Experiment: LB Anaerobic with Nitrofurantoin 0.00003125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ttdA and ttdB overlap by 4 nucleotides ttdB and ygjE are separated by 47 nucleotides
b3061: ttdA - tartrate dehydratase (NCBI), at 3,204,485 to 3,205,396
ttdA
b3062: ttdB - L(+)-tartrate dehydratase (NCBI), at 3,205,393 to 3,205,998
ttdB
b3063: ygjE - predicted tartrate:succinate antiporter (NCBI), at 3,206,046 to 3,207,509
ygjE
Position (kb)
3205
3206 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3204.394 kb on - strand at 3204.394 kb on - strand at 3204.490 kb on - strand at 3204.490 kb on - strand at 3204.506 kb on - strand at 3204.506 kb on - strand at 3204.525 kb on - strand at 3204.525 kb on - strand at 3204.530 kb on + strand at 3204.572 kb on + strand at 3204.572 kb on + strand at 3204.572 kb on + strand at 3204.573 kb on + strand at 3204.573 kb on + strand at 3204.579 kb on - strand, within ttdA at 3204.579 kb on - strand, within ttdA at 3204.580 kb on - strand, within ttdA at 3204.580 kb on - strand, within ttdA at 3204.581 kb on - strand, within ttdA at 3204.581 kb on - strand, within ttdA at 3204.584 kb on + strand, within ttdA at 3204.586 kb on + strand, within ttdA at 3204.591 kb on + strand, within ttdA at 3204.615 kb on - strand, within ttdA at 3204.615 kb on - strand, within ttdA at 3204.619 kb on + strand, within ttdA at 3204.642 kb on + strand, within ttdA at 3204.650 kb on - strand, within ttdA at 3204.668 kb on - strand, within ttdA at 3204.668 kb on - strand, within ttdA at 3204.668 kb on - strand, within ttdA at 3204.685 kb on - strand, within ttdA at 3204.685 kb on - strand, within ttdA at 3204.685 kb on - strand, within ttdA at 3204.685 kb on - strand, within ttdA at 3204.710 kb on - strand, within ttdA at 3204.721 kb on - strand, within ttdA at 3204.852 kb on - strand, within ttdA at 3204.852 kb on - strand, within ttdA at 3204.852 kb on - strand, within ttdA at 3204.852 kb on - strand, within ttdA at 3204.888 kb on + strand, within ttdA at 3204.895 kb on - strand, within ttdA at 3204.895 kb on - strand, within ttdA at 3204.896 kb on - strand, within ttdA at 3204.953 kb on + strand, within ttdA at 3204.982 kb on - strand, within ttdA at 3205.061 kb on + strand, within ttdA at 3205.185 kb on + strand, within ttdA at 3205.185 kb on + strand, within ttdA at 3205.222 kb on - strand, within ttdA at 3205.252 kb on - strand, within ttdA at 3205.252 kb on - strand, within ttdA at 3205.293 kb on + strand, within ttdA at 3205.296 kb on + strand, within ttdA at 3205.296 kb on + strand, within ttdA at 3205.345 kb on - strand at 3205.345 kb on - strand at 3205.372 kb on + strand at 3205.380 kb on - strand at 3205.401 kb on - strand at 3205.495 kb on - strand, within ttdB at 3205.627 kb on - strand, within ttdB at 3205.783 kb on - strand, within ttdB at 3205.823 kb on - strand, within ttdB at 3205.823 kb on - strand, within ttdB at 3205.846 kb on + strand, within ttdB at 3205.846 kb on + strand, within ttdB at 3206.003 kb on + strand at 3206.011 kb on - strand at 3206.011 kb on - strand at 3206.046 kb on + strand at 3206.046 kb on + strand at 3206.068 kb on + strand at 3206.084 kb on + strand at 3206.155 kb on - strand at 3206.155 kb on - strand at 3206.233 kb on - strand, within ygjE at 3206.241 kb on + strand, within ygjE at 3206.251 kb on + strand, within ygjE at 3206.259 kb on - strand, within ygjE at 3206.264 kb on + strand, within ygjE at 3206.482 kb on + strand, within ygjE at 3206.482 kb on + strand, within ygjE at 3206.482 kb on + strand at 3206.503 kb