Experiment: LB Anaerobic with Nitrofurantoin 0.00003125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt fruB and setB are separated by 367 nucleotides setB and yeiW overlap by 4 nucleotides yeiW and yeiP are separated by 154 nucleotides
b2169: fruB - fused fructose-specific PTS enzymes: IIA component/HPr component (NCBI), at 2,260,387 to 2,261,517
fruB
b2170: setB - lactose/glucose efflux system (NCBI), at 2,261,885 to 2,263,066
setB
b4502: yeiW - hypothetical protein (NCBI), at 2,263,063 to 2,263,317
yeiW
b2171: yeiP - putative elongation factor (VIMSS), at 2,263,472 to 2,264,044
yeiP
Position (kb)
2261
2262
2263
2264 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2260.911 kb on + strand, within fruB at 2260.911 kb on + strand, within fruB at 2260.938 kb on + strand, within fruB at 2261.006 kb on - strand, within fruB at 2261.202 kb on + strand, within fruB at 2261.241 kb on + strand, within fruB at 2261.241 kb on + strand, within fruB at 2261.255 kb on + strand, within fruB at 2261.330 kb on - strand, within fruB at 2261.476 kb on - strand at 2261.937 kb on - strand at 2262.160 kb on - strand, within setB at 2262.373 kb on - strand, within setB at 2262.590 kb on - strand, within setB at 2262.590 kb on - strand, within setB at 2262.752 kb on - strand, within setB at 2262.916 kb on + strand, within setB at 2262.925 kb on - strand, within setB at 2263.022 kb on + strand at 2263.022 kb on + strand at 2263.060 kb on + strand at 2263.088 kb on - strand at 2263.102 kb on - strand, within yeiW at 2263.102 kb on - strand, within yeiW at 2263.129 kb on + strand, within yeiW at 2263.136 kb on + strand, within yeiW at 2263.213 kb on - strand, within yeiW at 2263.213 kb on - strand, within yeiW at 2263.217 kb on + strand, within yeiW at 2263.286 kb on - strand, within yeiW at 2263.321 kb on - strand at 2263.563 kb on + strand, within yeiP at 2263.563 kb on + strand, within yeiP at 2263.575 kb on + strand, within yeiP at 2263.743 kb on - strand, within yeiP at 2263.813 kb on + strand, within yeiP at 2263.826 kb on + strand, within yeiP at 2263.826 kb on + strand, within yeiP at 2263.908 kb on - strand, within yeiP at 2263.929 kb on - strand, within yeiP at 2263.960 kb on - strand, within yeiP at 2264.034 kb on - strand at 2264.039 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Nitrofurantoin 0.00003125 mM remove 2,260,911 + fruB b2169 0.46 -1.0 2,260,911 + fruB b2169 0.46 -2.1 2,260,938 + fruB b2169 0.49 +0.2 2,261,006 - fruB b2169 0.55 +0.5 2,261,202 + fruB b2169 0.72 +0.3 2,261,241 + fruB b2169 0.76 +2.5 2,261,241 + fruB b2169 0.76 +0.0 2,261,255 + fruB b2169 0.77 -1.1 2,261,330 - fruB b2169 0.83 +1.2 2,261,476 - -0.1 2,261,937 - +2.0 2,262,160 - setB b2170 0.23 -0.3 2,262,373 - setB b2170 0.41 -0.1 2,262,590 - setB b2170 0.60 -1.0 2,262,590 - setB b2170 0.60 -0.1 2,262,752 - setB b2170 0.73 -1.3 2,262,916 + setB b2170 0.87 -0.9 2,262,925 - setB b2170 0.88 -2.1 2,263,022 + -0.4 2,263,022 + -0.0 2,263,060 + +0.0 2,263,088 - -3.1 2,263,102 - yeiW b4502 0.15 +0.6 2,263,102 - yeiW b4502 0.15 +0.3 2,263,129 + yeiW b4502 0.26 -1.9 2,263,136 + yeiW b4502 0.29 +1.9 2,263,213 - yeiW b4502 0.59 +1.5 2,263,213 - yeiW b4502 0.59 -0.5 2,263,217 + yeiW b4502 0.60 +1.0 2,263,286 - yeiW b4502 0.87 +2.3 2,263,321 - -0.5 2,263,563 + yeiP b2171 0.16 +1.8 2,263,563 + yeiP b2171 0.16 +1.0 2,263,575 + yeiP b2171 0.18 +0.4 2,263,743 - yeiP b2171 0.47 -0.3 2,263,813 + yeiP b2171 0.60 +1.4 2,263,826 + yeiP b2171 0.62 +0.8 2,263,826 + yeiP b2171 0.62 -1.8 2,263,908 - yeiP b2171 0.76 -0.3 2,263,929 - yeiP b2171 0.80 +0.8 2,263,960 - yeiP b2171 0.85 +0.2 2,264,034 - -1.0 2,264,039 + -0.5
Or see this region's nucleotide sequence