Experiment: LB Anaerobic with Nitrofurantoin 0.00003125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yfaS and b2229 overlap by 67 nucleotides b2229 and yfaA overlap by 4 nucleotides yfaA and gyrA are separated by 148 nucleotides
b4500: yfaS - no description, at 2,327,820 to 2,332,424
yfaS
b2229: b2229 - orf, hypothetical protein (VIMSS), at 2,332,358 to 2,332,981
b2229
b2230: yfaA - orf, hypothetical protein (VIMSS), at 2,332,978 to 2,334,666
yfaA
b2231: gyrA - DNA gyrase subunit A (NCBI), at 2,334,815 to 2,337,442
gyrA
Position (kb)
2332
2333
2334
2335 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2332.014 kb on + strand at 2332.020 kb on + strand at 2332.020 kb on + strand at 2332.048 kb on - strand at 2332.048 kb on - strand at 2332.166 kb on + strand at 2332.169 kb on - strand at 2332.169 kb on - strand at 2332.223 kb on + strand at 2332.230 kb on - strand at 2332.231 kb on - strand at 2332.448 kb on - strand, within b2229 at 2332.531 kb on + strand, within b2229 at 2332.531 kb on + strand, within b2229 at 2332.697 kb on - strand, within b2229 at 2332.834 kb on + strand, within b2229 at 2332.876 kb on + strand, within b2229 at 2332.892 kb on + strand, within b2229 at 2332.944 kb on - strand at 2333.058 kb on - strand at 2333.072 kb on - strand at 2333.072 kb on - strand at 2333.184 kb on + strand, within yfaA at 2333.184 kb on + strand, within yfaA at 2333.184 kb on + strand, within yfaA at 2333.215 kb on + strand, within yfaA at 2333.326 kb on - strand, within yfaA at 2333.527 kb on + strand, within yfaA at 2333.588 kb on + strand, within yfaA at 2333.588 kb on + strand, within yfaA at 2333.608 kb on + strand, within yfaA at 2333.609 kb on - strand, within yfaA at 2333.619 kb on - strand, within yfaA at 2333.619 kb on - strand, within yfaA at 2333.619 kb on - strand, within yfaA at 2333.621 kb on + strand, within yfaA at 2333.621 kb on + strand, within yfaA at 2333.658 kb on - strand, within yfaA at 2333.697 kb on + strand, within yfaA at 2333.697 kb on + strand, within yfaA at 2333.725 kb on + strand, within yfaA at 2334.078 kb on - strand, within yfaA at 2334.213 kb on - strand, within yfaA at 2334.241 kb on - strand, within yfaA at 2334.270 kb on + strand, within yfaA at 2334.289 kb on + strand, within yfaA at 2334.334 kb on + strand, within yfaA at 2334.462 kb on + strand, within yfaA at 2334.632 kb on - strand at 2334.632 kb on - strand at 2334.688 kb on - strand at 2334.842 kb on - strand at 2334.902 kb on - strand at 2334.902 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Nitrofurantoin 0.00003125 mM remove 2,332,014 + -1.2 2,332,020 + +0.1 2,332,020 + -0.9 2,332,048 - -1.4 2,332,048 - +0.0 2,332,166 + +0.2 2,332,169 - +0.4 2,332,169 - +0.2 2,332,223 + -0.9 2,332,230 - +1.5 2,332,231 - +0.8 2,332,448 - b2229 b2229 0.14 +2.9 2,332,531 + b2229 b2229 0.28 +0.3 2,332,531 + b2229 b2229 0.28 +0.8 2,332,697 - b2229 b2229 0.54 +0.1 2,332,834 + b2229 b2229 0.76 -1.9 2,332,876 + b2229 b2229 0.83 +0.5 2,332,892 + b2229 b2229 0.86 -0.2 2,332,944 - +0.9 2,333,058 - -1.6 2,333,072 - +2.8 2,333,072 - +0.6 2,333,184 + yfaA b2230 0.12 +0.1 2,333,184 + yfaA b2230 0.12 -2.7 2,333,184 + yfaA b2230 0.12 -0.9 2,333,215 + yfaA b2230 0.14 +1.6 2,333,326 - yfaA b2230 0.21 +2.7 2,333,527 + yfaA b2230 0.33 -0.3 2,333,588 + yfaA b2230 0.36 +2.5 2,333,588 + yfaA b2230 0.36 +1.6 2,333,608 + yfaA b2230 0.37 -1.4 2,333,609 - yfaA b2230 0.37 +0.5 2,333,619 - yfaA b2230 0.38 +0.5 2,333,619 - yfaA b2230 0.38 +1.1 2,333,619 - yfaA b2230 0.38 -1.2 2,333,621 + yfaA b2230 0.38 +0.3 2,333,621 + yfaA b2230 0.38 +1.1 2,333,658 - yfaA b2230 0.40 +2.1 2,333,697 + yfaA b2230 0.43 +0.6 2,333,697 + yfaA b2230 0.43 +1.3 2,333,725 + yfaA b2230 0.44 +0.8 2,334,078 - yfaA b2230 0.65 +2.5 2,334,213 - yfaA b2230 0.73 -1.7 2,334,241 - yfaA b2230 0.75 -2.4 2,334,270 + yfaA b2230 0.76 +1.0 2,334,289 + yfaA b2230 0.78 +1.1 2,334,334 + yfaA b2230 0.80 -1.6 2,334,462 + yfaA b2230 0.88 -1.4 2,334,632 - +0.1 2,334,632 - +0.1 2,334,688 - -0.7 2,334,842 - -1.2 2,334,902 - -0.4 2,334,902 - +1.7
Or see this region's nucleotide sequence