Strain Fitness in Escherichia coli BW25113 around b1582

Experiment: LB Anaerobic with Nitrofurantoin 0.00003125 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntrspA and ynfA are separated by 205 nucleotidesynfA and ynfB are separated by 134 nucleotidesynfB and speG are separated by 34 nucleotides b1581: rspA - predicted dehydratase (NCBI), at 1,651,951 to 1,653,165 rspA b1582: ynfA - hypothetical protein (NCBI), at 1,653,371 to 1,653,697 ynfA b1583: ynfB - hypothetical protein (NCBI), at 1,653,832 to 1,654,173 ynfB b1584: speG - spermidine N1-acetyltransferase (NCBI), at 1,654,208 to 1,654,768 speG Position (kb) 1653 1654Strain fitness (log2 ratio) -2 -1 0 1 2 3 4at 1652.628 kb on - strand, within rspAat 1652.940 kb on + strand, within rspAat 1653.008 kb on + strand, within rspAat 1653.070 kb on + strandat 1653.070 kb on + strandat 1653.166 kb on - strandat 1653.461 kb on + strand, within ynfAat 1653.557 kb on - strand, within ynfAat 1653.632 kb on + strand, within ynfAat 1653.757 kb on + strandat 1653.792 kb on + strandat 1653.800 kb on - strandat 1653.869 kb on + strand, within ynfBat 1653.869 kb on + strandat 1653.881 kb on + strand, within ynfBat 1654.127 kb on - strand, within ynfBat 1654.136 kb on + strand, within ynfBat 1654.144 kb on - strandat 1654.144 kb on - strandat 1654.204 kb on + strandat 1654.204 kb on + strandat 1654.205 kb on - strandat 1654.224 kb on + strandat 1654.263 kb on + strandat 1654.299 kb on - strand, within speGat 1654.339 kb on + strand, within speGat 1654.362 kb on + strand, within speGat 1654.391 kb on - strand, within speGat 1654.394 kb on + strand, within speGat 1654.394 kb on + strand, within speGat 1654.420 kb on + strand, within speGat 1654.486 kb on + strand, within speGat 1654.486 kb on + strand, within speGat 1654.499 kb on + strand, within speGat 1654.532 kb on + strand, within speGat 1654.532 kb on + strand, within speGat 1654.534 kb on - strand, within speGat 1654.534 kb on - strand, within speGat 1654.537 kb on + strand, within speGat 1654.537 kb on + strand, within speGat 1654.539 kb on + strand, within speGat 1654.539 kb on + strand, within speGat 1654.563 kb on + strand, within speGat 1654.563 kb on + strand, within speGat 1654.564 kb on + strand, within speGat 1654.578 kb on + strand, within speGat 1654.690 kb on + strand, within speGat 1654.690 kb on + strand, within speG

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Anaerobic with Nitrofurantoin 0.00003125 mM
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1,652,628 - rspA b1581 0.56 +0.4
1,652,940 + rspA b1581 0.81 -0.5
1,653,008 + rspA b1581 0.87 +2.7
1,653,070 + +1.1
1,653,070 + -0.5
1,653,166 - +4.6
1,653,461 + ynfA b1582 0.28 -2.1
1,653,557 - ynfA b1582 0.57 +0.6
1,653,632 + ynfA b1582 0.80 -1.7
1,653,757 + +0.2
1,653,792 + -0.9
1,653,800 - +0.4
1,653,869 + ynfB b1583 0.11 +1.7
1,653,869 + +1.1
1,653,881 + ynfB b1583 0.14 +1.6
1,654,127 - ynfB b1583 0.86 -0.5
1,654,136 + ynfB b1583 0.89 +2.3
1,654,144 - +1.1
1,654,144 - +1.1
1,654,204 + -0.9
1,654,204 + +1.7
1,654,205 - +1.1
1,654,224 + +0.5
1,654,263 + +0.5
1,654,299 - speG b1584 0.16 -1.5
1,654,339 + speG b1584 0.23 -0.6
1,654,362 + speG b1584 0.27 +1.4
1,654,391 - speG b1584 0.33 +1.1
1,654,394 + speG b1584 0.33 -1.7
1,654,394 + speG b1584 0.33 +1.1
1,654,420 + speG b1584 0.38 +0.4
1,654,486 + speG b1584 0.50 -1.7
1,654,486 + speG b1584 0.50 +0.5
1,654,499 + speG b1584 0.52 -0.6
1,654,532 + speG b1584 0.58 -1.7
1,654,532 + speG b1584 0.58 -1.1
1,654,534 - speG b1584 0.58 +0.1
1,654,534 - speG b1584 0.58 -1.7
1,654,537 + speG b1584 0.59 -0.9
1,654,537 + speG b1584 0.59 -0.1
1,654,539 + speG b1584 0.59 -0.4
1,654,539 + speG b1584 0.59 -1.1
1,654,563 + speG b1584 0.63 -0.3
1,654,563 + speG b1584 0.63 -0.2
1,654,564 + speG b1584 0.63 -1.7
1,654,578 + speG b1584 0.66 -2.1
1,654,690 + speG b1584 0.86 -1.5
1,654,690 + speG b1584 0.86 -0.5

Or see this region's nucleotide sequence