb on + strand, within modE at 793.191 kb on - strand, within modE at 793.212 kb on + strand, within modE at 793.212 kb on + strand, within modE at 793.213 kb on - strand, within modE at 793.217 kb on + strand, within modE at 793.230 kb on - strand, within modE at 793.230 kb on - strand, within modE at 793.300 kb on - strand, within modE at 793.310 kb on - strand, within modE at 793.538 kb on - strand, within modE at 793.538 kb on - strand, within modE at 793.629 kb on - strand, within modE at 793.629 kb on - strand, within modE at 793.631 kb on + strand, within modE at 793.631 kb on + strand, within modE at 793.636 kb on + strand, within modE at 793.639 kb on - strand, within modE at 793.639 kb on - strand, within modE at 793.639 kb on - strand, within modE at 793.683 kb on - strand, within modE at 793.683 kb on - strand, within modE at 793.685 kb on + strand, within modE at 793.735 kb on + strand, within modE at 793.735 kb on + strand, within modE at 793.837 kb on + strand at 793.847 kb on - strand at 793.864 kb on + strand at 793.864 kb on + strand at 793.872 kb on - strand at 793.872 kb on - strand at 793.942 kb on + strand at 793.942 kb on + strand at 794.427 kb on + strand, within modA at 794.450 kb on - strand, within modA at 794.521 kb on - strand, within modA at 794.527 kb on + strand, within modA at 794.606 kb on + strand, within modA at 794.606 kb on + strand, within modA at 794.628 kb on + strand, within modA at 794.800 kb on + strand, within modA
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Nitrofurantoin 0.000015625 mM remove 792,228 - modF b0760 0.47 +0.1 792,450 + modF b0760 0.62 -1.2 792,452 - modF b0760 0.62 +0.6 792,452 - modF b0760 0.62 +0.1 792,473 - modF b0760 0.63 +1.4 792,473 - modF b0760 0.63 +0.2 792,607 - modF b0760 0.73 -1.0 792,607 - modF b0760 0.73 -0.1 792,687 - modF b0760 0.78 +1.4 792,742 - modF b0760 0.82 -0.2 792,744 + modF b0760 0.82 +0.5 792,755 + modF b0760 0.83 -1.1 792,757 - modF b0760 0.83 -0.7 792,757 - modF b0760 0.83 -0.2 792,882 - -2.3 792,882 - -0.6 793,046 + -0.5 793,085 - +0.8 793,085 - +3.2 793,183 + modE b0761 0.13 -0.5 793,183 + modE b0761 0.13 -0.7 793,191 - modE b0761 0.14 -2.3 793,212 + modE b0761 0.17 -0.7 793,212 + modE b0761 0.17 +0.8 793,213 - modE b0761 0.17 -1.1 793,217 + modE b0761 0.17 -0.2 793,230 - modE b0761 0.19 -0.9 793,230 - modE b0761 0.19 -2.2 793,300 - modE b0761 0.28 -0.3 793,310 - modE b0761 0.29 -0.2 793,538 - modE b0761 0.58 +0.8 793,538 - modE b0761 0.58 -0.2 793,629 - modE b0761 0.70 -0.7 793,629 - modE b0761 0.70 -0.3 793,631 + modE b0761 0.70 +0.1 793,631 + modE b0761 0.70 -0.5 793,636 + modE b0761 0.71 +0.0 793,639 - modE b0761 0.71 -2.3 793,639 - modE b0761 0.71 -0.4 793,639 - modE b0761 0.71 -0.3 793,683 - modE b0761 0.77 -1.2 793,683 - modE b0761 0.77 -0.7 793,685 + modE b0761 0.77 +0.3 793,735 + modE b0761 0.83 -2.5 793,735 + modE b0761 0.83 -1.7 793,837 + -2.0 793,847 - -1.3 793,864 + -2.2 793,864 + -0.5 793,872 - -1.4 793,872 - +0.4 793,942 + +2.8 793,942 + +0.1 794,427 + modA b0763 0.15 -2.3 794,450 - modA b0763 0.18 -3.0 794,521 - modA b0763 0.27 -1.5 794,527 + modA b0763 0.28 -1.6 794,606 + modA b0763 0.38 -2.9 794,606 + modA b0763 0.38 -0.9 794,628 + modA b0763 0.41 -0.6 794,800 + modA b0763 0.63 -1.6
Or see this region's nucleotide sequence