Strain Fitness in Escherichia coli BW25113 around b4464

Experiment: LB Anaerobic with Nitrofurantoin 0.00003125 mM

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	LB Anaerobic with Nitrofurantoin 0.00003125 mM
3,024,199	+	guaD	b2883	0.31	+0.9
3,024,207	+	guaD	b2883	0.32	-2.8
3,024,217	+	guaD	b2883	0.33	+0.2
3,024,251	-	guaD	b2883	0.35	+1.4
3,024,251	-	guaD	b2883	0.35	-0.3
3,024,310	-	guaD	b2883	0.40	-1.2
3,024,310	-	guaD	b2883	0.40	+0.4
3,024,321	+	guaD	b2883	0.40	-1.0
3,024,321	+	guaD	b2883	0.40	+0.4
3,024,321	+	guaD	b2883	0.40	+0.1
3,024,418	+	guaD	b2883	0.48	+1.3
3,024,482	+	guaD	b2883	0.53	+0.0
3,024,490	-	guaD	b2883	0.53	+0.5
3,024,615	-	guaD	b2883	0.63	-0.3
3,024,668	+	guaD	b2883	0.67	+1.8
3,024,668	+	guaD	b2883	0.67	+1.8
3,024,676	-	guaD	b2883	0.67	-0.8
3,025,060	+				+0.9
3,025,134	-				-2.3
3,025,138	+				+0.7
3,025,138	+				+0.4
3,025,502	+	ygfQ	b4464	0.26	-1.1
3,025,587	-	ygfQ	b4464	0.32	-0.8
3,025,589	+	ygfQ	b4464	0.33	-0.1
3,025,591	+	ygfQ	b4464	0.33	-1.8
3,025,599	-	ygfQ	b4464	0.33	+0.4
3,025,643	+	ygfQ	b4464	0.37	-1.1
3,025,755	-	ygfQ	b4464	0.45	-1.0
3,025,757	-	ygfQ	b4464	0.45	-0.6
3,025,863	-	ygfQ	b4464	0.53	-2.1
3,025,965	-	ygfQ	b4464	0.60	-1.5
3,025,965	-	ygfQ	b4464	0.60	-0.6
3,026,052	+	ygfQ	b4464	0.66	+1.4
3,026,088	+	ygfQ	b4464	0.69	+0.6
3,026,137	+	ygfQ	b4464	0.73	+0.6
3,026,137	+	ygfQ	b4464	0.73	+0.2
3,026,261	-				+0.4
3,026,261	-	ygfQ	b4464	0.82	-1.5
3,026,263	+	ygfQ	b4464	0.82	-0.1
3,026,321	+	ygfQ	b4464	0.86	+0.4
3,026,321	+	ygfQ	b4464	0.86	-1.4
3,026,331	+	ygfQ	b4464	0.87	+1.6
3,026,363	-	ygfQ	b4464	0.89	+0.1
3,026,414	-				-1.6
3,026,467	-				+2.2
3,026,623	+	ygfS	b2886	0.16	-2.4
3,026,641	+	ygfS	b2886	0.19	+0.8
3,026,662	+	ygfS	b2886	0.24	-0.9
3,026,705	+	ygfS	b2886	0.33	-1.4
3,026,723	-	ygfS	b2886	0.36	+0.2
3,026,800	-	ygfS	b2886	0.52	-1.6
3,026,800	-	ygfS	b2886	0.52	+3.4
3,026,812	-	ygfS	b2886	0.54	-2.1
3,026,853	+	ygfS	b2886	0.63	+0.9
3,026,947	+	ygfS	b2886	0.82	-1.1
3,026,947	+	ygfS	b2886	0.82	+3.3
3,027,136	-				+1.6
3,027,136	-				+0.5
3,027,184	-				+1.9
3,027,255	-	ygfT	b2887	0.12	+0.0
3,027,255	-	ygfT	b2887	0.12	+0.2
3,027,337	+	ygfT	b2887	0.16	-1.5
3,027,370	-	ygfT	b2887	0.17	+2.0
3,027,380	-	ygfT	b2887	0.18	+1.1
3,027,407	+	ygfT	b2887	0.19	+0.9
3,027,466	-	ygfT	b2887	0.23	-0.9