Experiment: LB Anaerobic with Nitrofurantoin 0.00003125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt galS and yeiB are separated by 141 nucleotides yeiB and folE are separated by 16 nucleotides folE and yeiG are separated by 257 nucleotides
b2151: galS - DNA-binding transcriptional repressor (NCBI), at 2,238,650 to 2,239,690
galS
b2152: yeiB - conserved inner membrane protein (NCBI), at 2,239,832 to 2,240,989
yeiB
b2153: folE - GTP cyclohydrolase I (NCBI), at 2,241,006 to 2,241,674
folE
b2154: yeiG - predicted esterase (NCBI), at 2,241,932 to 2,242,768
yeiG
Position (kb)
2239
2240
2241 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2238.839 kb on - strand, within galS at 2238.857 kb on - strand, within galS at 2239.012 kb on + strand, within galS at 2239.042 kb on - strand, within galS at 2239.066 kb on + strand, within galS at 2239.098 kb on - strand, within galS at 2239.098 kb on - strand, within galS at 2239.112 kb on - strand, within galS at 2239.267 kb on - strand, within galS at 2239.394 kb on + strand, within galS at 2239.429 kb on - strand, within galS at 2239.429 kb on - strand, within galS at 2239.452 kb on + strand, within galS at 2239.532 kb on + strand, within galS at 2239.590 kb on - strand at 2239.590 kb on - strand at 2239.605 kb on - strand at 2239.605 kb on - strand at 2239.699 kb on - strand at 2239.752 kb on - strand at 2239.937 kb on + strand at 2239.953 kb on + strand, within yeiB at 2240.119 kb on - strand, within yeiB at 2240.217 kb on + strand, within yeiB at 2240.262 kb on - strand, within yeiB at 2240.313 kb on - strand, within yeiB at 2240.566 kb on + strand, within yeiB at 2240.566 kb on + strand, within yeiB at 2240.778 kb on + strand, within yeiB at 2240.781 kb on + strand, within yeiB at 2240.781 kb on + strand, within yeiB at 2240.825 kb on - strand, within yeiB at 2240.882 kb on - strand at 2240.889 kb on + strand at 2240.889 kb on + strand at 2240.891 kb on - strand at 2241.735 kb on + strand at 2241.778 kb on - strand at 2241.778 kb on - strand at 2241.884 kb on - strand at 2241.884 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Nitrofurantoin 0.00003125 mM remove 2,238,839 - galS b2151 0.18 -0.0 2,238,857 - galS b2151 0.20 +0.7 2,239,012 + galS b2151 0.35 -2.0 2,239,042 - galS b2151 0.38 -0.5 2,239,066 + galS b2151 0.40 -0.1 2,239,098 - galS b2151 0.43 +0.5 2,239,098 - galS b2151 0.43 -0.0 2,239,112 - galS b2151 0.44 -1.3 2,239,267 - galS b2151 0.59 -2.1 2,239,394 + galS b2151 0.71 +1.1 2,239,429 - galS b2151 0.75 -2.4 2,239,429 - galS b2151 0.75 -1.2 2,239,452 + galS b2151 0.77 +1.1 2,239,532 + galS b2151 0.85 -0.6 2,239,590 - -0.9 2,239,590 - +0.6 2,239,605 - +1.4 2,239,605 - +0.1 2,239,699 - +1.3 2,239,752 - -0.8 2,239,937 + -2.6 2,239,953 + yeiB b2152 0.10 +1.4 2,240,119 - yeiB b2152 0.25 +0.8 2,240,217 + yeiB b2152 0.33 +0.1 2,240,262 - yeiB b2152 0.37 +1.6 2,240,313 - yeiB b2152 0.42 +1.0 2,240,566 + yeiB b2152 0.63 -2.7 2,240,566 + yeiB b2152 0.63 -2.7 2,240,778 + yeiB b2152 0.82 -0.4 2,240,781 + yeiB b2152 0.82 -0.3 2,240,781 + yeiB b2152 0.82 +2.1 2,240,825 - yeiB b2152 0.86 -0.8 2,240,882 - +0.3 2,240,889 + +1.0 2,240,889 + +1.3 2,240,891 - -0.7 2,241,735 + +0.1 2,241,778 - -0.5 2,241,778 - -0.7 2,241,884 - +2.0 2,241,884 - +0.1
Or see this region's nucleotide sequence