on + strand, within ygjE at 3206.513 kb on + strand, within ygjE at 3206.578 kb on - strand, within ygjE at 3206.608 kb on + strand, within ygjE at 3206.608 kb on + strand, within ygjE at 3206.613 kb on + strand, within ygjE at 3206.625 kb on - strand, within ygjE at 3206.640 kb on + strand, within ygjE at 3206.648 kb on + strand, within ygjE at 3206.716 kb on - strand, within ygjE at 3206.735 kb on + strand, within ygjE at 3206.735 kb on + strand, within ygjE at 3206.738 kb on - strand, within ygjE at 3206.983 kb on + strand, within ygjE at 3206.983 kb on + strand, within ygjE
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Nitrofurantoin 0.00003125 mM remove 3,204,394 - +3.7 3,204,394 - -1.6 3,204,490 - +1.0 3,204,490 - +2.3 3,204,506 - -2.2 3,204,506 - +1.4 3,204,525 - -0.4 3,204,525 - -0.0 3,204,530 + -0.2 3,204,572 + -1.0 3,204,572 + +0.4 3,204,572 + -1.2 3,204,573 + +1.4 3,204,573 + -0.6 3,204,579 - ttdA b3061 0.10 -0.6 3,204,579 - ttdA b3061 0.10 -1.3 3,204,580 - ttdA b3061 0.10 -0.8 3,204,580 - ttdA b3061 0.10 -0.9 3,204,581 - ttdA b3061 0.11 +3.5 3,204,581 - ttdA b3061 0.11 -0.8 3,204,584 + ttdA b3061 0.11 +0.0 3,204,586 + ttdA b3061 0.11 -1.3 3,204,591 + ttdA b3061 0.12 +1.4 3,204,615 - ttdA b3061 0.14 +1.1 3,204,615 - ttdA b3061 0.14 -0.6 3,204,619 + ttdA b3061 0.15 -0.2 3,204,642 + ttdA b3061 0.17 +2.3 3,204,650 - ttdA b3061 0.18 -0.8 3,204,668 - ttdA b3061 0.20 -1.6 3,204,668 - ttdA b3061 0.20 -3.0 3,204,668 - ttdA b3061 0.20 +3.0 3,204,685 - ttdA b3061 0.22 +1.0 3,204,685 - ttdA b3061 0.22 +0.4 3,204,685 - ttdA b3061 0.22 +0.8 3,204,685 - ttdA b3061 0.22 -3.0 3,204,710 - ttdA b3061 0.25 -3.2 3,204,721 - ttdA b3061 0.26 +0.0 3,204,852 - ttdA b3061 0.40 -1.0 3,204,852 - ttdA b3061 0.40 -2.2 3,204,852 - ttdA b3061 0.40 -0.3 3,204,852 - ttdA b3061 0.40 +1.9 3,204,888 + ttdA b3061 0.44 -0.1 3,204,895 - ttdA b3061 0.45 +0.1 3,204,895 - ttdA b3061 0.45 +1.4 3,204,896 - ttdA b3061 0.45 -1.8 3,204,953 + ttdA b3061 0.51 -1.5 3,204,982 - ttdA b3061 0.54 +2.0 3,205,061 + ttdA b3061 0.63 -0.5 3,205,185 + ttdA b3061 0.77 +0.8 3,205,185 + ttdA b3061 0.77 +2.6 3,205,222 - ttdA b3061 0.81 -0.8 3,205,252 - ttdA b3061 0.84 -0.1 3,205,252 - ttdA b3061 0.84 +0.0 3,205,293 + ttdA b3061 0.89 -1.0 3,205,296 + ttdA b3061 0.89 +0.7 3,205,296 + ttdA b3061 0.89 +1.0 3,205,345 - -1.6 3,205,345 - +0.5 3,205,372 + +1.4 3,205,380 - -2.2 3,205,401 - -0.7 3,205,495 - ttdB b3062 0.17 -0.2 3,205,627 - ttdB b3062 0.39 +3.7 3,205,783 - ttdB b3062 0.64 +0.5 3,205,823 - ttdB b3062 0.71 +0.0 3,205,823 - ttdB b3062 0.71 +1.3 3,205,846 + ttdB b3062 0.75 +1.2 3,205,846 + ttdB b3062 0.75 +1.4 3,206,003 + +1.2 3,206,011 - +1.4 3,206,011 - +0.2 3,206,046 + -0.1 3,206,046 + +0.1 3,206,068 + +1.0 3,206,084 + -0.6 3,206,155 - -1.4 3,206,155 - +1.0 3,206,233 - ygjE b3063 0.13 +0.5 3,206,241 + ygjE b3063 0.13 -0.9 3,206,251 + ygjE b3063 0.14 +0.1 3,206,259 - ygjE b3063 0.15 -0.6 3,206,264 + ygjE b3063 0.15 +0.5 3,206,482 + ygjE b3063 0.30 +2.0 3,206,482 + ygjE b3063 0.30 -0.4 3,206,482 + -0.2 3,206,503 + ygjE b3063 0.31 -0.3 3,206,513 + ygjE b3063 0.32 +0.1 3,206,578 - ygjE b3063 0.36 -0.2 3,206,608 + ygjE b3063 0.38 +0.3 3,206,608 + ygjE b3063 0.38 +0.6 3,206,613 + ygjE b3063 0.39 +0.4 3,206,625 - ygjE b3063 0.40 +1.0 3,206,640 + ygjE b3063 0.41 -0.5 3,206,648 + ygjE b3063 0.41 +2.1 3,206,716 - ygjE b3063 0.46 -1.6 3,206,735 + ygjE b3063 0.47 +1.2 3,206,735 + ygjE b3063 0.47 -1.3 3,206,738 - ygjE b3063 0.47 +0.1 3,206,983 + ygjE b3063 0.64 +0.4 3,206,983 + ygjE b3063 0.64 +0.5
Or see this region's nucleotide sequence