Strain Fitness in Escherichia coli BW25113 around b2540

Experiment: LB Anaerobic with Nitrofurantoin 0.00003125 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nthcaE and hcaF overlap by 4 nucleotideshcaF and hcaC overlap by 1 nucleotideshcaC and hcaB overlap by 4 nucleotideshcaB and hcaD are separated by 9 nucleotides b2538: hcaE - 3-phenylpropionate dioxygenase, large (alpha) subunit (NCBI), at 2,667,054 to 2,668,415 hcaE b2539: hcaF - 3-phenylpropionate dioxygenase, small (beta) subunit (NCBI), at 2,668,412 to 2,668,930 hcaF b2540: hcaC - 3-phenylpropionate dioxygenase, predicted ferredoxin subunit (NCBI), at 2,668,930 to 2,669,250 hcaC b2541: hcaB - 2,3-dihydroxy-2,3-dihydrophenylpropionate dehydrogenase (NCBI), at 2,669,247 to 2,670,059 hcaB b2542: hcaD - phenylpropionate dioxygenase, ferredoxin reductase subunit (NCBI), at 2,670,069 to 2,671,271 hcaD Position (kb) 2668 2669 2670Strain fitness (log2 ratio) -2 -1 0 1 2 3at 2667.930 kb on - strand, within hcaEat 2667.956 kb on + strand, within hcaEat 2668.118 kb on - strand, within hcaEat 2668.174 kb on - strand, within hcaEat 2668.253 kb on + strand, within hcaEat 2668.253 kb on + strand, within hcaEat 2668.363 kb on + strandat 2668.539 kb on - strand, within hcaFat 2668.551 kb on - strand, within hcaFat 2668.551 kb on - strand, within hcaFat 2668.676 kb on + strand, within hcaFat 2668.676 kb on + strand, within hcaFat 2668.709 kb on + strand, within hcaFat 2668.715 kb on + strand, within hcaFat 2668.736 kb on - strand, within hcaFat 2668.759 kb on + strand, within hcaFat 2668.888 kb on - strandat 2668.888 kb on - strandat 2668.925 kb on - strandat 2668.932 kb on - strandat 2668.979 kb on - strand, within hcaCat 2669.083 kb on + strand, within hcaCat 2669.112 kb on - strand, within hcaCat 2669.166 kb on + strand, within hcaCat 2669.295 kb on + strandat 2669.442 kb on - strand, within hcaBat 2669.469 kb on - strandat 2669.662 kb on + strand, within hcaBat 2669.678 kb on - strand, within hcaBat 2669.678 kb on - strand, within hcaBat 2669.716 kb on - strand, within hcaBat 2669.716 kb on - strand, within hcaBat 2669.827 kb on + strand, within hcaBat 2669.827 kb on + strand, within hcaBat 2669.835 kb on - strand, within hcaBat 2669.835 kb on - strand, within hcaBat 2669.946 kb on + strand, within hcaBat 2669.947 kb on - strand, within hcaBat 2669.951 kb on + strand, within hcaBat 2669.951 kb on + strand, within hcaBat 2670.153 kb on + strandat 2670.199 kb on + strand, within hcaD

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Anaerobic with Nitrofurantoin 0.00003125 mM
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2,667,930 - hcaE b2538 0.64 +0.9
2,667,956 + hcaE b2538 0.66 -0.1
2,668,118 - hcaE b2538 0.78 -0.4
2,668,174 - hcaE b2538 0.82 +0.4
2,668,253 + hcaE b2538 0.88 -0.0
2,668,253 + hcaE b2538 0.88 +0.2
2,668,363 + -0.9
2,668,539 - hcaF b2539 0.24 +0.4
2,668,551 - hcaF b2539 0.27 -2.7
2,668,551 - hcaF b2539 0.27 -0.6
2,668,676 + hcaF b2539 0.51 -0.0
2,668,676 + hcaF b2539 0.51 -1.4
2,668,709 + hcaF b2539 0.57 -0.8
2,668,715 + hcaF b2539 0.58 -1.1
2,668,736 - hcaF b2539 0.62 -0.3
2,668,759 + hcaF b2539 0.67 -0.2
2,668,888 - -1.4
2,668,888 - -0.4
2,668,925 - -2.4
2,668,932 - -0.1
2,668,979 - hcaC b2540 0.15 -2.3
2,669,083 + hcaC b2540 0.48 +1.2
2,669,112 - hcaC b2540 0.57 +0.6
2,669,166 + hcaC b2540 0.74 -0.8
2,669,295 + -0.2
2,669,442 - hcaB b2541 0.24 -1.4
2,669,469 - -0.4
2,669,662 + hcaB b2541 0.51 +1.0
2,669,678 - hcaB b2541 0.53 +0.7
2,669,678 - hcaB b2541 0.53 +3.4
2,669,716 - hcaB b2541 0.58 -1.4
2,669,716 - hcaB b2541 0.58 +1.9
2,669,827 + hcaB b2541 0.71 +2.1
2,669,827 + hcaB b2541 0.71 +0.7
2,669,835 - hcaB b2541 0.72 -0.4
2,669,835 - hcaB b2541 0.72 -0.6
2,669,946 + hcaB b2541 0.86 -2.2
2,669,947 - hcaB b2541 0.86 +0.3
2,669,951 + hcaB b2541 0.87 -0.5
2,669,951 + hcaB b2541 0.87 -1.8
2,670,153 + -0.3
2,670,199 + hcaD b2542 0.11 -0.4

Or see this region's nucleotide sequence